Research Article
BibTex RIS Cite

Sarıkamış(KARS) Yöresinde Pinus sylvestris L. Orman Topraklarından İzole Edilen Ektraselular Lakkaz Üreten Bacillus megaterium S1 İzolatının Karakterizasyonu

Year 2018, Volume: 23 Issue: 1, 79 - 86, 27.04.2018

Abstract

Bu
araştırmanın materyalini; Türkiye’nin, Kuzeydoğu Anadolu bölgesinden, Sarıkamış
yöresine ait Pinus sylvestris ormanlarından
toplanan toprak örnekleri oluşturdu. Toprak örneklerinden izole edilen, Bacillus
S1 izolatının, lakkaz enzimi üretimi yönünden pozitif olduğu tespit edildi. İzolatların
lakkaz üretme yetenekleri  0.2mM ABTS and
0.1mM CuSO% içeren Tryptic Soy Agar kullanılarak test edildi. Bacillus S1 izolatının,
morfolojik, fizyolojik ve biyokimyasal özelliklerine ve 16S rDNA gen bölgesinin
diziliş analizine göre Bacillus megaterium türüne
ait olduğu belirlendi. 

References

  • Abari, A. H., Emtiazi, G., & Roghanian, R. (2012). Production of none germinate spore ghost from a novel marine Bacillus with thermostable laccase activity. African Journal of Microbiology Research, 6(2), 393-402. Allos, M. M., & Hussein, A. A. Optimum Conditions for Laccase Production by Local Isolate of Bacillus Cereus B5. Journal of Al-Nahrain University Vol.18 (2), June, 2015, pp.133-140 Arık, B., Körlü, A. E., & Duran, K. (2008). Lakkaz Enzimlerinin Tekstilde Kullanım Alanları. Tekstil Teknolojileri Elektronik Dergisi, 2(2). Arias, M. E., Arenas, M., Rodríguez, J., Soliveri, J., Ball, A. S., & Hernández, M. (2003). Kraft pulp biobleaching and mediated oxidation of a nonphenolic substrate by laccase from Streptomyces cyaneus CECT 3335. Applied and Environmental Microbiology, 69(4), 1953-1958. Bozoglu, C., Adiguzel, A., Nadaroglu, H., Yanmis, D., & Gulluce, M. (2013). Purification and characterization of laccase from newly isolated thermophilic Brevibacillus sp.(Z1) and its applications in removal of textile dyes. Research Journal of Biotechnology Vol, 8, 9. Chandra, R., & Chowdhary, P. (2015). Properties of bacterial laccases and their application in bioremediation of industrial wastes. Environmental Science: Processes & Impacts, 17(2), 326-342. Claus, H. (2003). Laccases and their occurrence in prokaryotes. Archives of microbiology, 179(3), 145-150. Claus, H. (2004). Laccases: structure, reactions, distribution. Micron, 35(1), 93-96. Demiralp, B., Büyük, İ., Aras, S., & CANSARAN-DUMAN, D. (2015). Lakkaz enziminin endüstriyel ve biyoteknoloji alanında kullanımı. Türk Hijyen ve Deneysel Biyoloji Dergisi. Demissie, A. G., & Kumar, A. Isolation of Novel Bacteria Isolate from Soil for Production of Extra-Cellular Laccase Enzyme. International Journal of Emerging Technology and Advanced Engineering Website: www.ijetae.com (ISSN 2250-2459, ISO 9001:2008 Certified Journal, Volume 4, Issue 11, November 2014) Dias, A. A., Matos, A. J., Fraga, I., Sampaio, A., & Bezerra, R. M. (2017). An Easy Method for Screening and Detection of Laccase Activity. The Open Biotechnology Journal, 11(1). Ghumro, P. B., Shafique, M., Ali, M. I., Javed, I., Ahmad, B., Jamal, A., ... & Hameed, A. (2011). Isolation and screening of protease producing thermophilic Bacillus strains from different soil types of Pakistan. African Journal of Microbiology Research, 5(31), 5534-5539. Givaudan, A., Effosse, A., Faure, D., Potier, P., Bouillant, M. L., & Bally, R. (1993). Polyphenol oxidase in Azospirillum lipoferum isolated from rice rhizosphere: evidence for laccase activity in non-motile strains of Azospirillum lipoferum. FEMS Microbiology Letters, 108(2), 205-210. Gomaa, O. M., & Momtaz, O. A. (2007). 