Kovada, Eğirdir ve Karacaören II Baraj Göllerinde Yaşayan Sudak Balığı (Sander lucioperca L., 1857) Solungaçlarındaki Glikokonjugatların Histokimyasal Yapısı

Year 2012, Volume: 23 Issue: 1, 29 - 33, 01.03.2012

Emel Demirbağ  seval Kelek  kenan Çınar

Abstract

Bu çalışmada Kovada, Eğirdir ve Karacaören II Baraj Göllerinde yaşayan sudak balığı (Sander lucioperca) solungaçlarındaki glikokonjugat karakterinin belirlenmesi amacıyla 15 erişkin sudak balığına ait solungaç örnekleri kullanıldı. Kovada ve Eğirdir Gölleri’nde yaşayan sudakların solungaç mukus hücrelerinde çok güçlü WGA (Triticum vulgaris aglutinin) ve orta yoğunlukta SBA (Glycine max aglutinin) reaksiyonu tespit edildi. Karacaören II Baraj Gölü’nde yaşayan sudakların solungaçlarında UEA-I (Ulex europaeus aglutinin-I) reaksiyonuna rastlanmadı. Her üç gölde yaşayan sudakların solungaçlarındaki klorid hücrelerde Con A (Canavalia ensiformis aglutinin) ve WGA pozitif glikokonjugat bulunmadığı belirlendi

Keywords

Sudak, Sander lucioperca, Solungaç, Histokimya, Lektin

The histochemical structure of glycoconjugates in gills of zander (Sander lucioperca L., 1857) living in Kovada, Eğirdir and Karacaören II Dam Lakes

Abstract

In this study, the gill samples of 15 adult zander were used to determine the character of glycoconjugat in gills of zander (Sander lucioperca) living in Kovada, Eğirdir and Karacaören II Dam Lakes. The very strong WGA (Triticum vulgaris agglutinin) and moderate SBA (Glycine max agglutinin) reactions were detected in mucous cells of gills of zander living in Kovada and Eğirdir lakes. There was no reaction against to UEA-I (Ulex europaeus agglutinin-I) in gills of zander living in Karacaören II Dam Lake. It was identified that there were not Con A (Canavalia ensiformis agglutinin) and WGA positive glycoconjugates in chloride cells of gills of zander living in all three lakes. 

Keywords

Zander, Sander lucioperca, Gill, Histochemistry, Lectin

References

• Arellano JM, Storch V, Sarasquete C (2004). Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. J Appl Ichthyol, 20, 452–460.
• Bordas MA, Balebona MC, Chabrillon M, Rodriquez-Maroto JM, Morinigo MA (2003). Influence of temperature and salinity on the adhesion to mucous surfaces of gilt-head seabream (Sparus auratus L.) of pathogenic strains of Vibrio alginolyticus and Listonella anguillarum. Bull Eur Ass Fısh Pathol, 23, 273–280.
• Burkhardt-Holm P (1997). Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochem J, 29, 893-899.
• Calabro C, Albanese MP, Lauriano ER, Martella S, Licata A (2005). Morphological, histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus. Folia Histochem Cytobiol, 43 (1), 51–56.
• Diaz AO, Garcia AM, Goldemberg AL (2005). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). Sci Mar, 69(4), 545-553.
• Diaz AO, Garcia AM, Escalante AH, Goldemberg AL (2010). Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae). J Fish Biol, 77, 1665-1673.
• Dunel EB, Sebest P, Chevalier C, Simon B, Bart HL (1996). Morphological changes indiced by acclimation high pressure in the gill epithelium of the freshwater Yellow Eel. J Fish Biol, 48, 1018–1022.
• Genten F, Terwinghe E, Danguy A (2009). Atlas of fish histology. 215, Enfield, New Hampshire, USA.
• Hilary M, Lease-Hansen-James A, Bergman-Harold L, Meyer-Joseph S (2003). Structural changes in gills of Lost River suckers exposed to elevated pH and ammonia concentrations. Comp Biochem Physiol Part C: Toxicol Pharmacol, 134, 491–500.
• Hughes GM, Morgan M (2008). The structure of fish gills in relation to their respiratory function. Biol Rev, 48 (3), 419–475.
• Jung KS, Ahn MJ, Lee YD, Go GM, Shin TK (2002). Histochemistry of six lectins in the tissues of the flat fish Paralichthys olivaceus. J Vet Sci, 3(4), 293-301.
• Laurent P, Perry SF (1990). Effects of cortisol on gill chloride cell morphology and ionic uptake in the freshwater trout, Salmo gairdneri. Cell Tissue Res, 259, 429-442.
• Ledy K, Giamberini L, Pihan PC (2003). Mucous cell responces in gill and skin of Brown trout Salmo trutta fario in acidic, aluminium containing stream water. Dis Aquat Organ, 56, 235-240.
• Manera M, Simoni E, Dezfuli BS (2001). The effect of dexamethasone on the occurrence and ultrastructure of rodlet cells in goldfish. J Fish Biol, 59, 1239-1248.
• Mattey DL, Morgan M, Wright DE (2006). Distribution and development of rodlet cells in the gills and pseudobranch of the bass, Dicentrachus labrax (L). J Fish Biol, 15 (3), 363–370.
• Sarasquete C, Gisbert E, Ribeiro L, Vieira L, Dinis MT (2001). Glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development. Eur J Histochem, 45, 267-278.
• Timur G (2008). Balık anatomisi,1. basım, 111, Nobel yayınevi, Ankara.