Sığırlarda lökosit artışı ile seyreden hastalıklarda, lökosit artışı ile lipit peroksidasyonu arasındaki ilişkinin araştırılması
Year 2007,
Volume: 10 Issue: 1-2, 13 - 16, 15.06.2007
Mehtap Çelîk
Burhanettin Baydaş
Abstract
Bu çalışma, lökosit artışının saptandığı hasta hayvanlarda, lökosit artışı ile lipit peroksidasyonu arasında ne tür bir ilişki olduğunu araştırmak üzere planlandı. Lökosit artışı saptanan 20 hasta ve 20 sağlıklı olmak üzere toplam 40 hayvan çalışmanın deney hayvan materyalini oluşturdu. Bu hayvanlardan alınan kan örneklerinden malondialdehit MDA ve indirgenmiş glutatyon GSH analizleri hemen yapıldı. Malondialdehit değerleri hasta hayvanlarda sağlıklı hayvanlara oranla oldukça yüksek bulunurken, aradaki farkın istatistiksel anlamda önemli olduğu saptandı P
References
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- Badwey JA, Kamowsky ML: Active oksigen species and the functions of phagocytic leukocytes. Ann Rev Biochem, 49: 695- 726, (1980).
- MacNee W, Rahman I: Is oxidative stress Central to the pathogenesis of chronic obstructive pulmonary disease? TRENDS in Molecular Medicine, 7: 55-62, (2001)
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- Yano E: Mineral fıber-induced malondialdehyde formation and effects of oxidant scavengers in phagocytic cells, Int. Arch. Occup. Environ. Health, 61: 19-23, (1988).
- Floyd RA: Basic free radical chemistry, Free Radicals In Aging. Edited By B.P.Yu Boca Raton, F.L: Crc P. 39-55, (1993).
- Fridovich I: The biology of oxygen radicals. Science Wash, 201: 875-880,(1978).
- Akkuş İ: Serbest Radikaller ve Fizyopatolojik Etkileri, Mimoza Yayınları, Konya, 1-60, (1995).
- Nayler VG: Basic mechanism involved in the protection of the ischaemic myocardium. The role'of calcium antagonsts, Drugs, 42:21-2,(1991).
- Fletcher RH and Fletcher $W: Glutathione and aging: Ideas and evidence. The Lancet, 8934: 1379-1380,(1994).
- Guteeridge JM: Lipid peroksidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41: 1819-1828, (1995).
- Esterbauer H, Schaur RJ, Zolner J: Chemistry and biochemistry of malondialdehide and related aldehydes. Free Rad Biol Med, 11: 82-128,(1991).
- Florence TM: The role of free radicals in disease. Aust N Z J Ophthalmol, 23: 3-7, (1995).
- Meistr A, Anderson ME: Glutathione. Ann. Rev. Biochem., 52: 711-760,(1983).
- Moustafa S A: Effect of glutathione (GSH) depletion on the serum levels of triiodothyronine (T3), thyroxine (T4), and T3/T4 ratio in aliyi alcohol-treated male rats and possible protection with zinc. Int J Toxicol, 20: 15-20, (2001).
- Ortolani O, Conti A, Raffaele de Gaudio A, Moraldi E, Cantini Q, Gianpaolo N: The effect of glutathione an N-Acetylcysteine on lipoperoxidative damage in patients with Early Septic Shock. Am. JRespir Crit Çare Med, 161: 1907-1911,(2000)
- Konuk T: Pratik Fizyoloji I, 2. baskı, A. Ü. Basımevi, Ankara, s: 71-73, (1981)
- Sushil JK, Mcvie R, Duett J, Herbst JJ: Erythrocyte mambrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes, 38: 1539 - 1543, (1989).
- Beutler E, Dubon O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med, 61: 882- 888,(1963).
- Cross CE, Halliwell B, Borish ET: Oxygen radicals and human disease, Davis Conference. Ann Intern Med, 107: 526-545, (1987).
- Boveris A: Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria. Packer, L. (Derleyen) Method in Enzymology, 105: 429-435, (1984).
