Year 2019, Volume 32, Issue 1, Pages 20 - 26 2019-01-29

Kolinerjik bileşiklerin ve TNF-alfanın insan eritrolösemi K562 hücre çoğalmasına ve kaspaz ekspresyonu üzerine etkileri
Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression

Zehra KANLI [1] , Banu AYDIN [2] , Hulya CABADAK [3]

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Amaç: Bu çalışmanın amacı, otoparakrin M3 muskarinik
reseptör sinyal yolağının uyarılmasının, insan eritrolösemi K562
hücrelerinin çoğalmasında ve kaspaz 3, 8 ve 9 ekspresyon seviyeleri
üzerinde etkisi olup olmadığını araştırmaktır. Hücre sinyal
ileti mekanizmasında muskarinik reseptörlerin rolünü daha iyi
anlamak üzere, çeşitli bileşiklerin insan eritrolösemisi K562 hücre
proliferasyonu ve kaspaz 3, 8 ve 9 ekspresyon seviyeleri üzerindeki
etkilerini araştırdık. Bu bileşikler, M3 muskarinik reseptör agonisti,
pilokarpin, pro-enflamatuvar sitokin, tümör nekroz faktör (TNF)-
alfa ve fosfoinositid 3-kinaz inhibitörü wortmanindir.
Gereçler ve Yöntemler: Hücre çoğalması ve hücre canlılığı,
tripan mavisi testi ve 5-Bromo-2-deoxy-uridine (BrdU) İşaretleme
ve Belirleme kitleri ile değerlendirildi. Kaspaz 3, 8 ve 9 ekspresyon
seviyeleri immunoblot analizi ile belirlendi.
Bulgular: Hem pilokarpin, hem de TNF-alfa, insan eritrolösemi
K562 hücre çoğalmasında çok az artışa neden oldu. Ancak, tüm
bileşikler birlikte muamele edildiğinde, insan eritrolösemi K562
hücrelerinin çoğalması, muamele edilmeyen kontrol hücrelerine
göre anlamlı olarak arttı. TNF-alfa ve wortmanin ile muamele
edilen K562 hücrelerinde kaspaz 3 ve kaspaz 8 ekspresyonu
seviyelerinde anlamlı değişim belirlendi. TNF-alfa ve wortmannin
muamelesi kaspaz 9 ekspresyon seviyesini arttırdı (P> 0.05) ancak
anlamlı değildi.
Sonuç: Bu bulgular, kısmen M3 muskarinik reseptör aracılı
K562 hücre çoğalmasında artış olduğunu göstermektedir.
Pilokarpin, insan eritrolösemi K562 hücrelerinde, TNF-alfa ve
wortmanin ile uyarılan kaspaz 3 ve 8 ekspresyonunu önledi ve
dolaylı olarak apoptozu gösterdi.
Objective: The purpose of this study was to investigate if
stimulating auto-paracrine muscarinic receptor signalling pathway
could change human erythroleukemia K562 cell proliferation and
caspase 3, 8 and 9 expression levels. To better understand the role of
muscarinic receptors in cell signalling mechanism, we investigated
the effects of several compounds on human erythroleukemia
K562 cell proliferation and caspase 3, 8 and 9 expression. These
compounds were M3 muscarinic receptor agonist, pilocarpine, proinflammatory
cytokine, tumor necrosis factor (TNF)-alpha, and
the wortmannin which is a phosphoinositide 3-kinase inhibitor.
Materials and Methods: Cell proliferation and cell viability
were evaluated by the trypan blue exclusion test and 5-Bromo-2-
deoxy-uridine (BrdU) Labelling and Detection Kits. Caspase 3, 8
and 9 expression levels were determined by immunoblot analysis.
Results: Both pilocarpine and TNF-alpha caused a small increase
in human erythroleukemia K562 cell proliferation. However, when
all the compounds were treated together, proliferation of human
erythroleukemia K562 cells increased significantly when compared to
untreated control cells. TNF-alpha and wortmannin treatment increased
caspase 3 and caspase 8 expression patterns significantly in human
erythroleukemia K562 cells. TNF-alpha and wortmannin treatment
increased caspase 9 expression level (P>0.05) but it was not significant.
Conclusion: These findings partly demonstrated that M3
muscarinic receptor mediated an increase in K562 cell proliferation.
Pilocarpine prevented TNF-alpha and wortmannin induced
caspase 3 and 8 expression and indirectly showed apoptosis in
human erythroleukemia K562 cells.
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Primary Language en
Subjects Medicine
Journal Section Articles
Authors

Author: Zehra KANLI

Author: Banu AYDIN

Author: Hulya CABADAK (Primary Author)

Dates

Publication Date: January 29, 2019

Bibtex @research article { marumj518797, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {Marmara University}, year = {2019}, volume = {32}, pages = {20 - 26}, doi = {10.5472/marumj.518797}, title = {Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression}, key = {cite}, author = {KANLI, Zehra and AYDIN, Banu and CABADAK, Hulya} }
APA KANLI, Z , AYDIN, B , CABADAK, H . (2019). Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression. Marmara Medical Journal, 32 (1), 20-26. DOI: 10.5472/marumj.518797
MLA KANLI, Z , AYDIN, B , CABADAK, H . "Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression". Marmara Medical Journal 32 (2019): 20-26 <http://dergipark.org.tr/marumj/issue/42918/518797>
Chicago KANLI, Z , AYDIN, B , CABADAK, H . "Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression". Marmara Medical Journal 32 (2019): 20-26
RIS TY - JOUR T1 - Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression AU - Zehra KANLI , Banu AYDIN , Hulya CABADAK Y1 - 2019 PY - 2019 N1 - doi: 10.5472/marumj.518797 DO - 10.5472/marumj.518797 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 20 EP - 26 VL - 32 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.518797 UR - https://doi.org/10.5472/marumj.518797 Y2 - 2018 ER -
EndNote %0 Marmara Medical Journal Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression %A Zehra KANLI , Banu AYDIN , Hulya CABADAK %T Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression %D 2019 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 32 %N 1 %R doi: 10.5472/marumj.518797 %U 10.5472/marumj.518797
ISNAD KANLI, Zehra , AYDIN, Banu , CABADAK, Hulya . "Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression". Marmara Medical Journal 32 / 1 (January 2019): 20-26. https://doi.org/10.5472/marumj.518797
AMA KANLI Z , AYDIN B , CABADAK H . Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression. Marmara Med J. 2019; 32(1): 20-26.
Vancouver KANLI Z , AYDIN B , CABADAK H . Effects of cholinergic compounds and TNF-alpha on human erythroleukemia K562 cell proliferation and caspase expression. Marmara Medical Journal. 2019; 32(1): 26-20.