Histopathological and Antioxidant Effects of Bromelain on Kidney Tissue of Tyloxapol-Induced Hyperlipidemic Rats

Year 2024, , 413 - 422, 31.08.2024

Ayşe Nurseli Sulumer , Esra Palabıyık , Bahri Avcı , Handan Uğuz , Hakan Aşkın

https://doi.org/10.53433/yyufbed.1381717

Abstract

This study was designed to investigate the nephroprotective effect of Bromelain against oxidative stress induced by Tyloxopol-induced hyperlipidemia in rats. Rats (n=6) were divided into three groups. I: Control (C) Group: No treatment was given and only distilled water was given; II: Tyloxapol (TX) Group: Group receiving Tyloxapol (400 mg/kg, i.p); III: Tyloxapol + Bromelain (TX+BR) Group: Group receiving Tyloxapol (400 mg/kg, i.p.) and group receiving Bromelain (250 mg/kg, o.d.) for 18 days. As a result of Tyloxapol administration, oxidative stress parameters SOD and CAT levels decreased, while MDA levels increased. In addition, histopathologic variations were detected in kidney sections. On the other hand, lipid peroxidation decreased and most of the studied parameters improved with combined administration (TX+BR group). According to the results obtained, bromelain may be a potent and alternative agent with protective effect for further studies.

Keywords

Bromelain, Histopathology, Hyperlipidemia, Kidney, Oxidative stress, Tyloxapol

Ethical Statement

Ethics committee approval was received for this study from Atatürk University Medical Experimental Application and Research Center (ATADEM) (dated 15.11.2021 and E-55885869-900-2100312890).

Thanks

We would like to thank Atatürk University Faculty of Science Molecular Biology/Genetics Departments for their support of our experimental study.

Tyloxapol ile İndüklenmiş Hiperlipidemik Sıçanların Böbrek Dokusu Üzerinde Bromelainin Histopatolojik ve Antioksidan Etkileri

Abstract

Bu çalışma, ratlarda Tyloxopol ile indüklenen hiperlipidemi neticesinde oluşan oksidatif strese karşı Bromelain’in nefroprotektif etkisinin incelenmesi için tasarlandı. Ratlar (n=6) üç grup altında sınıflandırıldı. I: Kontrol (C) Grubu: Herhangi bir uygulama yapılmayan ve sadece distile su verilen grup; II: Tyloxapol (TX) Grup: Tylaxopol (400 mg/kg, i.p) alan grup; III: Tyloxapol + Bromelain (TX+BR) Grup: Tylaxopol (400 mg/kg, i.p) alan ve 18 gün Bromelain (250 mg/kg, o.d.) alan grup. Tyloxapol uygulaması neticesinde oksidatif stres parametrelerinden SOD ve CAT seviyelerinde azalma meydana gelirken MDA seviyelerinde artış görüldü. Buna ek olarak böbrek kesitlerinde histopatolojik varyasyonlar tespit edildi. Bunun aksine çalışmada, kombine uygulama ile (TX+BR grubu) lipid peroksidasyonu azaldı ve çalışılan parametrelerin çoğunda iyileşme görüldü. Elde edilen sonuçlara göre bromelain’in daha sonraki çalışmalar için koruyucu etkiye sahip güçlü ve alternatif bir ajan olabileceği düşünülmektedir.

Keywords

Böbrek, Bromelain, Hiperlipidemi, Histopatoloji, Oksidatif stres, Tyloxapol

Ethical Statement

Bu çalışma için Atatürk Üniversitesi Tıbbi Deneysel Uygulama ve Araştırma Merkezi'nden (ATADEM) (15.11.2021 tarih ve E-55885869-900-2100312890) etik kurul onayı alındı.

Thanks

Deneysel çalışmamıza desteklerinden dolayı Atatürk Üniversitesi Fen Fakültesi Moleküler Biyoloji/Genetik Bölümlerine teşekkür ederiz.

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