- Witz G, Lawrie NJ, Amoruso MA, Goldstein BD: Inhibition by reactive aldehydes of superoxide anion radical production instimulated human neutrophils. Chem Biol Interact, 53: 13-23, (1985).
- Panus PC, Eddy LJ, Longenecker GL.J: Measurement of malonyldialdehyde production during sodium arachidonate- induced polymorphonuclear leukocyte aggregation. Pharmacol Methods, 13: 179-86,(1985).
- Hu S, Zheng L, Chen B, Xie J, Yang C: The role of the leukocytes in pathogenesis of secondary brain injury. Hunan Yi Ke Da Xue Xue Bao, 24: 56-8, (1999).
- Zhang H, Lin Y, Ding C: The role of neutrophils and interleukin- 8 in pathogenesis of asthma. Zhonghua Jie He He HuXiZaZhi, 24:225.7,(2001).
- Cetinkale O, Konukoglu D, Şenel O, Kemerli GD, Yazar S: Modulating the functions of neutrophils and lipid peroxidation by FK506 in a rat model of thermal injury. Burns, 25: 105-12, (1999).
- Lantos J, Temes G, Gobolos L, Jaberansari MT, Roth E: Is peripheral blood a reliable indicator of acute oxidative stress follovving heart ischemia and reperfusion? Med Sci Monit, 7: 1166-1170, (2001).
- Şato M, Miyazaki T, Nagaya T, Murata Y, Ida N, Maeda K, Seo H: Antioxidants inhibit tumor necrosis factor-alpha mediated stimulation of interleukin-8, monocyte chemoattractant protein-1, and collagenase expression in cultured human synovial cells. J Rheumatol, 23: 432-438, (1996).
- Staal FJ, Ela SW, Roederer M, Anderson MT, Herzenberg LA: Glutathione defıciency and human immunodefıciency virüs infection. Lancet, 339: 909-912, (1992).
Investigation of the relationship between increased leukocyte levels and blood lipid peroxidation in the sick cattle with leukocytosis
Year 2007,
Volume: 10 Issue: 1-2, 13 - 16, 15.06.2007
Mehtap Çelîk
Burhanettin Baydaş
Abstract
This study was designed to investigate whether there was a relationship between increased leukocyte levels and blood lipid peroxidation in cows with leukocytosis. Elevation or/and activation of leukocytes cause an increase in the production of free radicals leading to the lipid peroxidation. Malondialdehide MDA is the most important marker for lipid peroxidation. Forty cows were investigated. Twenty animals diagnosed with leukocytosis were chosen to represent sick group. Twenty healthy animals were chosen as Controls. Blood samples were drown to measure MDA and glutathione levels. It was found that MDA level was signifıcantly higher P
References
- Villa P, Saccani A, Sica A, Ghezzi P: Glutathine protects mice from lethal sepsis by limiting imflamation and potentiating hoşt defense. JlnfectDis., 15: 1115-20, (2002).
- Badwey JA, Kamowsky ML: Active oksigen species and the functions of phagocytic leukocytes. Ann Rev Biochem, 49: 695- 726, (1980).
- MacNee W, Rahman I: Is oxidative stress Central to the pathogenesis of chronic obstructive pulmonary disease? TRENDS in Molecular Medicine, 7: 55-62, (2001)
- Nowak D, Zieba M, Zawiasa D, Rozniecki J, Krol M: Changes of serum concentration of lipid lipid peroxidation products in patients with pneumonia. Monaldi Arch Chest Dis, 51: 188- 93, (1996).
- Novozhenov VG, Viazitlkii PO, Ermakov EV, Kolomoets NM, Chekushin Tl: Pathogenesis of acute pneumonia. Ter Arkh., 61: 84-87, (1989).
- Yano E: Mineral fıber-induced malondialdehyde formation and effects of oxidant scavengers in phagocytic cells, Int. Arch. Occup. Environ. Health, 61: 19-23, (1988).
- Floyd RA: Basic free radical chemistry, Free Radicals In Aging. Edited By B.P.Yu Boca Raton, F.L: Crc P. 39-55, (1993).
- Fridovich I: The biology of oxygen radicals. Science Wash, 201: 875-880,(1978).
