Clinical Research
BibTex RIS Cite

The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats

Year 2022, Volume: 17 Issue: 2, 41 - 44, 01.10.2022
https://doi.org/10.5152/VetSciPract.2022.221118

Abstract

This study aimed to investigate the effect of vancomycin on sperm motility and testicular inflammation in male rats. In the study, 12 adult Sprague Dawley rats were used. They were divided into 2 equal groups. First group (n = 6) was called as control group. Control group received physiological saline via intraperitoneal injection. Second group was called as vancomycin group and vancomycin group received vancomycin at the dose of 200 mg/kg via intraperitoneal injection for consecutive 7 days. At the end of study, the rats were sacrificed under sevoflurane anesthesia. Testes and cauda epididymis were collected. Mean testes weight, mean cauda epididymis weight, and sperm motility were evaluated. Also, tumor necrosis factor-alpha and nuclear factor kappa B levels were evaluated in testis tissues. According to our results, vancomycin treatment significantly decreased sperm motility while did not change mean testis weight and mean cauda epididymis weight when compared to control group. Besides, tumor necrosis factor-alpha and nuclear factor kappa B levels were significantly higher in vancomycin group than in the control group. In conclusion, vancomycin treatment (200 mg/kg BW, consecutive 7 days) decreases sperm motility significantly and increases the levels of testicular inflammation biomarkers (tumor necrosis factor-alpha and nuclear factor kappa B). Further studies are needed for protection from the side effects of vancomycin in male reproductive system.

