The Mycobiome of The Gut of Willow Wood Borer, Xiphydria Prolongata (Hymenoptera: Xiphydriidae): A Rich Source of Rare Yeasts

Year 2024, , 45 - 52, 30.04.2024

Özgül Doğan , Ertan Mahir Korkmaz , Mahir Budak

https://doi.org/10.53433/yyufbed.1373533

Abstract

A high-throughput amplicon sequencing as a culture-independent approach was used to identify the gut mycobiome of the willow wood borer Xiphydria prolongata. The findings of this study are significant in terms of the insect-fungal interactions and indicate the unexpected richness of the mycobiome and the presence of many rare yeast species in the wood borer gut. A total of 40 fungal genera were found, and among them, only one endophytic fungus, Daldinia (Hypoxylaceae), has been previously reported in Xiphydria. Zygosaccharomyces siamensis is the most prevalent ascomycete species, while Rhodosporidiobolus colostri is the most abundant basidiomycetous yeast in X. prolongata. Some of the species identified here were known as very rare fungi such as Skoua fertilis, Chaetomium nepalense, R. colostri, and Vustinia terrae. This study is also the first report on S. fertilis and V. terrae in the insect gut flora. These funguses most likely aid in the digestion of lignocellulose in the gut of wood borer. Therefore, further research is required to know the source of acquisition and functional role of these yeast and their industrial potential.

Keywords

Endosymbiosis, Insect-fungal interactions, ITS1, Metabarcoding, Willow wood borer, Yeasts

Project Number

F-614

Odun Arısı Xiphydria Prolongata'nın (Hymenoptera: Xiphydriidae) Bağırsak Mikobiyomu: Nadir Mayalar İçin Zengin Bir Kaynak

Abstract

Söğüt odun arısı Xiphydria prolongata'nın bağırsak mikobiyomunu tanımlamak için kültürden bağımsız bir yaklaşım olarak yüksek verimli amplikon dizileme kullanılmıştır. Bu çalışmanın bulguları böcek-fungal etkileşimleri açısından önem taşımakta ve mikobiyomun beklenmedik zenginliğini ve ağaç kurdu bağırsağında birçok nadir maya türünün varlığını göstermektedir. Toplam 40 mantar cinsi bulunmuştur ve bunlar arasında sadece biri endofitik mantar olan Daldinia (Hypoxylaceae) daha önce Xiphydria'da rapor edilmiştir. Zygosaccharomyces siamensis en yaygın askomiset türü iken, Rhodosporidiobolus colostri X. prolongata'da en bol bulunan bazidiomiset mayadır. Tanımlanan türlerden Skoua fertilis, Chaetomium nepalense, R. colostri ve Vustinia terrae çok nadir bulunan mantarlar olarak bilinmektedir. Bu çalışma aynı zamanda S. fertilis ve V. terrae'nın böcek bağırsak florasındaki ilk raporudur. Bu funguslar büyük olasılıkla odun kurdunun bağırsağındaki lignoselülozun sindirimine yardımcı olmaktadır. Bu nedenle, bu mayaların elde edilme kaynağı ve işlevsel rolü ile endüstriyel potansiyellerinin bilinmesi için daha fazla araştırma yapılması gerekmektedir.

Keywords

Böcek-mantar etkileşimleri, Endosimbiyoz, ITS1, Mayalar, Metabarkodlama, Odun arıları

Ethical Statement

The author declares no conflicts of interest.

Supporting Institution

Sivas Cumhuriyet University BAP (CÜBAP)

Project Number

F-614

Thanks

The members of Evolutionary Bioinformatics Research Groups (EBRG) from Sivas Cumhuriyet University are thanked for their contributions in laboratory work.

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