Year 2018, Volume 1, Issue 3, Pages 77 - 77 2018-12-08

Antituberculosis Activity of Propolis

Jarosław WIDELSKI [1] , Joanna GOLUS [2] , Piotr OKIŃCZYC [3] , Rafał SAWICKI [4] , Grażyna GINALSKA [5] , Tomasz MROCZEK [6] , Zuriyadda SAKIPOVA [7] , Ioanna CHINOU [8] , Krystyna SKALICKA-WOŹNIAK [9]

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Tuberculosis an infectious disease caused by Mycobacterium tuberculosis is one of the leading causes of human morbidity and mortality. In 2013 the WHO reported one and half million of deaths and nine millions new cases of active tuberculosis caused by TB1.The increase in the incidence of clinical tuberculosis is associated with increasing reports of new cases of multi drug resistant (MDR-TB) and extensively multidrug resistant (XDR-TB) strains1.

Propolis (bee glue) is well known for its antibacterial and antifungal properties.More than 30 ethanol-water extracts obtained from different propolis samples were tested for antituberculosis activity.

Minimal Inhibitory Concentrations (MIC) values for the EO were established with 96-well micro plate method with AlamarBlue (Invitrogen). Reference strain of Mycobacterium tuberculosis H37Ra inoculum in Middlebrook 7H9 broth (Difco) was 5x105cfu/ml per well, accordingly to CLSI standards. Serial twofold dilutions of propolis extracts ranged from 64 to 8 µg/ml. As the internal control of the method serial twofold dilutions of four first line antibiotics dedicated to the tuberculosis treatment: isoniazid (INH), rifampicin (RMP), ethambutol (EMB) and streptomicin (SM) were used2,3.

Acknowledgments. The work was financed from grant No 4/POLTUR-1/2016.
Propolis, Mycobacterium tuberculosis, MIC
  • 1. WHO (2014). Global tuberculosis report (www.who.int/tdr/news/2014/global-TB-report/en/).World Health Organization (2010). Multidrug and extensively drug resistant TB (M/ XDR-TB) Global report on surveillance and response. Geneva: World Health
  • 2 Palomino, J.C., Martin, A., Camacho, A., Guerra, H., Swings, J. ,Portaels, F.(2002). Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotheraphy, 46, 2720-2722
  • 3 Wayene, P.A. (2011). Susceptibility Testing of Mycobacteria, Nocarediae, and Other Aerobic Acinomycetes; Approved Standard-Second Edition. CLSI document M24-A2. USA. Clinical and Laboratory Standards Institute.
Primary Language en
Subjects
Journal Section 2nd Propolis Conference Special Issue
Authors

Author: Jarosław WIDELSKI

Author: Joanna GOLUS

Author: Piotr OKIŃCZYC

Author: Rafał SAWICKI

Author: Grażyna GINALSKA

Author: Tomasz MROCZEK

Author: Zuriyadda SAKIPOVA

Author: Ioanna CHINOU

Author: Krystyna SKALICKA-WOŹNIAK

Bibtex @conference paper { jan489986, journal = {Journal of Apitherapy and Nature}, issn = {}, eissn = {2667-4734}, address = {Oktay YILDIZ}, year = {2018}, volume = {1}, pages = {77 - 77}, doi = {}, title = {Antituberculosis Activity of Propolis}, key = {cite}, author = {WIDELSKI, Jarosław and GOLUS, Joanna and OKIŃCZYC, Piotr and SAWICKI, Rafał and GINALSKA, Grażyna and MROCZEK, Tomasz and SAKIPOVA, Zuriyadda and CHINOU, Ioanna and SKALICKA-WOŹNIAK, Krystyna} }
APA WIDELSKI, J , GOLUS, J , OKIŃCZYC, P , SAWICKI, R , GINALSKA, G , MROCZEK, T , SAKIPOVA, Z , CHINOU, I , SKALICKA-WOŹNIAK, K . (2018). Antituberculosis Activity of Propolis. Journal of Apitherapy and Nature, 1 (3), 77-77. Retrieved from http://dergipark.org.tr/jan/issue/40577/489986
MLA WIDELSKI, J , GOLUS, J , OKIŃCZYC, P , SAWICKI, R , GINALSKA, G , MROCZEK, T , SAKIPOVA, Z , CHINOU, I , SKALICKA-WOŹNIAK, K . "Antituberculosis Activity of Propolis". Journal of Apitherapy and Nature 1 (2018): 77-77 <http://dergipark.org.tr/jan/issue/40577/489986>
Chicago WIDELSKI, J , GOLUS, J , OKIŃCZYC, P , SAWICKI, R , GINALSKA, G , MROCZEK, T , SAKIPOVA, Z , CHINOU, I , SKALICKA-WOŹNIAK, K . "Antituberculosis Activity of Propolis". Journal of Apitherapy and Nature 1 (2018): 77-77
RIS TY - JOUR T1 - Antituberculosis Activity of Propolis AU - Jarosław WIDELSKI , Joanna GOLUS , Piotr OKIŃCZYC , Rafał SAWICKI , Grażyna GINALSKA , Tomasz MROCZEK , Zuriyadda SAKIPOVA , Ioanna CHINOU , Krystyna SKALICKA-WOŹNIAK Y1 - 2018 PY - 2018 N1 - DO - T2 - Journal of Apitherapy and Nature JF - Journal JO - JOR SP - 77 EP - 77 VL - 1 IS - 3 SN - -2667-4734 M3 - UR - Y2 - 2019 ER -
EndNote %0 Journal of Apitherapy and Nature Antituberculosis Activity of Propolis %A Jarosław WIDELSKI , Joanna GOLUS , Piotr OKIŃCZYC , Rafał SAWICKI , Grażyna GINALSKA , Tomasz MROCZEK , Zuriyadda SAKIPOVA , Ioanna CHINOU , Krystyna SKALICKA-WOŹNIAK %T Antituberculosis Activity of Propolis %D 2018 %J Journal of Apitherapy and Nature %P -2667-4734 %V 1 %N 3 %R %U
ISNAD WIDELSKI, Jarosław , GOLUS, Joanna , OKIŃCZYC, Piotr , SAWICKI, Rafał , GINALSKA, Grażyna , MROCZEK, Tomasz , SAKIPOVA, Zuriyadda , CHINOU, Ioanna , SKALICKA-WOŹNIAK, Krystyna . "Antituberculosis Activity of Propolis". Journal of Apitherapy and Nature 1 / 3 (December 2018): 77-77.