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Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats

Year 2023, Volume: 18 Issue: 3, 90 - 94, 20.12.2023

Abstract

The aim of the present study was to determine the effects of Vitis vinifera L. seed extract against reproductive damage induced by thallium sulfate in rats. For this purpose, a total of 24 male rats, 6 rats in each group, were used for the study. In the control group, the rats were given 0.5% carboxymethyl cellulose followed by physiological saline. In the thallium sulfate group, the rats were given thallium sulfate (10 mg/kg) by intraperitoneal injection. In thallium sulfate + Vitis vinifera L. extract groups,The rats were administered thallium sulfate (10 mg/kg/intraperitoneal) + Vitis vinifera L. extract (100 mg/kg) peros. In Vitis vinifera L. extract group, the rats were given Vitis vinifera L. extract (100 mg/kg) peros. After the experimental process was finished, the rats were sacrificed and the blood and testicular tissues were taken. The results of our study found that the malondialdehyde level in the blood and testicular tissues of the thallium sulfate group was statistically higher than the other experimental groups (P < .05). Catalase, glutathione peroxidase activity, and glutathione level were the lowest in the thallium sulfate group (P < .05). In the histopathological examination, necrotic and degenerative findings are seen in the thallium sulfate group. Cleaved caspase-3 was strongly expressed in seminiferous tubules in the thallium sulfate group, but cleaved caspase-3 level was inhibited in thallium sulfate + Vitis vinifera L. extract group. Total motility value was found to be lower in the thallium sulfate group compared to the other experimental groups (P < .05). As a result, the biochemical, histopathological, and spermatological effects of Vitis vinifera L. extract administered as a peros against the deteriorated physiological functions in rats given thallium sulfate were determined.

