Investigation of the Effects of Piperlongumine and Doxorubicin Combined Treatment on Cell Death via PTEN in HeLa Cells

Year 2024, Volume: 29 Issue: 1, 1 - 11, 30.04.2024

Gonca Güzelel , Hande Akalan , Türker Bilgen , Duygu Yaşar Şirin

https://doi.org/10.53433/yyufbed.1258515

Abstract

Doxorubicin (Dox), which is used in treating many types of cancer including cervix cancer nevertheless, its effect alone is low especially in recurrent cases. Therefore, investigating of agents that can increase the impact of Dox continues. The aim of the present study is to answer the question: Can Piperlongumine (PL) a natural alkaloid cause an increase in the efficacy of Dox in the HeLa cell line? In this study, the effects of Dox and PL on cell viability by MTT and Acridine orange/propidium iodide staining, and expression levels of the PTEN (Phosphatase and tensin homolog 10) gene by Real-Time PCR and Western-Blot were evaluated in HeLa cells. It was determined that PL combined with Dox increased cell death and suppressed cell proliferation. The PTEN gene expression was decreased in all experimental groups, but the PTEN protein phosphorylation increased in cultures treated with PL and when Dox/PL was combined. The fact that PL application increases the activation of PTEN, which is a tumor suppressor. This indicates that it can be used to increase the effectiveness of Dox in the treatment.

Keywords

Doxorubicin, Piperlongumine, PTEN

Supporting Institution

Tekirdağ Namık Kemal University Scientific Research Projects Unit

Project Number

NKUBAP.01.YL.20.267

HeLa Hücrelerinde Piperlongumin ve Doksorubisin Kombine Tedavisinin PTEN Yoluyla Hücre Ölümü Üzerine Etkilerinin Araştırılması

Abstract

Doksorubisin (Dox) birçok kanser türünün yanı sıra serviks kanseri tedavisinde de kullanılmaktadır ancak özellikle tekrarlayan vakalarda tek başına etkisi düşüktür. Bu nedenle Dox etkisini artırabilecek ajanların araştırılması devam etmektedir. Bu çalışmada amaç: Doğal bir alkaloid olan Piperlongumine (PL), HeLa hücre hattında Dox etkinliğini artırabilir mi? sorusuna cevap vermektir. Çalışmamızda, HeLa hücrelerinde MTT ve Akridin oranj/propidyum iyodür boyama ile Dox ve PL'nin hücre canlılığı, Real-Time PCR ve Western-Blot ile PTEN (Fostataz and tensin homolog 10) gen/proteininin ekspresyonu üzerindeki etkileri değerlendirilmiştir. Dox ile kombine edilen PL'nin hücre ölümünü arttırmış ve proliferasyonunu baskılamıştır. Tüm deney gruplarında PTEN gen ekspresyonu azalmıştır ancak PL ve Dox/PL kombine uygulanan kültürlerde PTEN protein fosforilasyonu artmıştır. PL uygulamasının bir tümör baskılayıcı olan PTEN aktivasyonunu arttırması tedavide Dox etkinliğini arttırmak amacı ile kullanılabileceğini göstermektedir.

Keywords

Doksorubisin, Piperlongumin, PTEN

Project Number

NKUBAP.01.YL.20.267

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