Ratlarda finasterid ve dutasteridin prostat vaskülaritesine etkisinin araştırılması

Year 2014, Volume: 9 Issue: 1, 55 - 62, 01.01.2014

Engin Doğantekin Emre Tüzel

Abstract

Amaç: Finasterid ve dutasterid verilen ratların prostatlarında özellikle vasküler olmak üzere histopatolojik değişikliklerin araştırılması. Gereç ve Yöntemler: Çalışma grubundaki 20 adet Wistar Albino suşu rata günlük 80 mg/kg/gün finasterid n=10 veya 16 mg/kg/gün dutasterid n=10 , 15 gün boyunca orogastrik gavaj enjektörü ile verildi. Kontrol grubundaki 6 rata ilaç verilmedi. Deney sonunda ratlar anestezi ile sakrifiye edildikten sonra, testisleri, veziküla seminalisleri ve prostatları cerrahi olarak eksize edildi. Prostat kesitleri, endoteli işaretlemek için CD31 antikoru ile immünohistokimyasal olarak boyandı ve ışık mikroskobu altında incelendi. Ortalama mikrodamar yoğunluğu MDY , ortalama proliferatif mikrodamar yoğunluğu PMDY , ortalama damar alanı DAL ve ortalama damar çevresi uzunluğu DÇEV , 3 farklı alanda X200 büyütmede, dijital görüntüleme sistemi kullanılarak hesaplandı.Bulgular: Çalışma sonunda kontrol, finasterid ve dutasterid gruplarında prostat ağırlığı sırasıyla; 0.47±0.02 gr, 0.28±0.02 gr ve 0.19±0.02 gr olarak tespit edildi p=0.006 . Kontrol, finasterid ve dutasterid gruplarında MDY değerleri sırasıyla; 5.16, 11.66 ve 10.28 olarak bulundu p=0.001 . Kontrol, finasterid ve dutasterid gruplarında PMDY sırasıyla; 6.33, 15.23 ve 15.43 olarak saptandı p=0.001 . Dunificantly lower in dutasterid group compared to finasteride group 341.71 vs. 814.71 µm2, p=0.019 and 69.51 vs. 109.34 µm, p=0.007, respectively .Conclusion: In the dutasteride group there was a statistically significant decrease in the weight of the prostate gland at the end of study compared to the other groups. The MVD was significantly higher in dutasteride and finasteride group compared to control group, but not different between finasteride and dutasteride group.tasterid grubunda DAL ve DÇEV değerleri, finasterid grubuna göre anlamlı ölçüde düşük olarak bulundu 341.71’e karşı 814.71 µm2, p=0.019 ve 69.51’e karşı 109.34 µm, p=0.007 .Sonuçlar: Dutasterid grubunda, deney sonrası prostat ağırlığında diğer gruplara göre istatistiksel olarak anlamlı azalma mevcuttu. Dutasterid ve finasterid gruplarında MDY kontrol grubuna göre anlamlı ölçüde yüksek olduğu, fakat finasterid ve dutasterid verilen ratlarda farksız olduğu saptandı

Keywords

Finasterid , Dutasterid

To investigate the effect of finasteride and dutasteride on rats prostate vascularity

Abstract

Objective: To investigate the histopathological changes, particularly vascular changes on prostate glands of the rats that were treated with finasteride and dutasteride. Material and Methods: Either finasteride 80 mg/kg/day n=10 or dutasteride 16 mg/kg/ day n=10 for 15 days were administered to 20 Wistar Albino rats dailly in study group with orogastric gavage syringe. No drugs were administered to the control group n=6 . At the end of the study, all rats were anesthetically sacrified and their testicles, seminal vesicles and prostates were excised surgically. Prostatic sections were stained with CD31 antibodies immunohistochemically to mark endothelium and were examined under light microscopy. The mean microvessel dansity MVD , mean proliferative MVD PMVD , mean vessel area MVA and the mean length of vessel circumference MVCD were measured in three different areas on X200 magnification by using digital imaging system. Results: The weight of the prostate at the end of the study in control, finasterid and dutasterid group was; 0.47±0.02 gr, 0.28±0.02 gr and 0.19±0.02 gr, respectively p=0.006 . The MVD in control, finasterid and dutasterid group were 5.16, 11.66 and 10.28, respectively p=0.001 . The PMVD in control, finasterid and dutasterid group was 6.33, 15.23 and 15.43, respectively p=0.001 . The MVA and the MVCD were significantly lower in dutasterid group compared to finasteride group 341.71 vs. 814.71 µm2 , p=0.019 and 69.51 vs. 109.34 µm, p=0.007, respectively . Conclusion: In the dutasteride group there was a statistically significant decrease in the weight of the prostate gland at the end of study compared to the other groups. The MVD was significantly higher in dutasteride and finasteride group compared to control group, but not different between finasteride and dutasteride group

Keywords

Finasteride , Dutasteride

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