Year 2018, Volume 1, Issue 3, Pages 68 - 68 2018-12-08

Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity

G.A. DOS SANTOS [1] , A.A. LAGAREIRO NETTO [2] , P.B. MENDES [3] , V. NEGRÃO [4] , M.C. MARCUCCI [5] , S.N. DINIZ [6] , M.J. CARNEIRO [7] , A.C.H.F. SAWAYA [8]

17 101

Propolis, a resin produced by A. mellifera bees, presents several compounds of pharmaceutical interest and, further studies to demonstrate their pharmacological actions is sought. Capsules containing green propolis (BRPX) extracted in flax oil (1 and 2) polyethylene glycol (PEG) (3 and 4) were analyzed and analyzed according to IN 31. The following parameters were evaluated: total phenols and flavonoids, quantification of Artepillin-C (HPLC) confirmed by UHPLC-MS, antitumor activity in MCF-7 (breast code 0162) and B16F10 (melanoma code 0046) and antimicrobial activity (E.coli, C. albicans and S .aureus). The flavonoid content (%) of each capsule was 1: 1.135 ± 0.009, 2: 0.521 ± 0.039, 3: 0.758 ± 0.020, 4: 1.232 ± 0.09. The phenol content (%) of 1: 2,509 ± 0,024, 2: 2,280 ± 0,075, 3: 1,039 ± 0,029, 4: 3,426 ± 0,074. The content of Artepillin-C (mg / capsule) of 1: 1.39 ± 0.12, 2: 1.07 ± 0.05, 3: 0.66 ± 0.01, 4: 0.39 ± 0, 01. The IC50 (μg / mL) for MCF-7 was: 1: 100.49 ± 6.41, 2: 463.65 ± 0.71, 3: 209.03 ± 35.99, 4: 238.84 ± 4 , 16 and for B16F10, 1: 277.53 ± 7.37, 2: 357.48 ± 10.15, 3: 376.15 ± 5.92, 4: 286.87 ± 10.79. Inhibition halos ranged from 10 to 14 mm (E.coli), 8 to 10 mm (C. albicans) and 8 to 9 mm (S.aureus). This study demonstrates the importance of quality control for reliable formulations based on propolis.
A. mellifera, Propolis, HPLC
  • 1. BRASIL (2001). MINISTRY OF AGRICULTURE. Normative Instruction nº 3 - ANNEX VII - Technical regulation for determination of identity and quality of propolis. Diário Oficial da República Federativa do Brasil.
Primary Language en
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: G.A. DOS SANTOS

Author: A.A. LAGAREIRO NETTO

Author: P.B. MENDES

Author: V. NEGRÃO

Author: M.C. MARCUCCI

Author: S.N. DINIZ

Author: M.J. CARNEIRO

Author: A.C.H.F. SAWAYA

Dates

Publication Date: December 8, 2018

Bibtex @conference paper { jan489880, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {68 - 68}, doi = {}, title = {Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity}, key = {cite}, author = {DOS SANTOS, G.A. and LAGAREIRO NETTO, A.A. and MENDES, P.B. and NEGRÃO, V. and MARCUCCI, M.C. and DINIZ, S.N. and CARNEIRO, M.J. and SAWAYA, A.C.H.F.} }
APA DOS SANTOS, G , LAGAREIRO NETTO, A , MENDES, P , NEGRÃO, V , MARCUCCI, M , DINIZ, S , CARNEIRO, M , SAWAYA, A . (2018). Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity. Journal of Apitherapy and Nature, 1 (3), 68-68. Retrieved from http://dergipark.org.tr/jan/issue/40577/489880
MLA DOS SANTOS, G , LAGAREIRO NETTO, A , MENDES, P , NEGRÃO, V , MARCUCCI, M , DINIZ, S , CARNEIRO, M , SAWAYA, A . "Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity". Journal of Apitherapy and Nature 1 (2018): 68-68 <http://dergipark.org.tr/jan/issue/40577/489880>
Chicago DOS SANTOS, G , LAGAREIRO NETTO, A , MENDES, P , NEGRÃO, V , MARCUCCI, M , DINIZ, S , CARNEIRO, M , SAWAYA, A . "Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity". Journal of Apitherapy and Nature 1 (2018): 68-68
RIS TY - JOUR T1 - Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity AU - G.A. DOS SANTOS , A.A. LAGAREIRO NETTO , P.B. MENDES , V. NEGRÃO , M.C. MARCUCCI , S.N. DINIZ , M.J. CARNEIRO , A.C.H.F. SAWAYA Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 68 EP - 68 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity %A G.A. DOS SANTOS , A.A. LAGAREIRO NETTO , P.B. MENDES , V. NEGRÃO , M.C. MARCUCCI , S.N. DINIZ , M.J. CARNEIRO , A.C.H.F. SAWAYA %T Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD DOS SANTOS, G.A. , LAGAREIRO NETTO, A.A. , MENDES, P.B. , NEGRÃO, V. , MARCUCCI, M.C. , DINIZ, S.N. , CARNEIRO, M.J. , SAWAYA, A.C.H.F. . "Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity". Journal of Apitherapy and Nature 1 / 3 (December 2018): 68-68.
AMA DOS SANTOS G , LAGAREIRO NETTO A , MENDES P , NEGRÃO V , MARCUCCI M , DINIZ S , CARNEIRO M , SAWAYA A . Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity. J.Apit.Nat.. 2018; 1(3): 68-68.
Vancouver DOS SANTOS G , LAGAREIRO NETTO A , MENDES P , NEGRÃO V , MARCUCCI M , DINIZ S , CARNEIRO M , SAWAYA A . Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity. Journal of Apitherapy and Nature. 2018; 1(3): 68-68.