Investigation of the Effectiveness of Chromogenic Media in the Isolation of Pasteurella multocida and Mannheimia haemolytica from Calf Nasal Swab Samples

Year 2022, Volume: 17 Issue: 3, 81 - 86, 06.01.2023

Osman Yaşar Tel Songül Ötkün Ayfer Güllü Yücetepe Oktay Keskin

https://doi.org/10.5152/VetSciPract.2022.221830

Abstract

Accurate and rapid diagnosis of disease agents is the most important step in terms of control
practices. Chromogenic media are culture media that allow the formation of colonies in colors
specific to target microorganisms. Because they are target-specific, they do not require validation
of results but provide ease of use and time savings. In this study, it was aimed to evaluate
the effectiveness of a chromogenic medium (Pasteurella BDR kit) in the detection of Pasteurella
multocida and Mannheimia haemolytica agents in the Pasteurelleceae family, which cause respiratory
disease in cattle. In this study, nasal swab samples taken from calves showing symptoms
of respiratory disease were cultured in chromogenic and standard media. Suspicious growing
colonies were confirmed by polymerase chain reaction for P. multocida and M. haemolytica. While
31 (36.9%) samples formed colonies with the chromogenic medium in the color specific to the
target bacteria, 28 (33.3%) samples were positively determined for the target bacteria using the
standard cultural method. The results of 26 samples were positive by both cultural diagnosis
methods. When the results were compared with the traditional cultural diagnosis, agreement
was found to be 92.86%. All colored colonies grown on the chromogenic medium were also tested
by polymerase chain reaction (PCR). It was determined that the chromogenic medium detected P.
multocida at a rate of 92.86% (n = 26) and M. haemolytica at a rate of 100% (n = 2) by forming colonies
with a family-specific color. As a result, it was concluded that the use of chromogenic media
is beneficial in the practical, rapid, and high-accuracy diagnosis of target agents.

Keywords

Kromojenik besiyeri, Pasteurella, sığır solunum hastalığı, kültür

Buzağı Nasal Svap Örneklerinden Pasteurella multocida ve Mannheimia haemolytica İzolasyonunda Kromojenik Besiyerinin Etkinliğinin Araştırılması

Abstract

Hastalık etkenlerinin doğru ve hızlı teşhisi, kontrol uygulamaları açısından en önemli basamaktır.
Kromojenik besiyerleri hedef mikroorganizmalara özgü renkte kolonilerin meydana gelmesini
sağlayan kültür ortamlarıdır. Bunlar hedefe özgü olduklarından sonuçların doğrulama ihtiyacı duymamakla
birlikte, kullanım kolaylığı ve zaman tasarrufu sağlarlar. Bu çalışmada, sığırlarda solumun
hastalığına neden olan Pasteurelleceae ailesinde bulunan Pasteurella multocida ve Mannheimia
haemolytica etkenlerinin tespitinde, kromojenik besiyerinin (Pasteurella BRD Kit) etkinliğini
değerlendirmek amaçlandı. Çalışmada solunum hastalığı belirtisi gösteren buzağılardan alınan
nasal svap örnekleri kromojenik ve standart besiyerlerinde kültüre edildi. Üreyen şüpheli koloniler
Pasteurella multocida ve Mannheimia haemolytica yönünden Polimeraz Zincir Reaksiyonu
(PCR) ile doğrulandı. Kromojenik besiyeri ile 31 (%36,9) numune, hedef bakterilere özgü renkte
koloni oluştururken, standard kültürel yöntem ile 28 (%33,3) numune hedef bakteriler yönünden
pozitif olarak belirlendi. Her iki kültürel tanı yöntemiyle 26 numunenin sonuçları pozitif olarak tespit
edildi. Sonuçlar geleneksel kültürel tanı ile karşılaştırıldığında %92,86 oranında uyumlu sonuç bulundu. Kromojenik besiyerinde üreyen renkli kolonilerin tamamı PCR ile de test edildi. Kromojenik besiyeri P. multocida’yı %92,86
(n:26), M. haemolytica’yı %100 (n:2) oranında aileye özgü renkte koloni meydana getirme suretiyle tespit ettiği belirlendi.
Sonuç olarak, hedef etkenin pratik, hızlı ve yüksek doğrulukla teşhisinde kromojenik besiyerinin kullanımının yararlı olduğu kanısına
varıldı.

Keywords

Kromojenik besiyeri, Pasteurella, sığır solunum hastalığı, kültür

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