Methylene-Tetrahydrofolate Reductase 677 and 1298 Variations in Relation to Recurrent Abortion

Year 2022, , 20 - 26, 24.08.2022

Belan Kanabe , Mehmet Özaslan

https://doi.org/10.55549/zbs.1167427

Abstract

Recurrent abortion is multifactorial involving clinical and biological risk factors. Evidence addressed the relationship of inherited thrombophilia with repeated pregnancy abortion and other serious pregnancy complications. However, the relation between thrombophilia associated gene mutations and adverse obstetric outcome is controversial and data in the literature are inconsistent. Main Purpose of this research was to exanimate the prevalence of Methylene-Tetrahydrofolate Reductase gene (MTHFR) variations in association with recurrent abortion. A total of 92 samples were screened in the project: 52 women with multiple consecutive abortion and 40 normal controls. DNA of genomic was extracted from whole blood. For evaluate the presence of the both usual A1298C and C677T MTHFR gene variations in the women with recurrent abortion and controls, we employed real time polymerase chain reaction (RT-PCR). There is critical distinction in the pervasiveness of 677T/T genotype among ladies with repetitive premature birth and typical controls (P = 0.001). Consequently, the outcomes show significant difference in MTHFR C677T/A1298C genotype distribution among the healthy and women with recurrent abortion; hence, further examinations on bigger populace and other hereditary changes to more readily comprehend the molecular pathobiology of repeated pregnancy are required.

Keywords

Recurrent abortion, MTHFR 677, MTHFR 1298, RT-PCR

References

• 1. Alijotas-Reig J, Garrido-Gimenez C. Current concepts and new trends in the diagnosis and management of recurrent miscarriage. Obstet Gynecol Surv.
• 2. 2013;68(6):445–66. 2. Ural ÜM, Tekin YB, Balik G, Şahin FK, Çolak S. Could platelet distribution width be a predictive marker for unexplained recurrent miscarriage? Arch Gynecol Obstet. 2014;290(2):233–6
• 3. Makino A, Nakanishi T, Sugiura-Ogasawara M, Ozaki Y, Suzumori N, Suzumori K. No association of C677T methylenetetrahydrofolate reductase and an endothelial nitric oxide synthase polymorphism with recurrent pregnancy loss. Am J Reprod Immunol. 2004;52(1):60–6.
• 4. Vanilla S, Dayanand C, Kotur PF, Kutty MA, Vegi PK. Evidence of paternal N5, N10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in couples with recurrent spontaneous abortions (RSAs) in Kolar District—a South West of India. J Clin Diagn Res JCDR. 2015;9(2):BC15.
• 5. E. Trabetti, “Homocysteine, MTHFR gene polymorphisms, and cardio-cerebrovascular risk,” Journal of Applied Genetics, vol. 49, no. 3, pp. 267–282, 2008.
• 6. Brenner B, Sarig G, Weiner Z, Younis J, Blumenfeld Z, Lanir N. Thrombophilic polymorphisms are common in women with fetal loss without apparent cause. Thromb Haemost. 1999;82(1):6–9. 7. Ueland PM, Hustad S, Schneede J, Refsum H, Vollset SE. Biological and clinical implications of the MTHFR C677T polymorphism. Trends Pharmacol Sci. 2001;22(4):195–201.
• 8. N. Mtiraoui, W. Zammiti, L. Ghazouani et al., “Methylenetetrahydrofolate reductase C677T and A1298C polymorphism and changes in homocysteine concentrations in women with idiopathic recurrent pregnancy losses,” Reproduction, vol. 131, no. 2, pp. 395–401, 2006.
• 9. G. Unfried, A. Griesmacher, W. Weism¨uller, F. Nagele, J. C. Huber, and C. B. Tempfer, “The C677T polymorphism of the methylenetetrahydrofolate reductase gene and idiopathic recurrent miscarriage,” Obstetrics and Gynecology, vol. 99, no. 4, pp. 614–619, 2002.
• 10. C. V. Ananth, M. R. Peltier, C. De Marco et al., “Associations between 2 polymorphisms in the methylenetetrahydrofolate reductase gene and placental abruption,” American Journal of Obstetrics and Gynecology, vol. 197, no. 4, p. 385, 2007.
• 11. Van der Molen EF, Arends GE, Nelen WL, van der Put NJ, Heil SG, Eskes TK, Blom HJ. A common mutation in the 5,10-methylenetetrahydrofolate reductase gene as a new risk factor for placental vasculopathy. Am J Obstet Gynecol. 2000;182(5):1258–63.
• 12. Tiwari D, Bose PD, Das S, Das CR, Datta R, Bose S. MTHFR (C677T) polymorphism and PR (PROGINS) mutation as genetic factors for preterm delivery, fetal death and low birth weight: a Northeast Indian population based study. Meta Gene. 2015;3:31–42.