16S rRNA characterization of a Bacillus isolate and its tolerance profile after subsequent subculturing. Arab J Biotech, 10, 107-116. Jing, D. (2010). Improving the simultaneous production of laccase and lignin peroxidase from Streptomyces lavendulae by medium optimization. Bioresource technology, 101(19), 7592-7597. Joshi, B., Kabariya, K., Nakrani, S., Khan, A., Parabia, F. M., Doshi, H. V., & Thakur, M. C. (2013). Biodegradation of turquoise blue dye by Bacillus megaterium isolated from industrial effluent. American Journal of Environmental Protection, 1(2), 41-46. Kaushik, G., & Thakur, I. S. (2014). Production of laccase and optimization of its production by Bacillus sp. using distillery spent wash as inducer. Bioremediation Journal, 18(1), 28-37. Kıran, Ö. E., Çömlekçioğlu, U., Dostbil, N., 2006. Bazı Mikrobiyal Enzimler Ve Endüstrideki Kullanım Alanları. Ksü. Fen ve Mühendislik Dergisi, 9(1): 12-19. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L., & Pace, N. R. (1985). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences, 82(20), 6955-6959. Lončar, N., Božić, N., Lopez-Santin, J., & Vujčić, Z. (2013). Bacillus amyloliquefaciens laccase–from soil bacteria to recombinant enzyme for wastewater decolorization. Bioresource technology, 147, 177-183. Lu, L., Zhao, M., Wang, T. N., Zhao, L. Y., Du, M. H., Li, T. L., & Li, D. B. (2012). Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04. Bioresource technology, 115, 35-40. Machczynski, M. C., Vijgenboom, E., Samyn, B., & Canters, G. W. (2004). Characterization of SLAC: a small laccase from Streptomyces coelicolor with unprecedented activity. Protein science, 13(9), 2388-2397. Mahmoud, M. G., Rifaat, H. M., El Sayed, O. H., El Beih, F. M., & Selim, M. S. (2014). Production, and Characterization of Neutral Laccase from Marine Streptomyces lydicus. International Journal of PharmTech Research, 6(4), 1383-1391. Mishra, S. K., & Srivastava, S. K. (2016). Production of Extracellular Laccase from Bacterial Strain Bacillus Subtilis MTCC 1039 using Different Parameter. Biosciences Biotechnology Research Asia, 13(3), 1645-1650. Molina-Guijarro, J. M., Pérez Torres, J., Muñoz-Dorado, J., Guillén Carretero, F., Moya Lobo, R., Hernández Cutuli, M., & Arias Fernández, M. E. (2009). Detoxification of azo dyes by a novel pH-versatile, salt-resistant laccase from Streptomyces ipomoea. Nikiforov, Y. E., & Howles, P. N. (2001). Polymerase chain reaction. In Morphology Methods (pp. 181-207). Humana Press. Niladevi, K. N., Jacob, N., & Prema, P. (2008). Evidence for a halotolerant-alkaline laccase in Streptomyces psammoticus: purification and characterization. Process