References

  • 1. Álvarez R, López Cortés LEL, Molina J, Cisneros JM, Pachón J. Optimizing the clinical use of vancomycin. Antimicrob Agents Chemother. 2016;60(5):2601-2609.
  • 2. Kucukler S, Darendelioğlu E, Caglayan C, Ayna A, Yıldırım S, Kandemir FM. Zingerone attenuates vancomycin-induced hepatotoxicity in rats through regulation of oxidative stress, inflammation and apoptosis. Life Sci. 2020;259:118382.
  • 3. Kandemir FM, Yildirim S, Kucukler S, Caglayan C, Mahamadu A, Dortbudak MB. Therapeutic efficacy of zingerone against vancomycin-induced oxidative stress, inflammation, apoptosis and aquaporin 1 permeability in rat kidney. Biomed Pharmacother. 2018;105:981-991.
  • 4. Naidu ECS, Olojede SO, Lawal SK, Peter AI, Akang EA, Azu OO. Effects of vancomycin linoleic acid nanoparticles on male reproductive indices of Sprague–Dawley rats. Artif Cells Nanomed Biotechnol. 2021;49(1):587-595.
  • 5. Butterfield TA, Best TM, Merrick MA. The dual roles of neutrophils and macrophages in inflammation: a critical balance between tissue damage and repair. J Athl Train. 2006;41(4):457-465.
  • 6. Zhang P, Zheng Y, Lv Y, et al. Melatonin protects the mouse testis against heat-induced damage. Mol Hum Reprod. 2020;26(2):65-79.
  • 7. Aksu EH, Akman O, Ömür AD, et al. 3, 3 diindolylmethane leads to apoptosis, decreases sperm quality, affects blood estradiol 17 β and testosterone, oestrogen (α and β) and androgen receptor levels in the reproductive system in male rats. Andrologia. 2016;48(10): 1155-1165.
  • 8. Dutta S, Sengupta P, Slama P, Roychoudhury S. Oxidative stress, testicular inflammatory pathways, and male reproduction. Int J Mol Sci. 2021;22(18):10043.
  • 9. Yang D, Elner SG, Bian ZM, Till GO, Petty HR, Elner VM. Pro-inflammatory cytokines increase reactive oxygen species through mitochondria and NADPH oxidase in cultured RPE cells. Exp Eye Res. 2007;85(4):462-472.
  • 10. Anderson MT, Staal FJ, Gitler C, Herzenberg LA, Herzenberg LA. Separation of oxidant-initiated and redox-regulated steps in the NFkappa B signal transduction pathway. Proc Natl Acad Sci U S A. 1994;91(24):11527-11531.
  • 11. Hales DB, Diemer T, Hales KH. Role of cytokines in testicular function. Endocrine. 1999;10(3):201-217.
  • 12. Feuerstein GZ, Liu T, Barone FC. Cytokines, inflammation, and brain injury: role of tumor necrosis factor-alpha. Cerebrovasc Brain Metab Rev. 1994;6(4):341-360.
  • 13. Cudicini C, Lejeune H, Gomez E, et al. Human Leydig cells and Sertoli cells are producers of interleukins-1 and -6. J Clin Endocrinol Metab. 1997;82(5):1426-1433.
  • 14. Zhang T, Ma C, Zhang Z, Zhang H, Hu H. NF-κB signalling in inflammation and cancer. MedComm. 2021;2(4):618-653.
  • 15. Aksu EH, Kandemir FM, Yıldırım S, et al. Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats. J Biochem Mol Toxicol. 2019;33(10):e22384.
  • 16. Aksu EH, Kandemir FM, Özkaraca M, Ömür AD, Küçükler S, Çomaklı S. Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats. Andrologia. 2017;49(1):e12593.
  • 17. Aksu EH, Kandemir FM, Altun S, Küçükler S, Çomaklı S, Ömür AD. Ameliorative effect of carvacrol on cisplatin‐Induced reproductive damage in male rats. J Biochem Mol Toxicol. 2016;30(10):513-520.
  • 18. Aksu EH, Kandemir FM, Küçükler S. Effects of hesperidin on colistininduced reproductive damage, autophagy, apoptosis by reducing oxidative stress. Andrologia. 2021;53(2):e13900.
  • 19. Ömür AD, Kandemir FM, Yıldırım BA, et al. Protective effect of dandelion (Taraxacum officinale) extract Against gentamicin-induced reproductive damage in male rats. Kafkas Üniv Vet Fak Derg. 2016;22(6):929-936.
  • 20. Sahin M, Cam H, Olgar S, et al. Protective role of erdosteine on vancomycin-induced oxidative stress in rat liver. Mol Cell Biochem. 2006;291(1-2):155-160.
  • 21. Güzel S, Şahinoğullari ZU, Canacankatan N, Antmen ŞE, Kibar D, Bayrak G. The ameliorating effect of silymarin against vancomycininduced apoptosis and inflammation in rat liver. J Res Pharm. 2019;23(4):719-728.
  • 22. Elyasi S, Khalili H, Dashti-Khavidaki S, Mohammadpour A. Vancomycin-induced nephrotoxicity: mechanism, incidence, risk factors and special populations. A literature review. Eur J Clin Pharmacol. 2012;68(9):1243-1255.

Vankomisin’in Ratlarda Sperm Motilitesi ve Testiküler Yangı Üzerine Etkileri

Year 2022, Volume: 17 Issue: 2, 41 - 44, 01.10.2022
https://doi.org/10.5152/VetSciPract.2022.221118

Abstract

Bu çalışma, erkek sıçanlarda vankomisinin sperm motilitesi ve testis yangısı üzerindeki etkisini araştırmayı amaçlamıştır. Çalışmada on iki yetişkin Sprague Dawley sıçanı kullanıldı. Ratlar iki eşit gruba ayrıldılar. Birinci grup (n = 6) kontrol grubu olarak adlandırıldı. Kontrol grubuna intraperitoneal enjeksiyon yoluyla ardışık 7 gün boyunca serum fizyolojik verildi. İkinci gruba Vankomisin grubu adı verildi ve vankomisin grubuna ardışık 7 gün boyunca intraperitoneal enjeksiyon yoluyla 200 mg/kg dozunda vankomisin verildi. Çalışmanın sonunda ratlar sevofluran anestezisi altında sakrifiye edildi. Testisler ve kauda epididimisleri toplandı. Ortalama Testis Ağırlığı (OTA), Ortalama kauda epididimis ağırlığı (OKEA) ve sperm motilitesi değerlendirildi. Ayrıca testis dokularında TNFα ve NF KB düzeyleri değerlendirildi. Sonuçlarımıza göre Vankomisin tedavisi, kontrol grubuna göre OTA ve OKEA değiştirmezken sperm motilitesini önemli ölçüde (P < ,05) azalttı. Vankomisin grubunda ise TNFα ve NF KB düzeyleri kontrol grubundan önemli ölçüde (P < ,001) yüksek bulundu. Sonuç olarak Vankomisin tedavisi (200 mg/kg vücut ağırlığı, ardışık 7 gün) sperm motilitesini önemli ölçüde azaltmakta ve testiküler inflamasyon biyobelirteçlerinin (TNFα ve NF KB) düzeylerini artırmaktadır. Vankomisinin erkek üreme sisteminde yan etkilerinden korunmak için yeni çalışmalara ihtiyaç olduğu kanaatine varılmıştır.

