Östrüs,Anöstrüs ve Yalancı Gebelikteki Kedilerin Dişi Üreme Organlarında Plazma Hücreleri ve Alpha-Naphthyl Acetate Esterase Pozitif Lenfositlerin Dağılımı

Year 2011, Volume: 22 Issue: 3, 169 - 173, 01.09.2011

Turan Karaca Sevket Arıkan Hakan Kalender Ilknur PIR Yagcı Mecit Yoruk

Abstract

Keywords

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Östrüs,Anöstrüs ve Yalancı Gebelikteki Kedilerin Dişi Üreme Organlarında Plazma Hücreleri ve Alpha-Naphthyl Acetate Esterase Pozitif Lenfositlerin Dağılımı

Abstract

Kedilerde anöstrüs, östrüs ve yalancı gebelik dönemlerinde dişi genital kanal organları ve ovaryumlarında, plasma hücresi ve alpha naphthyl acetate esterase (ANAE) positif lenfositlerin dağılımı, histokimyasal teknikler kullanılarak ışık mikroskopik olarak belirlendi. Plasma hücrelerinin incelenmesi için doku örnekleri formol-alkol solüsyonunda 48 saat tespit edilip parafinde bloklandı. Altı mikron kalınlığındaki kesitler metil green - pironin ile boyandı. ANAE için doku örnekleri formolsükroz solüsyonunda (pH 6.8) +4C ‘de 22 saat, sonra Holt solüsyonunda yine aynı şartlarda 22 saat fikse edildi. ANAE boyaması için 8 μm kalınlığındaki kriyostat kesitleri pH 6.2’de inkübe edildi. Kedilerde anöstrüs döneminde plasma hücre sayısı korpus uteride kornu uteriye göre anlamlı derecede azdı (P

Keywords

Kelimeler: Plasma hücresi, Lenfosit, Östrüs siklusu, Yalancı gebelik, Kedi

References

• Arikan Ş, Yigit AA, Kalender H (2009). Size distribution of luteal cells during pseudopregnancy in domestic cats. Reprod Dom Anim, 44, 842– 845.
• Aştı RN, Tanyolaç A, Kurtdede N, Özen A (1997). Distribution of T and B Lymphocytes in Angora Goat Lymphoid Tissues. Tr J Vet Anim Sci (In Turkish), 21, 99–105.
• Bischof RJ, Brandon MR, Lee CS, (1995). Cellular immune responses in the pig uterus during pregnancy. J Reprod Immunol, 29, 161–178.
• Brannstrom M, Giesecke L, Moore IC, Van Den Heuvel CJ, Robertson SA (1994). Leukocyte subpopulations in the rat corpus luteum during pregnancy and pseudopregnancy. Biol Reprod, 50, 1161-1167.
• Butterworth JC, English RV, Jordan HL, Tompkins MB (2001). Distribution of immune cells in the female reproductive tract in uninfected and FIV infected cats. Vet Immunol Immunopathol, 83, 37- 51.
• Cemek M, Enginar H, Karaca T, Unak P (2006). In vivo radioprotective effects of nigella sativa L oil and reduced glutathione against irradiation-ınduced oxidative injury and number of peripheral blood lymphocytes in rats. Photochem Photobiol, 82, 1691–1696.
• Croy BA, Wood W, King GJ (1987). Evaluation of intrauterine immune suppression during pregnancy in a species with epitheliochorial placentation. J Immunol, 139, 1088-1095.
• Dantzer V (1985). Electron microscopy of the initial stages of placentation in the pig. Anat Embryol, 172, 281-293.
• Enginar H, Cemek M, Karaca T, Unak P (2007). Effect of grape seed extract on lipid peroxidation, antioxidant activity and peripheral blood lymphocytes in rats exposed to x-radiation. Phytother Res, 21, 1029– 1035.
• Hunt J (1994). Immunologically relevant cells in the uterus. Biol Reprod, 50, 461-466.
• Kaeoket K, Persson E, Dalın AM (2003). Influence of pre-ovulatory insemination and early pregnancy on the infiltration by cells of the immune system in the sow endometrium. Anim Reprod Sci, 75, 55–71.
• Karaca T, Yörük M, Uslu S, Uslu BA, Çetin Y (2009). Distribution of plasma cells and alpha-naphthyl acetate esterase (ANAE) positive lymphocytes in the reproductive tract of female goats in the preimplantation phase. YYU Vet Fak Derg, 20, 1 – 5.
• Karaca T, Arikan Ş, Kalender H, Yörük M (2007). Distribution and quantitative patterns of T lymphocytes in the female reproductive tract and ovary throughout the oestrus cycle of Angora Goats. Medycna Wet, 63, 1320-1323.
• King GJ (1988). Reduction in uterine intra-epithelial lymphocytes during early gestation in pigs. J Reprod Immunol, 14, 41-46.
• Maiti NK, Saini SS, Sharma SN (1990). Histochemical studies on chicken peripheral blood lymphocytes. Vet Res Commun, 14, 207-210.
• Martinez CM, Buendia AJ, Sanchez J, Navarro JA (2005). Immunophenotypical characterization of lymphocyte subpopulations of the uterus of non-pregnant and pregnant goats. Anat Histol Embryol, 34, 240–246.
• Paape SR, Shille VM, Seto H, Stabenfeldt GU (1975). Lutealactivity in the pseudopregnant cat. Biol Reprod, 13, 470–474.
• Parr MB, Parr EL (1994). Mucosal immunity in the female and male reproductive tracts. In: Handbook of Mucosal Immunology Eds. Ogra PL, Mestecky J, Lamm ME, Strober W, Mc Ghee JR, Bienestock J, Academic Press, New York.
• Perez-Martinez M, Luna J, Mena R, Romano MC (2002). Lymphocytes and T lymphocyte subsets are regionally distributed in the female goat reproductive tract: influence of the stage of the oestrus cycle. Res Vet Sci, 72, 115–121.
• Roitt I, Male D, Grossi C (1998). An introduction to the immune system and cells involved in the immune response. In: Immunology, 5th ed., Roitt I, Brostoff J, Male D (Eds.), Mosby international Ltd, London.
• Salamonsen LA (1999). Role of proteases in implantation. Rev Reprod, 4, 11–22.
• Sharkey A (1998). Cytokines and implantation. Rev Reprod, 3, 52–61.
• Tabibzadeh S (1991). Human endometrium: an active site of cytokine production and action. Endocr Rev, 12, 272-290.
• Tekin Ş, Hansen PJ (2004). Regulation of numbers of macrophages in the endometrium of the sheep by systemic effects of pregnancy, local presence of the conceptus, and progesterone. Am J Reprod Immunol, 51, 56–62.
• Tizard IR (1996). An introduction: The trapping of foreign material, the second phagocytic cell population (macrophages) and B cells and their response to antigen. In: Veterinary Immunology, 5th ed., Tizard IR, (Ed.), W.B. Saunders Co., Pennsylvania.
• Tsutsui T, Stabenfeldt GH (1993). Biology of ovarian cycles, pregnancy and pseudopregnancy in the domestic cat. J Reprod Fertil Suppl, 47, 29– 35.
• Wira CR, Richardson J, Prabhala R (1994). Endocrine regulation of mucosal immunity: Effect of sex-hormones and cytokines on the afferent and efferent arms of the immune system in the female reproductive tract. In: Handbook of Mucosal Immunology Eds Ogra PL, Mestecky J, Lamm ME, Strober W, Mc Ghee JR, Bienestock J, Academic Press, New York.