Biochemistry, 43(6), 654-660. Praveen Kumar, G. N., & Bhat Sumangala, K. (2012). Decolorization of azo dye Red 3BN by bacteria. International Research Journal of Biological Sciences, 1(5), 46-52. Rajeswari, M., & Bhuvaneswari, V. (2016). Production of extracellular laccase from the newly isolated Bacillus sp. PK4. African Journal of Biotechnology, 15(34), 1813-1826. Shah, A. I., & Jobanputra, J. B. 2017. Production and Partial Purification of Laccase Produced by Bacillus species Isolated from Contaminated Soil. International Journal of Innovative Research in Science, Engineering and Technology. 6: 17862-17869. Shah, M. P. (2014). Bioremedial application of Bacillus megaterium PMS82 in microbial degradation of acid orange dye. International Journal of Environmental Bioremediation & Biodegradation, 2(3), 93-99. Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme research. Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme research, 2011. Shukur, Y. N. (2015). Determination of Optimal Conditions for the Production of Laccase Enzyme by Local Isolate of Bacillus sp. Iraqi Journal of Science, 56(1A), 132-139. Singh, D., Narang, E., Chutani, P., Kumar, A., Sharma, K. K., Dhar, M., & Virdi, J. S. (2014). Isolation, Characterization and Production of Bacterial Laccase from Bacillus sp. In Microbial Diversity and Biotechnology in Food Security (pp. 439-450). Springer India. Singh, G., Bhalla, A., Kaur, P., Capalash, N., & Sharma, P. (2011). Laccase from prokaryotes: a new source for an old enzyme. Reviews in Environmental Science and Bio/technology, 10(4), 309-326. Siroosi, M., Amoozegar, M. A., & Khajeh, K. (2016). Purification and characterization of an alkaline chloride-tolerant laccase from a halotolerant bacterium, Bacillus sp. strain WT. Journal of Molecular Catalysis B: Enzymatic, 134, 89-97. Soden, D. M., O’callaghan, J., & Dobson, A. D. W. (2002). Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology, 148(12), 4003-4014. Sondhi, S., Sharma, P., Saini, S., Puri, N., & Gupta, N. (2014). Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4. PloS one, 9(5), e96951. Suzuki, T., Endo, K., Ito, M., Tsujibo, H., Miyamoto, K., & Inamori, Y. (2003). A thermostable laccase from Streptomyces lavendulae REN-7: purification, characterization, nucleotide sequence, and expression. Bioscience, biotechnology, and biochemistry, 67(10), 2167-2175. Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., ... & Whitman, W. (Eds.). (2011). Bergey's Manual of Systematic Bacteriology: Volume 3: The Firmicutes (Vol. 3). Springer Science & Business Media. Wang, C. L., Zhao, M., Li, D. B., Cui, D. Z., Lu, L., & Wei, X. D. (2010). Isolation and characterization of a novel Bacillus subtilis WD23 exhibiting laccase activity from forest soil. African Journal of Biotechnology, 9(34). Widsten, P., & Kandelbauer, A. (2008). Laccase applications in the forest products industry: a review. Enzyme and Microbial Technology, 42(4), 293-307. William, S., Feil, H., & Copeland, A. (2012). Bacterial genomic DNA isolation using CTAB. Sigma, 50, 6876.
Year 2018, Volume: 23 Issue: 1, 79 - 86, 27.04.2018