References

  • 1. Álvarez R, López Cortés LEL, Molina J, Cisneros JM, Pachón J. Optimizing the clinical use of vancomycin. Antimicrob Agents Chemother. 2016;60(5):2601-2609.
  • 2. Kucukler S, Darendelioğlu E, Caglayan C, Ayna A, Yıldırım S, Kandemir FM. Zingerone attenuates vancomycin-induced hepatotoxicity in rats through regulation of oxidative stress, inflammation and apoptosis. Life Sci. 2020;259:118382.
  • 3. Kandemir FM, Yildirim S, Kucukler S, Caglayan C, Mahamadu A, Dortbudak MB. Therapeutic efficacy of zingerone against vancomycin-induced oxidative stress, inflammation, apoptosis and aquaporin 1 permeability in rat kidney. Biomed Pharmacother. 2018;105:981-991.
  • 4. Naidu ECS, Olojede SO, Lawal SK, Peter AI, Akang EA, Azu OO. Effects of vancomycin linoleic acid nanoparticles on male reproductive indices of Sprague–Dawley rats. Artif Cells Nanomed Biotechnol. 2021;49(1):587-595.
  • 5. Butterfield TA, Best TM, Merrick MA. The dual roles of neutrophils and macrophages in inflammation: a critical balance between tissue damage and repair. J Athl Train. 2006;41(4):457-465.
  • 6. Zhang P, Zheng Y, Lv Y, et al. Melatonin protects the mouse testis against heat-induced damage. Mol Hum Reprod. 2020;26(2):65-79.
  • 7. Aksu EH, Akman O, Ömür AD, et al. 3, 3 diindolylmethane leads to apoptosis, decreases sperm quality, affects blood estradiol 17 β and testosterone, oestrogen (α and β) and androgen receptor levels in the reproductive system in male rats. Andrologia. 2016;48(10): 1155-1165.
  • 8. Dutta S, Sengupta P, Slama P, Roychoudhury S. Oxidative stress, testicular inflammatory pathways, and male reproduction. Int J Mol Sci. 2021;22(18):10043.
  • 9. Yang D, Elner SG, Bian ZM, Till GO, Petty HR, Elner VM. Pro-inflammatory cytokines increase reactive oxygen species through mitochondria and NADPH oxidase in cultured RPE cells. Exp Eye Res. 2007;85(4):462-472.
  • 10. Anderson MT, Staal FJ, Gitler C, Herzenberg LA, Herzenberg LA. Separation of oxidant-initiated and redox-regulated steps in the NFkappa B signal transduction pathway. Proc Natl Acad Sci U S A. 1994;91(24):11527-11531.
  • 11. Hales DB, Diemer T, Hales KH. Role of cytokines in testicular function. Endocrine. 1999;10(3):201-217.
  • 12. Feuerstein GZ, Liu T, Barone FC. Cytokines, inflammation, and brain injury: role of tumor necrosis factor-alpha. Cerebrovasc Brain Metab Rev. 1994;6(4):341-360.
  • 13. Cudicini C, Lejeune H, Gomez E, et al. Human Leydig cells and Sertoli cells are producers of interleukins-1 and -6. J Clin Endocrinol Metab. 1997;82(5):1426-1433.
  • 14. Zhang T, Ma C, Zhang Z, Zhang H, Hu H. NF-κB signalling in inflammation and cancer. MedComm. 2021;2(4):618-653.
  • 15. Aksu EH, Kandemir FM, Yıldırım S, et al. Palliative effect of curcumin on doxorubicin‐induced testicular damage in male rats. J Biochem Mol Toxicol. 2019;33(10):e22384.
  • 16. Aksu EH, Kandemir FM, Özkaraca M, Ömür AD, Küçükler S, Çomaklı S. Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats. Andrologia. 2017;49(1):e12593.
  • 17. Aksu EH, Kandemir FM, Altun S, Küçükler S, Çomaklı S, Ömür AD. Ameliorative effect of carvacrol on cisplatin‐Induced reproductive damage in male rats. J Biochem Mol Toxicol. 2016;30(10):513-520.
  • 18. Aksu EH, Kandemir FM, Küçükler S. Effects of hesperidin on colistininduced reproductive damage, autophagy, apoptosis by reducing oxidative stress. Andrologia. 2021;53(2):e13900.
  • 19. Ömür AD, Kandemir FM, Yıldırım BA, et al. Protective effect of dandelion (Taraxacum officinale) extract Against gentamicin-induced reproductive damage in male rats. Kafkas Üniv Vet Fak Derg. 2016;22(6):929-936.
  • 20. Sahin M, Cam H, Olgar S, et al. Protective role of erdosteine on vancomycin-induced oxidative stress in rat liver. Mol Cell Biochem. 2006;291(1-2):155-160.
  • 21. Güzel S, Şahinoğullari ZU, Canacankatan N, Antmen ŞE, Kibar D, Bayrak G. The ameliorating effect of silymarin against vancomycininduced apoptosis and inflammation in rat liver. J Res Pharm. 2019;23(4):719-728.
  • 22. Elyasi S, Khalili H, Dashti-Khavidaki S, Mohammadpour A. Vancomycin-induced nephrotoxicity: mechanism, incidence, risk factors and special populations. A literature review. Eur J Clin Pharmacol. 2012;68(9):1243-1255.
There are 22 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Özge Kandemir This is me 0000-0001-8884-4168

