Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones

Emre Sandık; Bektaş Sönmez; Sukran Yalçın Ozdilek

doi:10.53433/yyufbed.1483061

EN TR

Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones

Abstract

Skeletochronology is a powerful tool that provides information on the age of sea turtles. Digital images of histologically prepared humerus bone sections are commonly used for age determination. The quality of digital images, which is critical for robust age estimation, varies depending on the type and histological technique associated with microtome. This study aimed to compare the quality of digital image sections of humerus bones prepared by rotary and cryostat microtomes. The humerus bones of 11 juvenile stranded (dead) green turtles (mean CCL 292.1 mm) on Samandağ beach during the 2021 nesting season were used for the study. Three readers scored the quality of histologic sections prepared with two different microtomes and associated protocols, as well as the readability of the lines of arrested growth (LAGs), which they saw from 0 to 5. The Wilcoxon-signed rank-paired test was used to ascertain the disparities between the quality scores of the two protocols. In addition, the compatibility of readers was evaluated by determining the proportion of specimens with at least two identical scores for each protocol. The scores obtained from the cryostat microtome protocol had a wider variation compared to the rotary microtome protocol. The rotary microtome protocol has higher median score values (4) than the cryostat microtome protocol (3) (p<0,001). The compatibility of three cryostat microtome readers was lower than that of the rotary microtome. Although the cryostat microtome is less time-consuming and less costly, the results of this study showed that the rotary microtome protocol was preferable to the cryostat microtome protocol. Future studies may test techniques like various staining protocols to gain valuable insights into skeletochronology.

Keywords

Age determination, Cryostat, Green turtle, Microtome, Samandağ

Yeşil Kaplumbağa Humerus Kemiklerinde İskeletkronolojisi Yönteminde Döner ve Kriyostat Mikrotomun Karşılaştırılması

Abstract

İskeletkronolojisi, deniz kaplumbağalarının yaşı hakkında bilgi sağlamak için kullanılan güçlü bir araçtır. Histolojik olarak hazırlanmış humerus kemiklerinin enine kesitlerinin dijital görüntüleri yaş tayini için yaygın olarak kullanılmaktadır. Sağlıklı yaş tahmini için kritik öneme sahip olan dijital görüntülerin kalitesi, mikrotomla ilişkili histoloji tekniğine ve türüne bağlı olarak değişir. Bu çalışmanın amacı, döner ve kriyostat mikrotomlarla hazırlanan humerus kemiklerinin kesitlerinin dijital görüntülerinin kalitesini karşılaştırmaktır. Çalışma için 2021 yuvalama sezonu Samandağ kumsalında karaya ölü olarak vurmuş 11 juvenil yeşil kaplumbağanın (ortalama CCL 292,1 mm) humerus kemikleri kullanıldı. Üç okuyucu, iki farklı mikrotom ve ilgili protokollerle yapılan histolojik kesitlerin kalitesini ve gördükleri LAG'lerin okunabilirliğini 0 ile 5 arasında puanladı. İki protokolün kalite puanları arasındaki farklılıkları belirlemek için Wilcoxon işaretli eşleştirilmiş sıralama testi kullanılmıştır. Buna ek olarak, okuyucuların uyumluluğu, her protokol için en az iki aynı puana sahip numunelerin oranı belirlenerek değerlendirilmiştir. Kriyostat miktotom protokolünden elde edilen skorlar döner mikrotom protokolüne kıyasla daha geniş bir varyasyona sahipti. Döner mikrotom protokolü kriyostat mikrotom protokolünden daha yüksek medyan skor değerlerine sahiptir (p<0,001). Üç kriyostat okuyucusunun uyumluluğu (0,73) döner mikrotomunkinden (0,91) daha düşüktür. Kriyostat mikrotom tekniği daha az zaman alıcı ve daha az maliyetli olmasına rağmen, bu çalışmanın sonuçları rotary mikrotom protokolünün kriyostat mikrotom protokolüne tercih edildiğini göstermiştir. Gelecekteki çalışmalar, iskelet kronolojisi hakkında değerli bilgiler edinmek için çeşitli boyama protokolleri gibi diğer teknikleri test edebilir.

Keywords

Kriyostat, Mikrotom, Samandağ, Yaş belirleme, Yeşil kaplumbağa

Supporting Institution

The Scientific and Technological Research Council of Turkey (TUBITAK)

Project Number

122Z247

Ethical Statement

The authors declare that they have no known competing interests or personal relationships that could have appeared to influence the work reported in this paper.

Thanks

This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) Grant numbers 122Z247. We would like to thank Op. Dr. Hasan Keser from Çanakkale Mehmet Akif Ersoy State Hospital, Dr. Ali Fuat Çiçek in Pathology Laboratory, and technicians Hilal Köksal Elagöz and Gönül Cömert Özkan, Op. Dr. Gökhan BAŞTÜRK from Çanakkale Provincial Health Director, 7th Regional Directorate of Nature Conservation and National Parks and Samandağ Environmental Protection and Tourism Association for their contributions.

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Details

Primary Language

English

Subjects

Zoology (Other)

Journal Section

Research Article

Authors

Emre Sandık
0009-0009-9180-0698
Türkiye

Bektaş Sönmez ^*
0000-0002-8190-409X
Türkiye

Sukran Yalçın Ozdilek
0000-0001-8264-7606
Türkiye

Publication Date

December 31, 2024

Submission Date

May 13, 2024

Acceptance Date

September 19, 2024

Published in Issue

Year 2024 Volume: 29 Number: 3

DOI

https://doi.org/10.53433/yyufbed.1483061

IZ

https://izlik.org/JA62ZH33WF

APA

Sandık, E., Sönmez, B., & Yalçın Ozdilek, S. (2024). Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 29(3), 823-829. https://doi.org/10.53433/yyufbed.1483061

AMA

1.Sandık E, Sönmez B, Yalçın Ozdilek S. Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones. YYU JINAS. 2024;29(3):823-829. doi:10.53433/yyufbed.1483061

Chicago

Sandık, Emre, Bektaş Sönmez, and Sukran Yalçın Ozdilek. 2024. “Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29 (3): 823-29. https://doi.org/10.53433/yyufbed.1483061.

EndNote

Sandık E, Sönmez B, Yalçın Ozdilek S (December 1, 2024) Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29 3 823–829.

IEEE

[1]E. Sandık, B. Sönmez, and S. Yalçın Ozdilek, “Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones”, YYU JINAS, vol. 29, no. 3, pp. 823–829, Dec. 2024, doi: 10.53433/yyufbed.1483061.

ISNAD

Sandık, Emre - Sönmez, Bektaş - Yalçın Ozdilek, Sukran. “Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29/3 (December 1, 2024): 823-829. https://doi.org/10.53433/yyufbed.1483061.

JAMA

1.Sandık E, Sönmez B, Yalçın Ozdilek S. Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones. YYU JINAS. 2024;29:823–829.

MLA

Sandık, Emre, et al. “Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 29, no. 3, Dec. 2024, pp. 823-9, doi:10.53433/yyufbed.1483061.

Vancouver

1.Emre Sandık, Bektaş Sönmez, Sukran Yalçın Ozdilek. Comparison of Rotary and Cryostat Microtomes in the Skeletochronology on Green Turtle Humerus Bones. YYU JINAS. 2024 Dec. 1;29(3):823-9. doi:10.53433/yyufbed.1483061

Cited By

Discrepancies in the number of lines of arrested growth (LAG) in the tissues of the humerus and phalanx of sea turtles

The Science of Nature

https://doi.org/10.1007/s00114-025-01963-7