Abstract

References

  • Abari, A. H., Emtiazi, G., & Roghanian, R. (2012). Production of none germinate spore ghost from a novel marine Bacillus with thermostable laccase activity. African Journal of Microbiology Research, 6(2), 393-402. Allos, M. M., & Hussein, A. A. Optimum Conditions for Laccase Production by Local Isolate of Bacillus Cereus B5. Journal of Al-Nahrain University Vol.18 (2), June, 2015, pp.133-140 Arık, B., Körlü, A. E., & Duran, K. (2008). Lakkaz Enzimlerinin Tekstilde Kullanım Alanları. Tekstil Teknolojileri Elektronik Dergisi, 2(2). Arias, M. E., Arenas, M., Rodríguez, J., Soliveri, J., Ball, A. S., & Hernández, M. (2003). Kraft pulp biobleaching and mediated oxidation of a nonphenolic substrate by laccase from Streptomyces cyaneus CECT 3335. Applied and Environmental Microbiology, 69(4), 1953-1958. Bozoglu, C., Adiguzel, A., Nadaroglu, H., Yanmis, D., & Gulluce, M. (2013). Purification and characterization of laccase from newly isolated thermophilic Brevibacillus sp.(Z1) and its applications in removal of textile dyes. Research Journal of Biotechnology Vol, 8, 9. Chandra, R., & Chowdhary, P. (2015). Properties of bacterial laccases and their application in bioremediation of industrial wastes. Environmental Science: Processes & Impacts, 17(2), 326-342. Claus, H. (2003). Laccases and their occurrence in prokaryotes. Archives of microbiology, 179(3), 145-150. Claus, H. (2004). Laccases: structure, reactions, distribution. Micron, 35(1), 93-96. Demiralp, B., Büyük, İ., Aras, S., & CANSARAN-DUMAN, D. (2015). Lakkaz enziminin endüstriyel ve biyoteknoloji alanında kullanımı. Türk Hijyen ve Deneysel Biyoloji Dergisi. Demissie, A. G., & Kumar, A. Isolation of Novel Bacteria Isolate from Soil for Production of Extra-Cellular Laccase Enzyme. International Journal of Emerging Technology and Advanced Engineering Website: www.ijetae.com (ISSN 2250-2459, ISO 9001:2008 Certified Journal, Volume 4, Issue 11, November 2014) Dias, A. A., Matos, A. J., Fraga, I., Sampaio, A., & Bezerra, R. M. (2017). An Easy Method for Screening and Detection of Laccase Activity. The Open Biotechnology Journal, 11(1). Ghumro, P. B., Shafique, M., Ali, M. I., Javed, I., Ahmad, B., Jamal, A., ... & Hameed, A. (2011). Isolation and screening of protease producing thermophilic Bacillus strains from different soil types of Pakistan. African Journal of Microbiology Research, 5(31), 5534-5539. Givaudan, A., Effosse, A., Faure, D., Potier, P., Bouillant, M. L., & Bally, R. (1993). Polyphenol oxidase in Azospirillum lipoferum isolated from rice rhizosphere: evidence for laccase activity in non-motile strains of Azospirillum lipoferum. FEMS Microbiology Letters, 108(2), 205-210. Gomaa, O. M., & Momtaz, O. A. (2007). 16S rRNA characterization of a Bacillus isolate and its tolerance profile after subsequent subculturing. Arab J Biotech, 10, 107-116. Jing, D. (2010). Improving the simultaneous production of laccase and lignin peroxidase from Streptomyces lavendulae by medium optimization. Bioresource technology, 101(19), 7592-7597. Joshi, B., Kabariya, K., Nakrani, S., Khan, A., Parabia, F. M., Doshi, H. V., & Thakur, M. C. (2013). Biodegradation of turquoise blue dye by Bacillus megaterium isolated from industrial effluent. American Journal of Environmental Protection, 1(2), 41-46. Kaushik, G., & Thakur, I. S. (2014). Production of laccase and optimization of its production by Bacillus sp. using distillery spent wash as inducer. Bioremediation Journal, 18(1), 28-37. Kıran, Ö. E., Çömlekçioğlu, U., Dostbil, N., 2006. Bazı Mikrobiyal Enzimler Ve Endüstrideki Kullanım Alanları. Ksü. Fen ve Mühendislik Dergisi, 9(1): 12-19. Lane, D. J., Pace, B., Olsen, G. J., Stahl, D. A., Sogin, M. L., & Pace, N. R. (1985). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences, 82(20), 6955-6959. Lončar, N., Božić, N., Lopez-Santin, J., & Vujčić, Z. (2013). Bacillus amyloliquefaciens laccase–from soil bacteria to recombinant enzyme for wastewater decolorization. Bioresource technology, 147, 177-183. Lu, L., Zhao, M., Wang, T. N., Zhao, L. Y., Du, M. H., Li, T. L., & Li, D. B. (2012). Characterization and dye decolorization ability of an alkaline resistant and organic solvents tolerant laccase from Bacillus licheniformis LS04. Bioresource technology, 115, 35-40. Machczynski, M. C., Vijgenboom, E., Samyn, B., & Canters, G. W. (2004). Characterization of SLAC: a small laccase from Streptomyces coelicolor with unprecedented activity. Protein science, 13(9), 2388-2397. Mahmoud, M. G., Rifaat, H. M., El Sayed, O. H., El