Deneysel Kronik Pankreatitte N-Asetil Sistein Uygulamasinin Pankreatik Fibroz Üzerine Etkisi

Yıl 2019, Cilt: 6 Sayı: 1, 26 - 32, 20.03.2019

Ramazan Ocal İlker Tascı Mehmet Salih Devecı Bilgin Comert Ahmet Turan Isık Mehmet Refik Mas

Öz

Bu çalışmada,
N-asetilsisteinin, kronik pankreatit hayvan modelinde fibrozis, atrofi ve
oksidatif stres üzerindeki etkisini değerlendirmeyi amaçladık. Kronik
pankreatit, ratların ana pankreatik kanallarına 0.4 ml, %2’lik trinitrobenzen
sülfanik asit (TNBS) enjeksiyonu ile uyarılır. 60 Sprague-Dawley sıçanı dört
gruba randomize edildi; Grup I (n= 15): salin (sham), Grup II (n= 15): fibrozis
+ N-asetilsistein (NAC), Grup III (n= 15): fibrozis (TNBS), Grup IV: etanol. 8.
haftanın sonunda tüm ratlar sakrefiye edildi ve pankreatik dokular, oksidatif
stres belirteçleri, fibrozis ve atrofi progresyonları incelendi. NAC grubunda
doku malondialdehid (MDA) düzeyleri anlamlı olarak düşük bulunurken (p <0.001);
süperoksit dismutaz (SOD) ve glutation peroksidaz (GSH-Px) aktiviteleri TNBS
grubuna göre anlamlı olarak yüksek bulundu (sırasıyla p <0.001 ve p <0.001).
Dokulardaki histopatolojik incelemede NAC grubunun histopatolojik skorunun TNBS
grubundan anlamlı derecede düşük olduğu izlenirken (p<0.003) oksidatif stres
ile histopatolojik skordaki artış arasında anlamlı bir ilişki olduğu saptandı
(histopatolojik skor/MDA: p<0.03, SOD: p<0.04, GSH-Px: p<0.02). Sonuç
olarak, NAC uygulaması kronik pankreatitte oksidatif stresi azaltmakta olup
fibrozis ve atrofi üzerinde yararlı etkilere sahiptir.

Anahtar Kelimeler

Deneysel Kronik Pankreatit, Fibrozis, Hayvan Modeli, N-Asetilsistein

The Effect of N-Acetylcysteine on Fibrosis in Experimental Rat Chronic Pancreatitis

Öz

In this study, we
aimed to assess the effect of N-acetylcysteine on fibrosis, atrophy and
oxidative stress with chronic pancreatitis in an animal model. Chronic
pancreatitis is stimulated by injection of 0.4 ml, 2% trinitrobenzene sulfanic
acid (TNBS) into major pancreatic ducts of the rats. 60 Sprague-Dawley rats
were randomized into four group; Group I (n=15): saline (control), Grup II
(n=15): fibrosis + N-acetylcysteine (NAC), Grup III (n=15): fibrosis (TNBS),
Grup IV: ethanol. At the end of the 8th week, all rats were sacrificed, and rat
pancreatic tissues, oxidative stress markers and progression of fibrosis and
atrophy were examined. superoxide dismutase (SOD) and glutation peroxidase
(GSH-Px) activities were significantly higher with respect to TNBS group
(respectively p<0.001 and p<0.001); whereas tissue malondialdehyde (MDA)
levels were found significantly lower in NAC group (p<0.001).  Histopathological analysis of the tissues
revealed that histopathological score of NAC group was significantly lower than
TNBS group (p<0.003), and was found a significant relation between increase
in oxidative stress and histopathological score (histopathological score/MDA:
p<0.03, SOD: p<0.04, GSH-Px: p<0,02). In conclusion, administration of
NAC decreases oxidative stress in chronic pancreatitis and has beneficial
effects on fibrosis and atrophy.

Anahtar Kelimeler

Animal Model, Experimental Chronic Pancreatitis, Fibrosis, N-Acetylcysteine

Kaynakça

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