Mastitisli İnek Sütlerinden İzole Edilen Koagulaz Negatif Stafilokokların Antibiyotik Dirençlilikleri

Year 2010, Volume: 21 Issue: 2, 107 - 111, 01.06.2010

Esra Şeker Erhan Özenç

Abstract

Keywords

-

Mastitisli İnek Sütlerinden İzole Edilen Koagulaz Negatif Stafilokokların Antibiyotik Dirençlilikleri

Abstract

Bu çalışmada mastitisli inek sütlerinden izole edilen koagulaz negatif stafilokok (KNS) türlerinin antibiyotik dirençliliklerinin belirlenmesi amaçlandı. Antibiyotik duyarlılık testleri 156 ineğe ait 620 çiğ süt örneğinden izole edilen 146 KNS izolatı üzerinde gerçekleştirildi. En yüksek direnç oranı penisilin G’ye (%74) karşıydı; bu oranı, ampisilin (%68.5), amoksisilin (%62.3), sefoksitin (%61.6), kloksasilin (%59.6) ve oksasiline (%58.9) karşı direnç oranları izledi. Test edilen 146 suşun sadece 28’i tüm antibiyotiklere duyarlı iken, geriye kalan 118 izolat en az bir antibiyotiğe dirençliydi. İzolatlardan 86’sında oksasilin direnci belirlendi. Oksasiline dirençli olan izolatların %100’ü ayrıca, ampisilin, penisilin G, sefoksitin ve kloksasiline de dirençliydi. Sonuç olarak Afyonkarahisar’da mastitisli inek sütlerinden izole edilen KNS türlerinde veteriner hekimliği alanında yaygın olarak kullanılan çok sayıda antibiyotiğe karşı direnç geliştiği belirlendi

