Year 2018, Volume 1, Issue 3, Pages 35 - 35 2018-12-08

Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity

Samya ALENEZI [1] , Naif Alenezi MANAL [2] , J NATTO [3] , Harry P. De KONING [4] , John IGOLI [5] , David G. WATSON [6]

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This study is aimed to investigate the chemical profile and examine the in vitro efficacy of Papua new guinea (PNG) propolis and compounds purified from it purified compounds against the protozoal parasites: Crithidia fasciculate, Trypansoma congolense, Trypanosoma b. brucei and drug resistant Trypanosoma brucei (B48), which cause diseases in humans and other animals as well as insects. In addition. The samples were assayed for their toxicity against human U937 cells in vitro.

            Chemical profiling was conducted by using negative ion spray ESI (LC-MS) and indicated that the ethanolic extract of PNG contained a series of triterpenes. Principle components analysis (PCA) of the processed LC-MS data collected was demonstrated the uniqueness in chemical composition of the fractions prepared from the sample that were also active against the parasites. Active principles were isolated by bioassay-led fractionation, using medium pressure chromatographic (MPLC). Afterwards, by using spectroscopic and spectrometric means, nine pure compounds were isolated and the their structures were elucidated indicating that eight of them were cycloartane type triterpenes. While the other one was a pentacyclic triterpene, 20 hydroxy betuline that was found to be the most active compound in these assays. Growth curves of drug-resistant T. brucei at concentrations ≥ EC50 showed that this compound was trypanostatic. Overall, the PNG propolis and its fractions showed low toxicity on U937 cell growth and were efficacious against kinetoplastid parasites. 
Papua New Guinea Propolis, Chemical Profiling, Antiprotozoal Activity
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Primary Language en
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: Samya ALENEZI (Primary Author)

Author: Naif Alenezi MANAL

Author: J NATTO

Author: Harry P. De KONING

Author: John IGOLI

Author: David G. WATSON

Dates

Publication Date: December 8, 2018

Bibtex @conference paper { jan489270, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {35 - 35}, doi = {}, title = {Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity}, key = {cite}, author = {ALENEZI, Samya and MANAL, Naif Alenezi and NATTO, J and KONING, Harry P. De and IGOLI, John and WATSON, David G.} }
APA ALENEZI, S , MANAL, N , NATTO, J , KONING, H , IGOLI, J , WATSON, D . (2018). Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity. Journal of Apitherapy and Nature, 1 (3), 35-35. Retrieved from http://dergipark.org.tr/jan/issue/40577/489270
MLA ALENEZI, S , MANAL, N , NATTO, J , KONING, H , IGOLI, J , WATSON, D . "Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity". Journal of Apitherapy and Nature 1 (2018): 35-35 <http://dergipark.org.tr/jan/issue/40577/489270>
Chicago ALENEZI, S , MANAL, N , NATTO, J , KONING, H , IGOLI, J , WATSON, D . "Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity". Journal of Apitherapy and Nature 1 (2018): 35-35
RIS TY - JOUR T1 - Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity AU - Samya ALENEZI , Naif Alenezi MANAL , J NATTO , Harry P. De KONING , John IGOLI , David G. WATSON Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 35 EP - 35 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity %A Samya ALENEZI , Naif Alenezi MANAL , J NATTO , Harry P. De KONING , John IGOLI , David G. WATSON %T Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD ALENEZI, Samya , MANAL, Naif Alenezi , NATTO, J , KONING, Harry P. De , IGOLI, John , WATSON, David G. . "Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity". Journal of Apitherapy and Nature 1 / 3 (December 2018): 35-35.
AMA ALENEZI S , MANAL N , NATTO J , KONING H , IGOLI J , WATSON D . Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity. J.Apit.Nat.. 2018; 1(3): 35-35.
Vancouver ALENEZI S , MANAL N , NATTO J , KONING H , IGOLI J , WATSON D . Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity. Journal of Apitherapy and Nature. 2018; 1(3): 35-35.