Köpeklerde deneysel nefrotoksikozisde eritropoietin seviyesi ve bazı hematolojik parametreler üzerinde araştırmalar

Year 2000, Volume: 6 Issue: 1-2 - 2000, 64 - 71, 18.06.2000

Ebubekir Ceylan Zahid T. Ağaoğlu

Abstract

Bu çalışmada, köpeklerde deneysel oluşturulan nelfotoksikoziste Epo seviyesi ve bazı kan parametreleri değerlendirilerek nefritislerdeki etkinliği araştırıldı. Bu çalışmanın materyalini 18 adet sağlıklı köpek oluşturdu. Gentamisin enjeksiyonları 10 gün süreyle günde 3 kez saat: 8.00, 12.00, 24.00 olmak üzere A grubuna n=6 7 mg/kg, B grubuna n=6 10 mg/kg, C grubuna n=6 ise 15 mg/kg dozda, intramüsküler yolla yapıldı. Köpekler, uygulama öncesi ve uygulama süresince her gün klinik, 2, 5, 7 ve 12. günlerde hematolojik ve biyokimyasal muayenelerden geçirildi. A grubunda lökosit ve idrar GGT’sindeki artış p0.05 . B grubundaki hematolojik parametrelerden Iökositlerdeki artış ve MCV değerlerinde azalma; biyokimyasal parametrelerden Epo, potasyum, fosfor, kan üre nitrojen ve idrar GGT’sindeki artış; sodyum, kalsiyum, albumin ve protein değerlerindeki düşüş istatistik olarak anlamlı bulundu p

Keywords

Köpek, Eritropoietin, Böbrek, Nefrotoksikozis, Gentamisin

Investigation on erytlıropoietin level and some blood parameters in experinıental nephrotoxicosis in dogs

Abstract

İn this study, it was investigated Erythropoietin levels and some hematologic parameters in experimental nephrotoxicosis in dogs. Eighteen healhty dogs were used as a material in this study. Gentamicine sulphate was given to induce nephrotoxicity at the dose of 7 mg/kg body weight to group A, 10 mg/kg body weight to group B and 15 mg/kg body weight to group C for 10 consecutive days. Dogs were examined before the experiment and during the experiment every day clinically, 2, 5, 7 and 12. days hematologic and biochemical examinations. In group A, except inerease in leukosite and urine GGT values p0.05 . From hematologic parameters in group B, inerease in leukosite, decrease in MCV values; from the biochemical parameters inerease in Epo, potassium, phosphorus, blood urea nitrogen and urine GGT values; decrease in sodium, calcium, albumin and protein values were found statistically signifıcant p

Keywords

Dog, Erythropoietin, Kidney, Nephrotoxicosis, Gentamisin sulphate.