References

  • 1. D’Orazio M, Campanella B, Bramanti E, et al. Thallium pollution in water, soils and plants from a past-mining site of Tuscany: sources, transfer processes and toxicity. J Geochem Explor. 2020;209:106434. [CrossRef]
  • 2. Peter AJ, Viraraghavan T. Thallium: a review of public health and environmental concerns. Environment international. 2005;31(4):493-501.
  • 3. Heim M, Wappelhorst O, Markert B. Thallium in terrestrial environmen ts–oc curre nce and effects. Ecotoxicology. 2002;11(5):369- 377. [CrossRef]
  • 4. Li B, Zhang J, Ma J, et al. The continuous physiological changes of zebrafish (Danio rerio) based on metabolism under controlled thallium stress. Chemosphere. 2020;240:124974. [CrossRef]
  • 5. Chia CF, Chen SC, Chen CS, Shih CM, Lee HM, Wu CH. Thallium acetate induces C6 glioma cell apoptosis. Ann N Y Acad Sci. 2005;1042(1):523-530. [CrossRef]
  • 6. Osorio-Rico L, Santamaria A, Galván-Arzate S. Thallium toxicity: general issues, neurological symptoms, and neurotoxic mechanisms. Adv Neurobiol. 2017;18:345-353. [CrossRef]
  • 7. Abu-Serie MM, Habashy NH. Vitis vinifera polyphenols from seedless black fruit act synergistically to suppress hepatotoxicity by targeting necroptosis and pro-fibrotic mediators. Sci Rep. 2020;10(1):2452. [CrossRef]
  • 8. Felicio J, Santos RdS, Gonçalez E. Chemical constituents from Vitis vinifera (Vitaceae). Arq Inst Biol. 2001;68(1):47-50.
  • 9. Iacopini P, Baldi M, Storchi P, Sebastiani L. Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: content, in vitro antioxidant activity and interactions. J Food Compos Anal. 2008;21(8): 589-598. [CrossRef]
  • 10. Alawi N, Hag G, Rabah S, et al. The protective effect of grape (Vitis vinifera) seed oil on testicular structure of male rats exposed to lead. Adv Biol Res. 2018;12(1):16-25.
  • 11. Zhao YM, Gao LP, Zhang HL, Guo JX, Guo PP. Grape seed proanthocyanidin extract prevents DDP-induced testicular toxicity in rats. Food Funct. 2014;5(3):605-611. [CrossRef]
  • 12. Kordali S, Cakir A, Akcin TA, et al. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola HubMor. and Achillea biebersteinii Afan. (Asteraceae). Ind Crops Prod. 2009;29(2-3):562-570. [CrossRef]
  • 13. Aksu EH, Kandemir FM, Küçükler S. Ameliorative effect of hesperidin on strep tozot ocin- diabe tes mellitus-induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats. J Food Biochem. 2021;45(10):e13938. [CrossRef]
  • 14. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979; 135(3):372-376. [CrossRef]
  • 15. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969;27(3):502-522. [CrossRef]
  • 16. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991;196(2-3):143-151. [CrossRef]
  • 17. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500. [CrossRef]
  • 18. Matkovics B. Determination of enzyme activity in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika. 1988;15: 248-250.
  • 19. Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem. 1966;16(2):359-364. [CrossRef]
  • 20. Fernández V, Videla LA. Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of various tissues of the rat. Experientia. 1981;37(4):392-394. [CrossRef]
  • 21. Ömur AD, Yildirim B, Saglam YS, Comakli S, Ozkaraca M. Activity of resveratrol on the influence of aflatoxin b1 on the testes of Sprague Dawley rats. Pol J Vet Sci. 2019;22(2):313-320. [CrossRef]
  • 22. Sevim Ç, Çomaklı S, Taghizadehghalehjoughi A, et al. An imazamoxbased herbicide causes apoptotic changes in rat liver and pancreas. Toxicol Rep. 2019;6:42-50. [CrossRef]
  • 23. Gur C, Akarsu SA, Akaras N, Tuncer SC, Kandemir FM. Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environ Toxicol. 2023;38(6). [CrossRef]
  • 24. Akarsu SA, Türk G, Arkalı G, Çeribaşı AO, Yüce A. Changes in heavy metal levels, reproductive characteristics, oxidative stress markers and testicular apoptosis in rams raised around thermal power plant. Theriogenology. 2022;179:211-222. [CrossRef]
  • 25. Bhardwaj JK, Paliwal A, Saraf P. Effects of heavy metals on reproduction owing to infertility. J Biochem Mol Toxicol. 2021;35(8):e22823. [CrossRef]
  • 26. Riyaz R, Pandalai SL, Schwartz M, Kazzi ZN. A fatal case of thallium toxicity: challenges in management. J Med Toxicol. 2013;9(1):75-78. [CrossRef]
  • 27. Formigli L, Scelsi R, Poggi P, et al. Thallium-induced testicular toxicity in the rat. Environ Res. 1986;40(2):531-539. [CrossRef]
  • 28. Nuray K, Karadeniz F. Gıdalardaki doğal antioksidan bileşikler. 2014 11(1).25-30
  • 29. Rajesh Kumar TR, Doreswamy K, Shrilatha B, Muralidhara. Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutat Res Genet Toxicol Environ Mutagen. 2002;513(1-2):103-111. [CrossRef]
  • 30. Renehan AG, Booth C, Potten CS. What is apoptosis, and why is it important? BMJ. 2001;322(7301):1536-1538. [CrossRef]
  • 31. Lee J, Richburg JH, Shipp EB, Meistrich ML, Boekelheide K. The Fas system, a regulator of testicular germ cell apoptosis, is differentially up-regulated in Sertoli cell versus germ cell injury of the testis. Endocrinology. 1999;140(2):852-858. [CrossRef]
  • 32. Strasser A, O’Connor L, Dixit VM. Apoptosis Signaling. Annu Rev Biochem. 2000;69(1):217-245. [CrossRef]
  • 33. Overbeeke R, Steffens-Nakken H, Vermes I, Reutelingsperger C, Haanen C. Early features of apoptosis detected by four different flow cytometry assays. Apoptosis. 1998;3(2):115-121. [CrossRef]
  • 34. Korsmeyer SJ. Regulators of cell death. Trends Genet. 1995;11(3): 101-105. [CrossRef]

Erkek Sıçanlarda Talyum (I) Sülfatın Neden Olduğu Üreme Hasarına Karşı Vitis vinifera L. cv “Merlot” Tohum Ekstraktının Etkileri

