Çiftliklerden Alınan İnek Sütlerinde Genişlemiş Spektrumlu Beta-Laktamaz Üreten Escherichia Coli'nin Araştırılması

Abstract

Bu çalışma, sığır çiftliği süt karışımlarında genişlemiş spektrumlu beta-laktamaz (ESBL) üreten Escherichia coli'nin varlığını araştırmak amacıyla yapıldı. Burdur ili Gölhisar ilçesindeki 32 çiftlikten alınan 32 çiftlik sütü, 2 μg/ml sefotaksim veya 2 μg/ml seftazidim ilave edilmiş MacConkey agar besi yerlerine eş zamanlı olarak ekildi. Aerobik şartlarda 37°C'de 24 saat inkübasyonun ardından, kültürlerde E. coli'nin tanımlanması amacıyla laktoz pozitif kolonilere biyokimyasal/fizyolojik testler uygulandı. Daha sonra E. coli izolatında ESBL üretiminin tespiti kombine disk yöntemiyle yapıldı. ESBL sınıfını belirlemek amacıyla, izolatlarda CTX-M, SHV ve TEM genleri polimeraz zincir reaksiyonu (PZR) yöntemiyle tarandı. Süt örneklerinden selektif kültür yapıldıktan sonra 1 adet (1/32, %3,12) E. coli izole edildi. Kombine disk yöntemiyle ESBL ürettiği doğrulanan izolatın CTX-M grup 1 sınıf ESBL genini taşıdığı, TEM ve SHV sınıflarına sahip olmadığı belirlendi. Bu çalışma, bölgedeki sığır işletmelerinin çiftlik sütlerinde ESBL üreten E. coli'nin düşük düzeyde bulunabileceğini göstermiştir. Bu çalışmada bir çiftlikten elde edilen çiftlik sütü karışımında tespit edilen ESBL üreten E. coli izolatının kaynağı araştırılmamış olsa da, tüketilecek sütte bu bakterinin tespit edilmiş olması önemlidir.

Keywords

• beta-laktamaz
• sığır sütü
• CTX-M geni
• Genişletilmiş spektrumlu beta-laktamaz
• Escherichia coli.

Investigation of Extended-Spectrum Beta-Lactamase Producing Escherichia Coli in Milk on Dairy Cattle Farm

Abstract

The present study was conducted to investigate the presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) in cattle farm milk mixtures. Thirty two farm milks from 32 farms in Gölhisar district of Burdur city (Türkiye) were concurrently cultured on MacConkey agar plates supplemented with either 2 μg/mL cefotaxime or 2 μg/mL ceftazidime. After incubation at 37°C for 24 h under aerobic conditions, biochemical/physiological tests were applied to the lactose positive colonies for identification of E. coli in the cultures. Subsequently, determination of ESBL production in E. coli isolate was done by the combined disk method. In order to determine the ESBL class, the isolates were screened for CTX-M, SHV and TEM genes by polymerase chain reaction (PCR). After the selective culture of the milk samples, one (1/32, 3.12%) E. coli was isolated. The isolate confirmed as an ESBL procedure by the combined disc method harbored the CTX-M group 1 ESBL gene, but tested negative for TEM and SHV genes. This study showed that ESBL-producing E. coli could be found at low level in farm milks of the cattle farms in the region. Although the source of the ESBL-producing E. coli isolated from the farm milk mixture was not identified in this study, it is important that this bacterium was detected in the milk to be consumed.

Keywords

• beta-lactamase
• cattle milk
• CTX-M gene
• Extended-spectrum beta-lactamase
• Escherichia coli.

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