References

• 1. Woodman DD: Erythropoietin, Comparative Haematology International, 2: 1-7,(1992).
• 2. Fried W, Barone-Varelas J, Morley C: Factors that regulate extraıenal eıythropoietin pıoduction. Blood Cells 10: 287, (1984).
• 3. Sawyer ST: Receptors for eıythropoietin. Distribution, structuıe, and role in receptor-mediated endocytosis in eıythroid cells, in Harris JR (ed): Blood Celi Biochemistry, vol I. New York, NY, Plenıım, p365, (1990).
• 4. Spivak JL: Recombinant human erythropoietin and the anemia of cancer. Blood 84 (4): 997-1004, (1994).
• 5. Goldvvasser E. et al.: The effect of interleukin-3 on hemapoietic pıecursor cells. In: Normal and neoplastic hematopoiesis. pp. 301- 309. Alan R. Liss, New York, (1983).
• 6. Eckaıdt KIJ, Kıırtz A, Hiıth P, Scigalla P. Wieczorek L, Brauer C: Evaluation of the stability of human erythropoietin in samples for radioimmunoassay. Klin. Wochenschr., 66: 241- 245,(1989).
• 7. Caro J, Erslev AJ: Erythropoietin assays and their use in the study of anemias. Contrib. Nephrol. 66: 54, (1988).
• 8. Gaıcia J, Sherwood J, Goldvvasser E: Radioimmunoassay of erythropoietin. Blood Cells. 5: 405-419, (1979).
• 9. Dunn CDR. Lange RD: Erythıopoictin:assay and characteıization; in Roaths, Topical ıevievvs in hematology, pp:l-32 (Wright, Bristol), (1979).
• 10. Miyake T, Kung CKH, Goldwasser E: Puıifıcation of human erythropoietin. J. Biol. Chem. 252: 5558-5564, (1977).
• 11. Radtke HW, Eıbes PM, Schippers E, Koch KM: Serum erythropoietin concentration in anephric patients Nephıon. 22: 361- 365, (1978).
• 12. Spivak JL, Watson AJ: Hematopoiesis and the Kidney The Kidney: Physiology and Patophysiology. Second Ed. Raven Press, Ltd., Nevv York, Chapter 42: 1553-1593, (1992).
• 13. Fisher JW: Mechanism of the anemia of chronic renal failure, Nephron, 25; 106-11 1, (1980).
• 14. Burrows GE: Aminocyclitol antibiotics. JAVMA, 176: 1280- 1281, (1980).
• 15. Poıter GA, Bcnnett WM: Nephrotoxic acule renal failure due to common drugs. Am. J. Physiol., 241: F1-F8, (1981).
• 16. Bennett WM, Luft F, Porter GA: Pathogenesis of renal failure due to aminoglicosides and contrast media used in roentgenogıaphy. Am. J. Med., 69: 767-774, (1980).
• 17. Maden M: Deneysel gentamisin nefrotoksisitesinde üriner enzim aktivitelerinin önemi. TC S.Ü: Sağ. Bil. Enst., Doktora tezi, Konya, (1994).
• 18. Fadel AA, Larkın HA: Gentamicin-induced nephrotoxicosis in lambs. Research in veterinary Science 61: 187-192, (1996).
• 19. Haddad LM, Winchester JF: Clinical management of poisoning and drug overdose. 2nd ed. WB Saunders company. pp. 167-184, (1990).
• 20. Conzelman GM: Pharmacotherapeutics of aminoglycoside antibiotics. JAVMA, 176 (10): 1078-1080,(1980).
• 21. Berne RM, Levy MN: Physiology, 3. ed. Mosby Year Book. pp.719-784, (1993).
• 22. Canine Medicine and Therapeutics. 3. ed. In: Chandler E.A., Thompson D.J., Sutton J.B.. Prıice C.J.. Blackvvell-Science pp. 601-658, (1995).
• 23. Hinchliff KW, Shaftoe S, Dubielzing RR: Gentamicin-induced nephrotoksikozis in a cow. JAVMA 192(7): 923-925, (1988).
• 24. Coles EH: Veterinary Clinical Pathology 4lh ed., W.B. Saunders Company, Philadelphia, London, (1986).
• 25. Grauer GF, Greco DS, Behrend EN, Fettmen MJ. Jaenke RS, Ailen TA: Effects of dietary protein conditioning on gentamicin-induced nephrotoxicosis in healty male dogs. Am. J. Vet. Res., 55(1): 90- 97,(1994).
• 26. Greco DS, Turnvvald GH. Adams R, Gossett KA, Kearney M, Casey H: Urinary %-glutamyl tıanspeptidase activity in dogs with gentamicin-induced nephrotoxicity. Am. J. Res. 46(11): 2332- 2335,(1985).
• 27. Uechi M, Temi H, Nakayama T. Mishina M, Wakao Y, Takahashi M: Circadian variation of urinary enzymes in the dog. J. Vet. Med. Sci., 56(5): 849-854, (1994).
• 28. Davis LE: Gentamicin JAVMA, 175(3): 301-302, (1979).
• 29. Naughton BA, Birnbach DL, Lıu P et al.. Erythropoietin production and kupffer celi alterations following nephrectomy, hypoxia, or combined nephrectomy and hypoxia. Proc. Soc. Exp. Biol. Med. 160: 170-174, (1979).
• 30. Lacombe C, Da Silva JL, Bruneval P, Fournier JG, Wendling F, Casedevall N, Camilleri JP, Baıiety J, Varet B, Tambourin P: Peıitubuler cells are the site of erythropoietin synthesis in the murine hypoxic kidney J Clin. Invest. 81:620-623, (1988).
• 31. Eckaıdt KU, Kuıtz A, Bauer C: Regulation of erythropoietin formation is ıelated to proximal tubular function. Am J Physiol. 256; F942, (1989).
• 32. Giger U: Erythropoietin and its Clinical Use, Comp. Continuing Education Aıticle, 14(1): 25-34, (1992).
• 33. Golde DW. Hocking WG, Koeffler HP, Adamson JW: Polycythemia: Mechanisms and Management. Ann. Inter. Med., 95:71-87,(1981).
• 34. Cotes PM. Bangham DR: Bio-assay of erythropoietin in mice made polycythemic by exposure to air at a reduced pressure. Nature. 191: 1065-1067, (1961).
• 35. Dunn CD. Jarvis JH. Gıeenman JM: A quantitative bioassay for erythropoietin using moııse fetal liver cells. Exp. Hematol. 3: 65- 78,(1975).
• 36. Ikeda T, Inaba M, Maede Y: Serum Erythropoietin Level in Normal Dogs, Jpn. J. Vet. Sci. 52(4): 877-878, (1990).
• 37. Caıo J, Erslev AJ, Silver R, Miller O, Biıgegard G: Erythropoietin Production in Respons to Anemia or Hypoxia in the Nevvbom Rat, Blood, 60(4): 984-988, (1982).
• 38. Lange RD: In “Methods in investigative and diagnostic endocrinology, paıt III. Non-pituitary hormones” p. 1124 (S.A. Beıson and R.S. Yalow, ed.). North Holland Pub. Co., Amsterdam, (1973).
• 39. Fisher JW, Stuckey WJ, Lindholm DD, Abshire S: Extrarenal erythropoietin production. Isr. J. Med. Sci. 7: 991-992, (1971).
• 40. Davis ME, Bemdt WO: Renal methods for toxicology. In: principales and methods of toxicolog, 3rd ed., Ed. By Hayes WA., Raven press, Ltd., New York, (1994).