Year 2023, Volume: 18 Issue: 3, 90 - 94, 20.12.2023

Abstract

Bu çalışmanın amacı sıçanlarda talyum sülfatın neden olduğu üreme hasarına karşı Vitis vinifera L. tohum ekstraktının etkilerini belirlemektir. Bu amaçla çalışmada her grupta 6 adet olmak üzere toplam 24 adet erkek sıçan kullanıldı. Kontrol grubundaki sıçanlara %0,5 karboksimetil selüloz ve ardından serumm fizyolojik verildi. Talyum sülfat grubundaki sıçanlara intraperitoneal enjeksiyon yoluyla talyum sülfat (10 mg/kg) verildi. Talyum sülfat + Vitis vinifera L. ekstresi gruplarında sıçanlara talyum sülfat (10 mg/kg /intr aperi tonea l)+Vi tis vinifera L. ekstraktı (100 mg/kg) peros uygulama yapıldı. Vitis vinifera L. ekstresi grubundaki sıçanlara Vitis vinifera L. ekstresi (100 mg/ kg) peros verildi. Sonra deney süreci tamamlanarak sıçanlar sakrifiye edildi ve kan ve testis dokuları alındı. Çalışmamızın sonuçlarına göre kan ve testislerdeki malondialdehit düzeyinin Talyum sülfat grubunda diğer deney gruplarına göre istatistiksel olarak daha yüksekti (P < .05). Talyum sülfat grubunda katalaz, glutatyon peroksidaz aktivitesi ve glutatyon düzeyi en düşük seviyedeydi(P < .05). Histopatolojik incelemede talyum sülfat grubunda nekrotik ve dejeneratif bulgular görülmektedir. Talyum sülfat grubunda kaspaz-3 güçlü bir şekilde ifade edilirken talyum sülfat+- Vitis vinifera L. ekstrakt grubunda kaspaz-3 seviyesi inhibe edildi. Talyum sülfat grubunda total motilite değerinin diğer deney grupları ile karşılaştırıldığında daha düşük olduğu görüldü (P < .05). Sonuç olarak sıçanlarda Talyum sülfat tarafından bozulan fizyolojik fonksiyonlara karşı peros olarak uygulanan Vitis vinifera L. ekstraktı’nın biyokimyasal, histopatolojik ve spermatolojik iyileştirici etkileri olduğu belirlendi.

References

  • 1. D’Orazio M, Campanella B, Bramanti E, et al. Thallium pollution in water, soils and plants from a past-mining site of Tuscany: sources, transfer processes and toxicity. J Geochem Explor. 2020;209:106434. [CrossRef]
  • 2. Peter AJ, Viraraghavan T. Thallium: a review of public health and environmental concerns. Environment international. 2005;31(4):493-501.
  • 3. Heim M, Wappelhorst O, Markert B. Thallium in terrestrial environmen ts–oc curre nce and effects. Ecotoxicology. 2002;11(5):369- 377. [CrossRef]
  • 4. Li B, Zhang J, Ma J, et al. The continuous physiological changes of zebrafish (Danio rerio) based on metabolism under controlled thallium stress. Chemosphere. 2020;240:124974. [CrossRef]
  • 5. Chia CF, Chen SC, Chen CS, Shih CM, Lee HM, Wu CH. Thallium acetate induces C6 glioma cell apoptosis. Ann N Y Acad Sci. 2005;1042(1):523-530. [CrossRef]
  • 6. Osorio-Rico L, Santamaria A, Galván-Arzate S. Thallium toxicity: general issues, neurological symptoms, and neurotoxic mechanisms. Adv Neurobiol. 2017;18:345-353. [CrossRef]
  • 7. Abu-Serie MM, Habashy NH. Vitis vinifera polyphenols from seedless black fruit act synergistically to suppress hepatotoxicity by targeting necroptosis and pro-fibrotic mediators. Sci Rep. 2020;10(1):2452. [CrossRef]
  • 8. Felicio J, Santos RdS, Gonçalez E. Chemical constituents from Vitis vinifera (Vitaceae). Arq Inst Biol. 2001;68(1):47-50.
  • 9. Iacopini P, Baldi M, Storchi P, Sebastiani L. Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: content, in vitro antioxidant activity and interactions. J Food Compos Anal. 2008;21(8): 589-598. [CrossRef]
  • 10. Alawi N, Hag G, Rabah S, et al. The protective effect of grape (Vitis vinifera) seed oil on testicular structure of male rats exposed to lead. Adv Biol Res. 2018;12(1):16-25.
  • 11. Zhao YM, Gao LP, Zhang HL, Guo JX, Guo PP. Grape seed proanthocyanidin extract prevents DDP-induced testicular toxicity in rats. Food Funct. 2014;5(3):605-611. [CrossRef]
  • 12. Kordali S, Cakir A, Akcin TA, et al. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola HubMor. and Achillea biebersteinii Afan. (Asteraceae). Ind Crops Prod. 2009;29(2-3):562-570. [CrossRef]
  • 13. Aksu EH, Kandemir FM, Küçükler S. Ameliorative effect of hesperidin on strep tozot ocin- diabe tes mellitus-induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats. J Food Biochem. 2021;45(10):e13938. [CrossRef]
  • 14. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979; 135(3):372-376. [CrossRef]
  • 15. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969;27(3):502-522. [CrossRef]
  • 16. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991;196(2-3):143-151. [CrossRef]
  • 17. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500. [CrossRef]
  • 18. Matkovics B. Determination of enzyme activity in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika. 1988;15: 248-250.
  • 19. Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem. 1966;16(2):359-364. [CrossRef]
  • 20. Fernández V, Videla LA. Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of various tissues of the rat. Experientia. 1981;37(4):392-394. [CrossRef]
  • 21. Ömur AD, Yildirim B, Saglam YS, Comakli S, Ozkaraca M. Activity of resveratrol on the influence of aflatoxin b1 on the testes of Sprague Dawley rats. Pol J Vet Sci. 2019;22(2):313-320. [CrossRef]
  • 22. Sevim Ç, Çomaklı S, Taghizadehghalehjoughi A, et al. An imazamoxbased herbicide causes apoptotic changes in rat liver and pancreas. Toxicol Rep. 2019;6:42-50. [CrossRef]
  • 23. Gur C, Akarsu SA, Akaras N, Tuncer SC, Kandemir FM. Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite-induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environ Toxicol. 2023;38(6). [CrossRef]
  • 24. Akarsu SA, Türk G, Arkalı G, Çeribaşı AO, Yüce A. Changes in heavy metal levels, reproductive characteristics, oxidative stress markers and testicular apoptosis in rams raised around thermal power plant. Theriogenology. 2022;179:211-222. [CrossRef]
  • 25. Bhardwaj JK, Paliwal A, Saraf P. Effects of heavy metals on reproduction owing to infertility. J Biochem Mol Toxicol. 2021;35(8):e22823. [CrossRef]
  • 26. Riyaz R, Pandalai SL, Schwartz M, Kazzi ZN. A fatal case of thallium toxicity: challenges in management. J Med Toxicol. 2013;9(1):75-78. [CrossRef]
  • 27. Formigli L, Scelsi R, Poggi P, et al. Thallium-induced testicular toxicity in the rat. Environ Res. 1986;40(2):531-539. [CrossRef]
  • 28. Nuray K, Karadeniz F. Gıdalardaki doğal antioksidan bileşikler. 2014 11(1).25-30
  • 29. Rajesh Kumar TR, Doreswamy K, Shrilatha B, Muralidhara. Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutat Res Genet Toxicol Environ Mutagen. 2002;513(1-2):103-111. [CrossRef]
  • 30. Renehan AG, Booth C, Potten CS. What is apoptosis, and why is it important? BMJ. 2001;322(7301):1536-1538. [CrossRef]
  • 31. Lee J, Richburg JH, Shipp EB, Meistrich ML, Boekelheide K. The Fas system, a regulator of testicular germ cell apoptosis, is differentially up-regulated in Sertoli cell versus germ cell injury of the testis. Endocrinology. 1999;140(2):852-858. [CrossRef]
  • 32. Strasser A, O’Connor L, Dixit VM. Apoptosis Signaling. Annu Rev Biochem. 2000;69(1):217-245. [CrossRef]
  • 33. Overbeeke R, Steffens-Nakken H, Vermes I, Reutelingsperger C, Haanen C. Early features of apoptosis detected by four different flow cytometry assays. Apoptosis. 1998;3(2):115-121. [CrossRef]
  • 34. Korsmeyer SJ. Regulators of cell death. Trends Genet. 1995;11(3): 101-105. [CrossRef]