Emrah Hicazi Aksu This is me 0000-0003-1591-684X

Publication Date October 1, 2022
Published in Issue Year 2022 Volume: 17 Issue: 2

Cite

APA Kandemir, Ö., & Aksu, E. H. (2022). The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats. Veterinary Sciences and Practices, 17(2), 41-44. https://doi.org/10.5152/VetSciPract.2022.221118
AMA Kandemir Ö, Aksu EH. The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats. Veterinary Sciences and Practices. October 2022;17(2):41-44. doi:10.5152/VetSciPract.2022.221118
Chicago Kandemir, Özge, and Emrah Hicazi Aksu. “The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats”. Veterinary Sciences and Practices 17, no. 2 (October 2022): 41-44. https://doi.org/10.5152/VetSciPract.2022.221118.
EndNote Kandemir Ö, Aksu EH (October 1, 2022) The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats. Veterinary Sciences and Practices 17 2 41–44.
IEEE Ö. Kandemir and E. H. Aksu, “The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats”, Veterinary Sciences and Practices, vol. 17, no. 2, pp. 41–44, 2022, doi: 10.5152/VetSciPract.2022.221118.
ISNAD Kandemir, Özge - Aksu, Emrah Hicazi. “The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats”. Veterinary Sciences and Practices 17/2 (October 2022), 41-44. https://doi.org/10.5152/VetSciPract.2022.221118.
JAMA Kandemir Ö, Aksu EH. The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats. Veterinary Sciences and Practices. 2022;17:41–44.
MLA Kandemir, Özge and Emrah Hicazi Aksu. “The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats”. Veterinary Sciences and Practices, vol. 17, no. 2, 2022, pp. 41-44, doi:10.5152/VetSciPract.2022.221118.
Vancouver Kandemir Ö, Aksu EH. The Effects of Vancomycin on Sperm Motility and Testicular Inflammation in Rats. Veterinary Sciences and Practices. 2022;17(2):41-4.

Content of this journal is licensed under a Creative Commons Attribution NonCommercial 4.0 International License

2987230564