- Akkuş İ: Serbest Radikaller ve Fizyopatolojik Etkileri, Mimoza Yayınları, Konya, 1-60, (1995).
- Nayler VG: Basic mechanism involved in the protection of the ischaemic myocardium. The role'of calcium antagonsts, Drugs, 42:21-2,(1991).
- Fletcher RH and Fletcher $W: Glutathione and aging: Ideas and evidence. The Lancet, 8934: 1379-1380,(1994).
- Guteeridge JM: Lipid peroksidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41: 1819-1828, (1995).
- Esterbauer H, Schaur RJ, Zolner J: Chemistry and biochemistry of malondialdehide and related aldehydes. Free Rad Biol Med, 11: 82-128,(1991).
- Florence TM: The role of free radicals in disease. Aust N Z J Ophthalmol, 23: 3-7, (1995).
- Meistr A, Anderson ME: Glutathione. Ann. Rev. Biochem., 52: 711-760,(1983).
- Moustafa S A: Effect of glutathione (GSH) depletion on the serum levels of triiodothyronine (T3), thyroxine (T4), and T3/T4 ratio in aliyi alcohol-treated male rats and possible protection with zinc. Int J Toxicol, 20: 15-20, (2001).
- Ortolani O, Conti A, Raffaele de Gaudio A, Moraldi E, Cantini Q, Gianpaolo N: The effect of glutathione an N-Acetylcysteine on lipoperoxidative damage in patients with Early Septic Shock. Am. JRespir Crit Çare Med, 161: 1907-1911,(2000)
- Konuk T: Pratik Fizyoloji I, 2. baskı, A. Ü. Basımevi, Ankara, s: 71-73, (1981)
- Sushil JK, Mcvie R, Duett J, Herbst JJ: Erythrocyte mambrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes, 38: 1539 - 1543, (1989).
- Beutler E, Dubon O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med, 61: 882- 888,(1963).
- Cross CE, Halliwell B, Borish ET: Oxygen radicals and human disease, Davis Conference. Ann Intern Med, 107: 526-545, (1987).
- Boveris A: Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria. Packer, L. (Derleyen) Method in Enzymology, 105: 429-435, (1984).
- Witz G, Lawrie NJ, Amoruso MA, Goldstein BD: Inhibition by reactive aldehydes of superoxide anion radical production instimulated human neutrophils. Chem Biol Interact, 53: 13-23, (1985).
- Panus PC, Eddy LJ, Longenecker GL.J: Measurement of malonyldialdehyde production during sodium arachidonate- induced polymorphonuclear leukocyte aggregation. Pharmacol Methods, 13: 179-86,(1985).
- Hu S, Zheng L, Chen B, Xie J, Yang C: The role of the leukocytes in pathogenesis of secondary brain injury. Hunan Yi Ke Da Xue Xue Bao, 24: 56-8, (1999).
- Zhang H, Lin Y, Ding C: The role of neutrophils and interleukin- 8 in pathogenesis of asthma. Zhonghua Jie He He HuXiZaZhi, 24:225.7,(2001).
- Cetinkale O, Konukoglu D, Şenel O, Kemerli GD, Yazar S: Modulating the functions of neutrophils and lipid peroxidation by FK506 in a rat model of thermal injury. Burns, 25: 105-12, (1999).
- Lantos J, Temes G, Gobolos L, Jaberansari MT, Roth E: Is peripheral blood a reliable indicator of acute oxidative stress follovving heart ischemia and reperfusion? Med Sci Monit, 7: 1166-1170, (2001).
- Şato M, Miyazaki T, Nagaya T, Murata Y, Ida N, Maeda K, Seo H: Antioxidants inhibit tumor necrosis factor-alpha mediated stimulation of interleukin-8, monocyte chemoattractant protein-1, and collagenase expression in cultured human synovial cells. J Rheumatol, 23: 432-438, (1996).
- Staal FJ, Ela SW, Roederer M, Anderson MT, Herzenberg LA: Glutathione defıciency and human immunodefıciency virüs infection. Lancet, 339: 909-912, (1992).