Van Topraklarından İzole Edilen Colpoda (Ciliophora, Colpodea) Türleri Üzerinde Morfolojik ve Moleküler Filogenetik Araştırmalar

Year 2023, Volume: 28 Issue: 3, 830 - 842, 29.12.2023

Pelin Erişen , İsmail Yıldız

https://doi.org/10.53433/yyufbed.1285464

Abstract

Bu makalede Van topraklarından izole edilen Colpoda Ö. F. Müller 1773 cinsine ait dört siliyat siliyat türünü hem morfolojik hem de 18S rDNA gen dizisine dayalı filogenetik olarak inceledik. C. lucida, C. magna ve C. maupasi morfolojik olarak literatür verileri ile uyumludur. Morfolojik karakterleri bakımından daha önce tanımlanmış herhangi bir popülasyon ile eşleşmeyen bir popülasyon ise Colpoda sp. olarak sunuldu. C. lucida ve C. magna Türkiye siliyat faunasına ilk kez bu çalışma ile kaydedilmiştir. Filogenetik analizlerde (ML ve BI) Colpoda cinsi üyeleri dış grup olarak kullanılan türlerden tam destek ile ayrılmıştır. Bu çalışmada izole edilen C. lucida ve C. magna filogenetik ağaçlarda daha önce tanımlanmış türdeşleri ile birlikte konumlanırken C. maupasi popülasyonunun morfolojik olarak benzer olmamasına karşın C. eucaudata ile daha yakın konumlanmıştır. C. lucida türdeşlerinin bazal pozisyonunda onlardan genetik olarak farklılaşmış olarak yer almaktadır. Colpoda sp. ise diğer hemcinslerinden farklı bir kol üzerinde Colpoda sp. (JF747216) popülasyonu ile kardeş pozisyonda ve ondan genetik olarak uzak pozisyonda konumlanmıştır. Colpoda sp.’nin filogenetik pozisyonu ve genetik farklılığı onun yeni tür olmaya aday olduğuna işaret etmektedir.

Keywords

Colpoda, Filogeni, Morfoloji, Toprak siliyatları, Van

Morphological and Molecular Phylogenetic Investigations on Colpoda (Ciliophora,Colpodea) Species Isolated from Van Soils

Abstract

In the present paper, we have investigated four ciliate species belonging to the genus Colpoda O. F. Müller, 1773 isolated from Van soil and examined both morphologically and molecular phylogenetically based on the 18S rDNA gene sequence. C. lucida, C. maupasi and C. magna are compatible morphologically with literature data. One population, which did not match any previously defined species in terms of morphological characters, was presented as Colpoda sp. C. lucida and C. magna were recorded for the first time in the ciliate fauna of Turkey with this study. In the phylogenetic analyses (ML and BI), the members of the genus Colpoda separated from the species used as the outgroup with full support. In the phylogenetic trees, the C. lucida and C. magna species isolated in this study clustered together with the same species described previously, while the C. maupasi population was more closely located with C. eucaudata, although not morphologically similar. C. lucida was located in the basal position of the other populations of the same species and slightly genetically differentiated from them. The Colpoda sp. isolated in this study is located on a different arm from its congeners, in a sister position with the Colpoda sp. (JF747216) population and in a genetically distant position from it. It's phylogenetic position and genetical difference from other populations of the Colpoda sp. from other populations indicating a candidate to be a new species.

Keywords

Colpoda, Morpholohy, Phylogeny, Soil ciliates, Van

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