Beih, F. M., & Selim, M. S. (2014). Production, and Characterization of Neutral Laccase from Marine Streptomyces lydicus. International Journal of PharmTech Research, 6(4), 1383-1391. Mishra, S. K., & Srivastava, S. K. (2016). Production of Extracellular Laccase from Bacterial Strain Bacillus Subtilis MTCC 1039 using Different Parameter. Biosciences Biotechnology Research Asia, 13(3), 1645-1650. Molina-Guijarro, J. M., Pérez Torres, J., Muñoz-Dorado, J., Guillén Carretero, F., Moya Lobo, R., Hernández Cutuli, M., & Arias Fernández, M. E. (2009). Detoxification of azo dyes by a novel pH-versatile, salt-resistant laccase from Streptomyces ipomoea. Nikiforov, Y. E., & Howles, P. N. (2001). Polymerase chain reaction. In Morphology Methods (pp. 181-207). Humana Press. Niladevi, K. N., Jacob, N., & Prema, P. (2008). Evidence for a halotolerant-alkaline laccase in Streptomyces psammoticus: purification and characterization. Process Biochemistry, 43(6), 654-660. Praveen Kumar, G. N., & Bhat Sumangala, K. (2012). Decolorization of azo dye Red 3BN by bacteria. International Research Journal of Biological Sciences, 1(5), 46-52. Rajeswari, M., & Bhuvaneswari, V. (2016). Production of extracellular laccase from the newly isolated Bacillus sp. PK4. African Journal of Biotechnology, 15(34), 1813-1826. Shah, A. I., & Jobanputra, J. B. 2017. Production and Partial Purification of Laccase Produced by Bacillus species Isolated from Contaminated Soil. International Journal of Innovative Research in Science, Engineering and Technology. 6: 17862-17869. Shah, M. P. (2014). Bioremedial application of Bacillus megaterium PMS82 in microbial degradation of acid orange dye. International Journal of Environmental Bioremediation & Biodegradation, 2(3), 93-99. Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme research. Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme research, 2011. Shukur, Y. N. (2015). Determination of Optimal Conditions for the Production of Laccase Enzyme by Local Isolate of Bacillus sp. Iraqi Journal of Science, 56(1A), 132-139. Singh, D., Narang, E., Chutani, P., Kumar, A., Sharma, K. K., Dhar, M., & Virdi, J. S. (2014). Isolation, Characterization and Production of Bacterial Laccase from Bacillus sp. In Microbial Diversity and Biotechnology in Food Security (pp. 439-450). Springer India. Singh, G., Bhalla, A., Kaur, P., Capalash, N., & Sharma, P. (2011). Laccase from prokaryotes: a new source for an old enzyme. Reviews in Environmental Science and Bio/technology, 10(4), 309-326. Siroosi, M., Amoozegar, M. A., & Khajeh, K. (2016). Purification and characterization of an alkaline chloride-tolerant laccase from a halotolerant bacterium, Bacillus sp. strain WT. Journal of Molecular Catalysis B: Enzymatic, 134, 89-97. Soden, D. M., O’callaghan, J., & Dobson, A. D. W. (2002). Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology, 148(12), 4003-4014. Sondhi, S., Sharma, P., Saini, S., Puri, N., & Gupta, N. (2014). Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4. PloS one, 9(5), e96951. Suzuki, T., Endo, K., Ito, M., Tsujibo, H., Miyamoto, K., & Inamori, Y. (2003). A thermostable laccase from Streptomyces lavendulae REN-7: purification, characterization, nucleotide sequence, and expression. Bioscience, biotechnology, and biochemistry, 67(10), 2167-2175. Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., ... & Whitman, W. (Eds.). (2011). Bergey's Manual of Systematic Bacteriology: Volume 3: The Firmicutes (Vol. 3). Springer Science & Business Media. Wang, C. L., Zhao, M., Li, D. B., Cui, D. Z., Lu, L., & Wei, X. D. (2010). Isolation and characterization of a novel Bacillus subtilis WD23 exhibiting laccase activity from forest soil. African Journal of Biotechnology, 9(34). Widsten, P., & Kandelbauer, A. (2008). Laccase applications in the forest products industry: a review. Enzyme and Microbial Technology, 42(4), 293-307. William, S., Feil, H., & Copeland, A. (2012). Bacterial genomic DNA isolation using CTAB. Sigma, 50, 6876.
There are 1 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Erdal Öğün

Kerem Özdemir

Necati Özok This is me

Metin Ertaş

Fazlı Öztürk

Publication Date April 27, 2018
Submission Date December 20, 2017
Published in Issue Year 2018 Volume: 23 Issue: 1

Cite

APA Öğün, E., Özdemir, K., Özok, N., Ertaş, M., et al. (2018). Sarıkamış(KARS) Yöresinde Pinus sylvestris L. Orman Topraklarından İzole Edilen Ektraselular Lakkaz Üreten Bacillus megaterium S1 İzolatının Karakterizasyonu. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23(1), 79-86.