Atrazin ve Metolachlor’ un Bazı Buğday (Triticum aestivum L.) Varyetelerinde Büyüme Parametreleri ve GST Aktivitesi Üzerine Etkileri

Year 2021, Volume: 26 Issue: 1, 11 - 18, 30.04.2021

Songül Çanakcı Gülengül , Fadime Karabulut

Abstract

Bu çalışmada, üç buğday varyetesine ait fidelerde, atrazin ve metolachlor herbisitlerinin farklı konsantrasyonlarının yarattığı toksik etkiye bağlı fizyolojik ve biyokimyasal değişiklikler araştırılmıştır. Bitki materyali olarak, Triticum aestivum L. türüne ait buğday Bayraktar, İkizce ve Tosunbey cv. tohumları kullanılmıştır. Her bir buğday varyetesi için 15 günlük fideler, aynı sayıda fideden oluşan 4 gruba ayrılmış ve adı geçen herbisitler her birine 4 farklı (0, 100, 300 ve 1000 µM) dozda uygulanmıştır. Fidelere yapılan tüm uygulamalar için hidroponik ortam tercih edilmiştir. Genel olarak, fidelerin büyüme parametrelerinde (kök-sürgün uzama büyümesinde, fide mg KA. g-1 TA) azalmaya sebep olmuştur. Yapraklarda; klorofil a+b miktarında atrazinde sadece bayraktar için artışa (100 μM) sebep olsa da, metolachlor her üç varyetede de artışa (100 μM ve 300 μM) sebep olmuştur. Bu konsantrasyonların dışında kalan tüm konsantrasyonlarda hep azalma olmuştur. Karotenoid miktarı ise her iki herbisit için, üç varyetede de azalmayla sonuçlanmıştır. Buğday yapraklarında GST aktivitesinde kontrole kıyasla; atrazin için bayraktar fidelerinde azalış, diğerlerinde ise artış tespit edilmiştir. Metolachlorda ise her üç varyetede artış olup en yüksek dozlarında da (1000 μM) azalış görülmüştür.

Keywords

Triticum aestivum L., Atrazin, Metolachlor, Oksidatif stres, GST, Oxidative Stress

Supporting Institution

FÜBAP

Project Number

FF.17.20

Thanks

Fübap birimine katkılarından dolayı teşekkür ederiz.

Effects of Atrazine and Metolachlor on Growth Parameters and GST Activity in Some Wheat (Triticum aestivum L.) Varieties

Abstract

In this study, physiological and biochemical changes due to toxic effects created by different concentrations of atrazine and metolachlor herbicides in seedlings of three wheat varieties were investigated. As plant material, wheat of the species Triticum aestivum L. Bayraktar, İkizce and Tosunbey cv. seeds were used. For each wheat variety, 15-day-old seedlings were divided into 4 groups consisting of the same number of seedlings, and the aforementioned herbicides were applied at 4 different (0, 100, 300 and 1000 µM) doses to each. Hydroponic environment was preferred for all applications on seedlings. In general, it caused a decrease in the growth parameters of the seedlings (root-shoot elongation growth, seedling mg KA. g-1 TA). On the leaves; Although chlorophyll a+b caused an increase in atrazine only for Bayraktar (100 μM), metolachlor caused an increase in all three varieties (100 μM and 300 μM). There was always a decrease in all concentrations outside of these concentrations. The amount of carotenoid were resulted in a decrease in all three varieties for both herbicides. GST activity in wheat leaves compared to the control; For atrazine, a decrease in Bayraktar seedlings and an increase in others were detected. Metolachlor, on the other hand, increased in all three varieties and decreased at the highest doses (1000 μM).

Keywords

Atrazine, GST, Metolachlor, Oxidative Stress, Triticum aestivum L., Wheat

Project Number

FF.17.20

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