Rumen Ciliate (Ciliophora, Trichostomatia) Fauna of Goats and Sheep in Antalya, and Phylogeny of Rumen Ciliates

Year 2024, Volume: 29 Issue: 2, 429 - 437, 31.08.2024

Gözde Gürelli , Nuray Yürücüoğlu

https://doi.org/10.53433/yyufbed.1458622

Abstract

In this study, the rumen ciliate fauna of goats (Capra aegagrus hircus) and sheep (Ovis aries) in Antalya was detected, and phylogenetic analyses were performed using 18S rDNA gene sequences of 68 rumen ciliates obtained from GenBank. The average ciliate density in the rumen of 6 goats and 11 sheep in Antalya was 124.0 × 104 cells mL–1 and 46.3 × 104 cells mL–1, respectively. Eight genera, 15 species, and 6 morphotypes have been identified in goats, and 8 genera, 19 species, and 7 morphotypes in sheep. Entodinium simulans was observed in all goats with a prevalence of 100%, while Metadinium affine and Entodinium bursa were identified in a single goat with a prevalence of 16.7%. Entodinium simulans in sheep had a prevalence of 81.8%, and in 10 of the 11 sheep examined, Enoploplastron triloricatum, Entodinium dilobum, E. longinucleatum, E. rectangulatum, E. simplex, and E. simulans m. lobosospinosum were detected in a single sheep with a prevalence of 9.1%. The rumen ciliate fauna of goats and sheep generally consists of Entodinium spp. (the average relative abundance for goats is 88.7%, and the average relative abundance for sheep is 52.2%). According to phylogenetic analyses, the Ophryoscolecinae, Entodiniinae, and Diplodininae subfamilies within the family Ophryoscolecidae have a monophyletic origin. The genera Isotricha, Dasytricha, Ophryoscolex, Epidinium, Entodinium, Diplodinium, and Ostracodinium are also monophyletic.

Keywords

Antalya, Goat, Phylogeny, Rumen, Sheep, Trichostomatia

Antalya’daki Keçi ve Koyunların İşkembe Siliyat (Ciliophora, Trichostomatia) Faunası ve İşkembe Siliyatlarının Filogenisi

Abstract

Bu çalışmada Antalya’daki keçi (Capra aegagrus hircus) ve koyunların (Ovis aries) işkembe siliyat faunası tespit edilmiş ve GenBank’tan elde edilen 68 işkembe siliyatına ait 18S rDNA gen dizileri kullanılarak filogenetik analizler yapılmıştır. Antalya’daki 6 keçi ve 11 koyunun işkembesindeki ortalama siliyat yoğunluğu sırasıyla, 124.0 × 104 hücre mL–1 ve 46.3 × 104 hücre mL–1’dir. Keçide 8 cins, 15 tür ve 6 morfotip, koyunda 8 cins, 19 tür ve 7 morfotip teşhis edilmiştir. Entodinium simulans %100 yaygınlıkla tüm keçilerde, Metadinium affine ve Entodinium bursa ise %16.7 yaygınlıkla tek bir keçiden gözlemlenmiştir. Koyunlarda Entodinium simulans %81.8 yaygınlıkla incelenen 11 koyunun 10’unda, Enoploplastron triloricatum, Entodinium dilobum, E. longinucleatum, E. rectangulatum, E. simplex, E. simulans m. lobosospinosum ise %9.1 yaygınlıkla tek bir koyundan tespit edilmiştir. Keçi ve koyunların işkembe siliyat faunası genelde Entodinium spp.’den meydana gelmektedir (keçiler için ortalama nispi bolluk %88.7, koyunlar için ortalama nispi bolluk %52.2). Filogenetik analizlere göre Ophryoscolecidae ailesi içinde Ophryoscolecinae, Entodiniinae ve Diplodininae alt-aileleri monofiletik orijine sahiptirler. Isotricha, Dasytricha, Ophryoscolex, Epidinium, Entodinium, Diplodinium ve Ostracodinium cinsleri de monofiletik kökenlidir.

Keywords

Antalya, Filogeni, İşkembe, Keçi, Koyun, Trichostomatia

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