Deneysel Olarak Diyabet Oluşturulmuş Sıçanlarda Hba1c, Mda, Gsh-Px Ve Sod Miktarlarının Tayini

Year 2013, Volume: 24 Issue: 2, 51 - 54, 01.06.2013

Sevim ÇİFTÇİ Yegin Nihat Mert

Abstract

İnsülin sekresyonu veya etkisinde yetmezliği ile karakterize olan ve günümüzde çok yaygın olan Diabetes Mellitus üzerinde yapılan bu çalışmada 7-8 haftalık Wistar cinsi sıçanlar kullanıldı. Rastgele seçilen 10 sıçan kontrol, 20 sıçan diyabetli grup olmak üzere 2 grup oluşturuldu. Diyabetli gruba 45 mg/kg düzeyinde streptozotosin intraperitoneal uygulandı. Tüm hayvanlarda HbA1c, MDA, GSH-Px ve SOD düzeylerine bakıldı. HbA1c diyabetli grupta P<0.001 düzeyinde artış gösterirken, MDA düzeyinde saptanan yükselme istatiksel önem göstermemiştir (P>0.05). GSH-Px düzeyi diyabetli grupta azalmış (P<0.05), SOD miktarı ise diyabetli grupta artmıştır. Sonuç olarak, HbA1c’nin Diabetes Mellitus tanısında kullanılabilecek önemli bir parametre olduğuna ve Diabetes Mellitus’ta antioksidan enzim değişimlerinin hastalığın prognozu açısından önemli olabileceği, klinisyenlerin Diabetes Mellitus’ta bu parametrelere dikkat etmelerinin yerinde olacağı sonucuna varılmıştır.

Keywords

Diabetes Mellitus, Hemoglobin A1c, Glutatyon peroksidaz, Malon dialdehit, Rat, Süperoksit dismutaz

Investigation on the HbA1c, MDA, GSH-Px and SOD Levels in Experimentally Diabetic Rats

Abstract

Diabetes Mellitus is the results of deficiency of insulin secretion and effect was studied on Wistar rats in this research. Rats were randomly divided into 2 groups, 10 rats were healthy control and 20 rats were sick group. To develop experimental diabetes 45 mg/kg streptozotocine was administrated intraperiteonally to 20 rats in sick group. HbA1c, MDA, GSH-Px and SOD analysis were performed to blood samples of all rats. The levels HbA1c were increased significantly P<0.001 in sick group. Increased MDA levels in sick group didn’t show statistically significance (P>0.05). GSH-Px activity was significantly decreased in sick group, SOD activity was increased but statistical significance was not detected (P≤0.05). As conclusion, HbA1c was important parameters for the diagnosis at Diabetes mellitus, antioxidant enzyme activity changes could be important for the prognosis of Diabetes mellitus and clinicians must be careful and take in consideration of these changes during the treatment of their diabetic patient.

Keywords

Diabetes Mellitus, Glutathione Peroxidase, Hemoglobin A1c, Malonaldehyde, Rat, Superoxide Dismutase

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