Neonatal Buzağı İshallerinin İmmunokromotografik Test Kitleri İle Hızlı Etiyolojik Teşhisi

Year 2013, Volume: 24 Issue: 3, 123 - 128, 01.09.2013

Nuri Altuğ Nazmi Yüksek Cumali Özkan İhsan Keleş Yıldıray Başbuğan Zahid Tevf Ağaoğlu Abdullah Kaya Yakup Akgül

Abstract

In the present study, the most important etiologic agents of diarrhoea were diagnosed using an immunochromatografic method. In this study, 51 calves with diarrhoea were used as material. Fecal samples were obtained from the calves with diarrhoea using rectal stimuli and etiological diagnosis was made using a quick immunochromatografic test kit. Furthermore, parasitological examination of the feces was made then treatment was applied according to diagnosis. One or more than one enteropathogen was diagnosed in 64.7% calves. The most common culprit were E. coli K99 (27.45%) and rotavirus (27.45%) infection(s) as alone or mix. Coronavirus determined only in one calf (1.96%). Mix etiology (3.92% E.coli K99 + Rotavirus, 1.96% E.coli K99 + E.coli CSA 31A, 1.96% Rotavirus + Cryptosporidium) was determined at 7.84%. In diarrhoeic calves, 11.76% of them had only parasiter enteropathogens (Eimeria 5.88%, Cryptosporidium 3.92%, Ascarid 1.96%). As a result; quick immunochromatografic test kits can be used to determine enteropathogens causing calf diarrhoea. Prophylactic and administrative precautions have to be taken against determined enteropathogens.

Keywords

Calf, Diarrhoea, Enteropathogen, Rapid diagnostic test, Diagnosis

Neonatal Buzağı İshallerinin İmmunokromotografik Test Kitleri İle Hızlı Etiyolojik Teşhisi

Abstract

Bu çalışmada, ishalin en önemli etiyolojik etkenlerinin hızlı bir immunokromotografik metodla teşhisi amaçlandı. Çalışmada, 51 ishalli buzağı kullanıldı. İshalli buzağılardan rektal uyarımla steril dışkı kaplarına dışkı örnekleri alındı ve immunokromotografik hızlı diagnostik test kitleri ile enteropatojen teşhisi yapıldı. Ayrıca parazitolojik muayeneleri yapıldı ve etiyolojik teşhis sonuçlarına göre tedavi uygulandı. Buzağıların %64.7’sinde bir veya daha fazla enteropatojen tespit edildi. Tek veya miks olarak en çok E. coli K99 (%27.45) ve rotavirus (%27.45) saptandı. Coronavirus sadece bir buzağıda (%1.96) belirlendi. Buzağıların %7.84’inde miks etiyoloji (%3.92 E.coli K99 + Rotavirus, %1.96 E.coli K99 + E.coli CSA31A, %1.96 Rotavirus + Cryptosporidium) tespit edildi. İshalli buzağıların % 11.76’sında ise sadece paraziter enteropatojenler (Eimeria %5.88, Criptosporidium %3.92, Ascarid %1.96) belirlendi. Sonuç olarak; buzağı ishallerindeki en önemli etiyolojik faktörlerin belirlenmesinde hızlı immunokromotografik test kitlerinin rahatlıkla kullanılabileceği, belirlenen enteropatojenlere karşı gerekli proflaktik ve yönetimsel önlemlerin acilen alınması gerektiği kanısına varıldı.

Keywords

Buzağı, İshal, Enteropatojen, Hızlı diagnostik test, Teşhis

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