Keywords

Antibiyotik duyarlılık testi, İnek, Koagulaz negatif stafilokok, Mastitis

References

• Akan M, Kökçü L, Öncel T, Eken HS (2001). Mastitislerden izole edilen stafilokok suşlarının beta laktamaz aktivitesi ve bazı antibiyotiklere duyarlılıklar. Vet Hek Mikrobiyol Derg, 1, 31-34.
• Archer GL, Climo MW (1994). Antimicrobial susceptibility of coagulase-negative staphylococci. Antimicrob Agents Chemother, 38 2231-2237.
• Bradley AJ (2002). Bovine mastitis: An evolving disease. Vet J, 164,116–128.
• Chaffer M, Leitner G, Winkler M, Glickman A, Krifucks O, Ezra E, Saran A (1999). Coagulase-negative staphylococci and mammary gland infections in cows. Vet Microbiol, 46, 707-712.
• Clinical and Laboratory Standards Institute (CLSI) (2006). Performance standards for antimicrobial susceptibility testing. Sixteenth Informational Supplement M1000-S16. Wayne S. PA, US.
• Danmap (2001). Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, foods and humans http://www.danmap.org/pdfFiles/Danmap_2001.pdf tarihi: 20.03.2010 Web Erişim: Erişim
• De Vliegher S, Barkema HW, Stryhn H, Opsomer G, De Kruif A (2005). Impact of early lactation somatic cell count in heifers on milk yield over the first lactation. J Dairy Sci, 88, 938-947.
• Gentilini E, Denamiel G, Betancor A, Rebuelto M, Rodriguez M, De Torrest RA (2002). Antimicrobial susceptibility of coagulase- negative staphylococci isolated from bovine mastitis in Argentina. J Dairy Sci, 85, 1913-1917.
• Gianneechini RE, Concha C, Franklin A (2002). Antimicrobial susceptibility of udder pathogens isolated from dairy herds in the west Littoral region of Uruguay. Acta Vet Scand, 43, 31-41.
• Gillespie BE, Headrick SI, Boonyayatra S, Oliver SP (2009). Prevalence and persistence of coagulase-negative Staphylococcus species in three dairy research herds. Vet Microbiol, 134, 65-72.
• Harmon RJ (1994). Symposium: Mastitis and genetic evaluation for somatic cell count. Physiology of mastitis and factors affecting somatic cell counts. J Dairy Sci, 77, 2103-2112.
• Holth JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (2000). Bergey’s Manual of Determinative Bacteriology. Lippincott Williams and Wilkins, Philadelphia.
• Jarp J (1991). Classification of coagulase-negative staphylococci isolated from bovine clinical and subclinical mastitis. Vet Microbiol, 27, 151-158.
• Khan SA, Nawaz MS, Khan AA, Steele RS, Cerniglia CE (2000). Characterization of erythromycin-resistant methylase genes from multiple antibiotic resistant Staphylococcus spp. isolated from milk samples of lactating cows. Am J Vet Res, 61, 1128-1132.
• Kırkan Ş, Göksoy EÖ, Kaya O (2005). Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydın region of Turkey. Turk J Vet Anim Sci, 29, 791-796.
• Kudinha T, Simango C (2002). Prevalence of coagulase-negative staphylococci in bovine mastitis in Zimbabwe. J S Afr Vet Assoc, 73, 62-65.
• Kuyucuoğlu Y, Hadimli HH, Kenar B, Şeker E (2003). Mastitisli ineklerden izole edilen stafilokok suşlarında beta laktamaz aktivitileri ve bazı antibiyotiklere duyarlılıkları. Vet Hek Mikrobiyol Derg, 3, 15-20.
• Li X, Mehrotra M, Ghimire S, Adewoye L (2007). β-Lactam resistance and β-lactamases in bacteria of animal origin. Vet Microbiol, 121, 197-214.
• Lüthje P, Schwarz S (2006). Antimicrobial resistance of coagulase- negative staphylococci from bovine subclinical mastitis with particular phenotypes and genotypes. J Antimicrob Chemother, 57, 966-969.
• Malinowski E, Klossowska A, Kaczmarowski M, Lassa H, Kuzma K (2002). Antimicrobial susceptibility of staphylococci isolated from affected with mastitis cows. Bull Vet Inst Pulawy, 46, 289- 294.
• Maran (2005). Monitoring of antimicrobial resistance and antibiotic usage in animals in the Netherlands in 2005. Web Erişim: http://www.cidc-lelystad.wur.nl/UK/publications/ Erişim tarihi: 20.03.2010
• Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS, Koo HC (2007). Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J Dairy Sci, 90, 1176-1185.
• Norm-Vet (2005). Usage of antimicrobial agents and occurrence of antimicrobial resistance in Norway, 2006. Web Erişim: http://www.vetinst.no/Arkiv/Zoonosesenteret/NORM_NORM- VET_2005.pdf Erişim tarihi: 23.03.2010
• Palazzo IC, Darini AL (2006). Evaluation of methods for detecting oxacillin resistance in coagulase-negative staphylococci including cefoxitin disc diffusion. FEMS Microbiol Lett, 257, 299–305.
• Pitkälä A, Haveri M, Pyörälä S, Myllys V, Honkanen-Buzalski T (2004). Bovine mastitis in Finland 2001-prevalence, distrubition of bacteria, and antimicrobial resistance. J Dairy Sci, 87, 2433- 2441.
• Pyörälä S, Taponen S (2009). Coagulase-negative staphylococci- Emerging mastitis pathogens. Vet Microbiol, 134, 3-8.
• Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002). Veterinary Microbiology and Microbial Disease. Blackwell Publishing Professional, Iowa.
• Rajala-Schultz PJ, Smith KL, Hogan JS, Love BC (2004). Antimicrobial susceptibility of mastitis pathogens from first lactation and older cows. Vet Microbiol, 102, 33-42.
• Roberson JR, Mixon J, Rohrbach B, Holland R (2006). Etiologic agents associated with high SCC dairy herds. In: Proceedings of the 24th World Buiatrics Congress, Nice, France.
• Sawant AA, Sordillo LM, Jayarao BM (2005). A survey on antibiotic usage in dairy herds in Pennsylvania. J Dairy Sci, 88, 2991-2999.
• Swenson JM, Tenover FC (2005). Cefoxitin disk study group. Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J Clin Microbiol, 43, 3818–3823.
• Taponen S, Pyörälä S (2009). Coagulase-negative staphylococci as cause of bovine mastitis-not so different from Staphylococcus aureus? Vet Microbiol, 134, 29-36.
• Taponen S, Simojoki H, Haveri M, Larsen HD, Pyörälä S (2006). Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Vet Microbiol, 115, 199-207.
• Tenhagen BA, Koster G, Wallmann J, Heuwieser W (2006). Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J Dairy Sci, 89, 2542-2551.
• Turutoglu H, Ercelik S, Ozturk D (2006). Antibiotic resistance of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. Bull Vet Inst Pulawy, 50, 41-45.