A Comparative Study on Microbial Air Quality of Radiology and Patient Waiting Rooms of a Full-Equipped Hospital in Aydın Province

Year 2024, , 12 - 24, 30.04.2024

Bahadır Törün , Aykan Özgür , Hacı Halil Bıyık , Esin Poyrazoğlu

https://doi.org/10.53433/yyufbed.1329620

Abstract

Every year many patients die due to hospital-acquired infections. These infections also put the lives of healthcare workers at risk. Additionally, hospitals are one of the main reservoirs of antimicrobial resistance. In this study, the effect of X-rays on species diversity and functions and antimicrobial resistance were observed. Metagenome analysis was performed on air samples taken from the x-ray room (radiation exposure) and the waiting room (non-radiation area) to examine whether the radiation affected the species present in the air. The diversity of microorganisms was analysed based on phylum, genus, and species levels. Functional profiling and resistance screening were also performed. X-ray radiation was found to have a major effect at the phylum level. It was observed that Proteobacteria species almost dominated the microbiome in the x-ray room (99%) while Actinobacteria species dominated the microbiome in the waiting room (84%). No significant differences were observed between the two areas in functional profiling. A total of thirty-eight functions were observed, twenty-four of which were overlapping. Antimicrobial resistance was not as diverse as expected. Only beta-lactam, penicillin, tetracycline, and lincomycin resistance genes were present (2364 reads belonging to four different genes). According to the results, it was observed that X-rays affected the air microbiome, as expected. A decrease in the number of microorganisms was expected, but it was also observed that the dominant microorganism types changed. On the other hand, no significant difference was found in terms of functional profiling and no significant antimicrobial resistance was observed.

Keywords

Air microbiome, Air quality, Metagenome, X-Ray room

Thanks

This study was conducted at Aydın Adnan Menderes University, Faculty of Science and Literature, Microbiology Laboratory.

Aydın İlindeki Tam Teşekküllü Bir Hastanenin Radyoloji ve Hasta Bekleme Odalarının Mikrobiyal Hava Kalitesinin Karşılaştırmalı Olarak İncelenmesi

Abstract

Her yıl çok sayıda hasta hastane kaynaklı enfeksiyonlar nedeniyle hayatını kaybetmektedir. Bu enfeksiyonlar sağlık çalışanlarının hayatını da riske atmaktadır. Ayrıca hastaneler, antimikrobiyal direncin ana rezervuarlarından biridir. Çalışmada X-ışınlarının tür çeşitliliği ve fonksiyonları üzerine etkisi ve antimikrobiyal direnç gözlemlenmiştir. Röntgen odasından (radyasyona maruz kalan) ve bekleme odasından (radyasyon olmayan alan) alınan hava örneklerine radyasyonun havada bulunan türleri etkileyip etkilemediğini görmek için metagenom analizi yapıldı. Mikroorganizmaların çeşitliliği şube, cins ve tür seviyelerinde analiz edildi. Ayrıca fonksiyonel profilleme ve direnç taraması yapıldı. X-ışını radyasyonunun şube düzeyinde büyük bir etkisi olduğu bulundu. Röntgen odasında Proteobacteria türleri mikrobiyomu neredeyse domine ettiği (%99), bekleme odasında Actinobacteria mikrobiyomu domine ettiği görüldü (%84). Fonksiyonel profillemede iki alan arasında önemli bir fark görülmedi. Yirmi dördü örtüşen toplam otuz sekiz fonksiyon gözlemlendi. Antimikrobiyal direnç beklenilen kadar çeşitli değildi. Sadece beta-laktam, penisilin, tetrasiklin ve lincomycin direnç genleri mevcuttu (dört farklı gene ait 2364 okuma). Çıkan sonuçlara göre, beklenti doğrultusunda, X-ışınlarının hava mikrobiyomunu etkilediği gözlemlendi. Mikroorganizma sayısında azalma bekleniyordu fakat baskın mikroorganizma türlerinin değiştiği de gözlemlendi. Fonksiyonel profilleme açısından önemli bir fark bulunamamış ve önemli bir antimikrobiyal direnç görülmemiştir.

Keywords

Hava kalitesi, Hava mikrobiyomu, Metagenom, Röntgen odası

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