Year 2019, Volume 32, Issue 2, Pages 409 - 424 2019-06-01

Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method

Olcay GULCICEK [1] , Ali UNYAYAR [2] , Erman OZER [3] , Ozlem SANSARCI [4]

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There are many and various microorganisms earth flora, ranging from macroscopic to microscopic forms. When soil ecosystem is contaminated by complex compounds and heavy metals, which are difficult to separate from each other, in petroleum and petroleum derived products, for that reason it would be unable to utilization of soil due to decomposition of its physical, chemical and biological characteristics. Biological decomposition of petroleum and petroleum derived products by natural microorganism populations, is the primary mechanism for eliminating the petroleum and other hydrocarbon pollution of environment. The purpose of this study is to research the best isolation method on the isolation of thermophilic bacteria from the soils which are contaminated by petroleum and petroleum derived products. Recent studies related to thermophilic bacteria have been intensified since these bacteria are more resistant to extreme environmental conditions than other bacteria species. With this purpose, 4 different isolation methods have been investigated in isolating the thermophilic bacteria from the petroleum contaminated soil which is contaminated by petroleum and petroleum products. At the end of the research, in order to identify bacteria colonies which are considered to be pure, DNA isolation, Conventional PCR (polymerase chain reaction) Process, Gel Electrophoresis processes are applied.  Different incubation times, carbon resources and enrichment solutions are tried out to obtain a superior method and it is revealed that with the increase in the incubation period, more known/unknown species can be isolated without using additional carbon source from the soil.

Petroleum contaminated soil, Thermophilic bacteria, Isolation
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Primary Language en
Subjects Engineering
Journal Section Biology
Authors

Author: Olcay GULCICEK (Primary Author)
Institution: MERSIN UNIVERSITY
Country: Turkey


Author: Ali UNYAYAR
Institution: MERSIN UNIVERSITY

Author: Erman OZER
Institution: MERSIN UNIVERSITY

Author: Ozlem SANSARCI

Dates

Publication Date: June 1, 2019

Bibtex @research article { gujs457180, journal = {GAZI UNIVERSITY JOURNAL OF SCIENCE}, issn = {}, eissn = {2147-1762}, address = {Gazi University}, year = {2019}, volume = {32}, pages = {409 - 424}, doi = {}, title = {Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method}, key = {cite}, author = {GULCICEK, Olcay and UNYAYAR, Ali and OZER, Erman and SANSARCI, Ozlem} }
APA GULCICEK, O , UNYAYAR, A , OZER, E , SANSARCI, O . (2019). Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method. GAZI UNIVERSITY JOURNAL OF SCIENCE, 32 (2), 409-424. Retrieved from http://dergipark.org.tr/gujs/issue/45480/457180
MLA GULCICEK, O , UNYAYAR, A , OZER, E , SANSARCI, O . "Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method". GAZI UNIVERSITY JOURNAL OF SCIENCE 32 (2019): 409-424 <http://dergipark.org.tr/gujs/issue/45480/457180>
Chicago GULCICEK, O , UNYAYAR, A , OZER, E , SANSARCI, O . "Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method". GAZI UNIVERSITY JOURNAL OF SCIENCE 32 (2019): 409-424
RIS TY - JOUR T1 - Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method AU - Olcay GULCICEK , Ali UNYAYAR , Erman OZER , Ozlem SANSARCI Y1 - 2019 PY - 2019 N1 - DO - T2 - GAZI UNIVERSITY JOURNAL OF SCIENCE JF - Journal JO - JOR SP - 409 EP - 424 VL - 32 IS - 2 SN - -2147-1762 M3 - UR - Y2 - 2018 ER -
EndNote %0 GAZI UNIVERSITY JOURNAL OF SCIENCE Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method %A Olcay GULCICEK , Ali UNYAYAR , Erman OZER , Ozlem SANSARCI %T Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method %D 2019 %J GAZI UNIVERSITY JOURNAL OF SCIENCE %P -2147-1762 %V 32 %N 2 %R %U
ISNAD GULCICEK, Olcay , UNYAYAR, Ali , OZER, Erman , SANSARCI, Ozlem . "Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method". GAZI UNIVERSITY JOURNAL OF SCIENCE 32 / 2 (June 2019): 409-424.
AMA GULCICEK O , UNYAYAR A , OZER E , SANSARCI O . Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method. GAZI UNIVERSITY JOURNAL OF SCIENCE. 2019; 32(2): 409-424.
Vancouver GULCICEK O , UNYAYAR A , OZER E , SANSARCI O . Thermophilic Bacteria Investigation in Petroleum Contaminated Soils by Different Isolation Method. GAZI UNIVERSITY JOURNAL OF SCIENCE. 2019; 32(2): 424-409.