Details

Primary Language English
Subjects Veterinary Sciences (Other)
Journal Section Research Articles
Authors

Serkan Ali AKARSU This is me

Publication Date December 20, 2023
Published in Issue Year 2023 Volume: 18 Issue: 3

Cite

APA AKARSU, S. A. (2023). Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats. Veterinary Sciences and Practices, 18(3), 90-94.
AMA AKARSU SA. Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats. Veterinary Sciences and Practices. December 2023;18(3):90-94.
Chicago AKARSU, Serkan Ali. “Effects of Vitis Vinifera L. Cv. ‘Merlot’ Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats”. Veterinary Sciences and Practices 18, no. 3 (December 2023): 90-94.
EndNote AKARSU SA (December 1, 2023) Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats. Veterinary Sciences and Practices 18 3 90–94.
IEEE S. A. AKARSU, “Effects of Vitis vinifera L. cv. ‘Merlot’ Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats”, Veterinary Sciences and Practices, vol. 18, no. 3, pp. 90–94, 2023.
ISNAD AKARSU, Serkan Ali. “Effects of Vitis Vinifera L. Cv. ‘Merlot’ Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats”. Veterinary Sciences and Practices 18/3 (December 2023), 90-94.
JAMA AKARSU SA. Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats. Veterinary Sciences and Practices. 2023;18:90–94.
MLA AKARSU, Serkan Ali. “Effects of Vitis Vinifera L. Cv. ‘Merlot’ Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats”. Veterinary Sciences and Practices, vol. 18, no. 3, 2023, pp. 90-94.
Vancouver AKARSU SA. Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats. Veterinary Sciences and Practices. 2023;18(3):90-4.

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