Diyarbakır Bölgesindeki Sığırlarda Patojenik Leptospira spp Prevalansının Real-time PCR Yöntemi ile Tespiti

Year 2012, Volume: 23 Issue: 3, 137 - 139, 01.09.2012

Simten Yesılmen Neval Berrin Arserım Nurettin Isık Hasan Icen

Abstract

Keywords

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Diyarbakır Bölgesindeki Sığırlarda Patojenik Leptospira spp Prevalansının Real-time PCR Yöntemi ile Tespiti

Abstract

Bu çalışmada Diyarbakır’daki kesimhanelerde kesilen sığırların kan ve idrar örneklerinde real time PCR yöntemiyle patojenik Leptospira prevalansının ortaya konulması amaçlanmıştır. Bu çalışmanın materyalini üç büyük kesimhanedeki sığırlardan 96 sığırdan toplanan idrar ve kan örnekleri oluşturdu. Bu örneklerde patojen Leptospiraların varlığı real-time PCR yöntemiyle araştırıldı. Patojenik Leptospiraları etkenlerini varlığını ortaya koymak için hap 1 geninin tespit etme temeline dayanan yöntem kullanılmıştır. Leptospira şüpheli 96 sığırdan elde edilen idrar örneklerinden 9 (%9.4) adetinde hap1 geninin varlığı tespit edilmiştir. Aynı hayvanlardan alınan kan örneklerinde pozitifliğe rastlanılmamıştır. Bu nedenle, taşıyıcı hayvanların erken teşhisi, leptospiral enfeksiyonun diğer hayvanlara ve insanlara bulaşmasının önlenmesi bakımından son derece önemlidir

Keywords

Leptospira, Real-Time PCR, Hap 1, Sığır

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