EXPERIMENTAL INVESTIGATION OF THE EFFECTS OF PROBIOTIC SUPPLEMENTATION ON OBESITY AND INFLAMMATION IN OBESE RATS

Year 2017, Volume: 2 Issue: 2, 44 - 50, 22.12.2017

Gül Eda Kılınç , Mehtap Ünlü Söğüt

https://doi.org/10.23884/ijhsrp.2017.2.2.02

Abstract

TNF-α levels
of antiinflammatory and proinflammatory markers were used to investigate
effects Obesity is a disease that occurs when body
has many endocrine and metabolic functions and fat tissue is higher than
normal. There is strong relationship between obesity and inflammation due to
production of numerous lipid molecules called adipokines, such as leptin, tumor
necrosis factor (TNF-α), interleukins and adiponectin. It was aimed to study
obesity and effect on inflammation in experimentally obese animal model study
apllied probiotic supplementation. Three different test animal groups
were formed. For this purpose; IL-10 and TNF-α on inflammation; leptin levels
were used for investigating effect on development of obesity and total
cholesterol, HDL and LDL levels from lipid profiles were planned to examine. In
obtained serum samples, these parameters were determined by sandwich ELISA
technique with commercially available kits.  Mean weights at the beginning of study
and at the end of 8 weeks were 240,2±9,7 and 283,8±11,0  gr in first, second and third groups;
254,6±16,0 and 307,0±14,5 gr; 330,0±48,6 and 400,5±65,0 gr respectively. At the
beginning and end of the probiotic use, weight averages were 288,6±9,9 and
311,8±17,1 gr in first, second and third groups 313.4±17.1 and 339.6±19.7 gr;
412,0±67,7 and 422,0±71,1 gr respectively. When weights were evaluated before
and after probiotic use of third group, weight gain was decreased. Although
there was no significant difference in HDL, LDL, IL-10 and leptin levels among
all group, there was significant difference in total cholesterol and TNF-α
values (p<0,05). Compared to first group and second group, there was significant
difference in total cholesterol and TNF-α values, in second and third groups
there was significant difference in total cholesterol values (p<0,05). As result; probiotics are recognized as living
organisms when taken in sufficient quantities, affect health of environment
positively, and these beneficial effects are considered to extend to obesity
inflammatory diseases. Probiotic supplementation of normal diet may be healthy
approach to prevention of various diseases. In order to spread this application
to society as whole, it is necessary to increase number of works.

Keywords

obesity, inflammation, probiotic

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