DOI: 10.26650/EurJBiol.2018.400108
Objective: The electrophoretic profile of
serum proteins was investigated in juvenile Piaractus mesopotamicus and
Salminus brasiliensis fed with diets containing bovine colostrum, a
nutraceutical food, in lyophilized form (LBC) for either 30 or 60 days.
Materials and Methods: Blood samples were
collected from juveniles of P. mesopotamicus and S. brasiliensis fed for either
30 or 60 days with diets containing 0%, 10% or 20% of LBC. Serum protein
fractions were then determined by electrophoresis in agarose gel.
Results: The P. mesopotamicus, an
omnivorous fish, showed six serum protein fractions, while the S. brasiliensis,
a carnivorous fish, showed four serum protein fractions. In both species the
albumin fraction showed higher protein content at 30 days than at 60 days (p<0.05),
indicating an inverse relationship with growth. The 5th and 4th fraction in
mobility from the P. mesopotamicus and S. brasiliensis serum, respectively,
were positioned in a gamma-globulin zone. In P. mesopotamicus, the
concentration of protein in the gamma-globulin zone was higher at 60 days than
at 30 days (p<0.05). In S. brasiliensis, in turn, the concentration of
protein in the gamma-globulin zone was higher at 30 days than at 60 days
(p<0.05). In juvenile P. mesopotamicus, a higher concentration of protein
was also observed in the gamma-globulin zone in the 0% LBC compared to 10% and
20% LBC (p<0.05). Thus, the feeding period influenced only the fractions
that were positioned in the albumin and gamma-globulin migration zone in both
species.
Conclusion: The consumption of bovine
colostrum decreased the concentration of protein in gamma-globulin fraction of
the P. mesopotamicus, indicating that these juveniles had either less stimulus
for their own synthesis of defense elements or an immunosuppressive effect of
bovine colostrum ingestion.
Birincil Dil | İngilizce |
---|---|
Bölüm | Araştırma Makaleleri |
Yazarlar | |
Yayımlanma Tarihi | 2 Aralık 2018 |
Gönderilme Tarihi | 1 Mart 2018 |
Yayımlandığı Sayı | Yıl 2018 Cilt: 77 Sayı: 2 |