Study the effect of Urokinase plasminogen activator on the osteogenic differentiation capacity of mesenchymal stem cell

Year 2021, Volume: 2 Issue: 3, 6 - 14, 30.08.2021

Nasrın Asadı Nıkoo Nasoohı Mashıd Hodjat

Abstract

Background and Objective: Human mesenchymal stem cells (MSC) have great application in tissue engineering, specifically bone tissue engineering. There is an evidence that the receptor of urokinase plasminogen activator (uPA) involves in osteogenic differentiation of mesenchymal stem cells. The aim of this study was to identify the effect of uPA and its inhibitor on osteogenic differentiation of mesenchymal stem cells (MScs).
Methods: The human dental pulp stem cells were treated with different concentrations of uPA and its inhibitor (amiloride) in osteogenic medium. MTT assay was used to study the cell viability. The expression of osteogenic genes was assessed by quantitative real-time PCR. Alizarin red staining and alkaline phosphatase activity (Alp) were used to evaluate the osteogenic potential of MScs
Results: Amiloride at concentrations more than 0.125 mM has a toxic effect while uPA showed a significant increase in cell proliferation at 1, 2, 4, 8 nM. The alkaline phosphatase activity increased in the presence of uPA while decreased in the presence of amiloride. The data showed that cells treated with osteogenic medium in the presence of urokinase did not have a positive effect on osteogenic differentiation while amiloride decreased mineralization. Urokinase did not have a significant effect on osteopontin and osteocalcin gene expression while amiloride significantly reduced the expressions of osteogenic related genes.
Conclusion: urokinase is an essential factor in osteogenic differentiation of mesenchymal stem cells and its inhibition leads to disruption of this process, however treatment of cells with urokinase has no effect on osteogenic potential.

Keywords

urokinase plasminogen activator, Human mesenchymal stem cells, osteogenic differentiation

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