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The Effect Of Different Workout Programs On The Expression Of The Genes Related To Oxidative Stress And Immune System

Yıl 2020, Cilt: 3 Sayı: 1, 40 - 46, 26.06.2020

Neşe Akpınar Kocakulak Serpi Taheri Elif Funda Şener Kenan Aycan Yusuf Özkul

https://doi.org/10.38061/idunas.726846

Öz

Background: exercise conditions can affect the expression of genes. Our study investigated the effects of acute and chronic exercise on the expression of genes related to oxidative stress (Tfam,UCP2,UCP3 ) and immune system (IL-1α,IL-1β,IL-2,IL-8,IL12A,IL12B,TLR2,TLR4 ).
Methods: was formed with 24 people: 12 healthy females and 12 healthy males. Maximal oxygen use capacities of the participants were determined by the Bruce test protocol at the beginning and end of the 8-week training program. After calculating their maximal oxygen use capacity, each participant was given a acute running exercise on the tread mill at the speed and incline that the participant would reach to his/her maxVO2 until exhausted. The same people was built to continuous runs (%50-70) for 8 weeks in a week, and two days of medium-term interval training program (%90-95). Peripheral blood samples were taken before and after acute exercise with immediately after chronic exercises. RNA isolation was performed using TRIzol Reagent from peripheral blood mononuclear cells. Gene expression was determined by Biomark Real-Time PCR (RT-PCR). Comparisons were performed by using two independent sample t-test and Mann-Whitney U for quantitative data and calculated gene expression values. The statistical significance level was taken as p <0,05. Results: we found that acute exercise in women changed Tfam gene expression (p <0,05 ). Chronic exercise changes the expression level of more genes (Tfam, IL-1β, TLR4 ) in women (p<0.05,p<0.001). There was only difference in Tfam gene in males (p <0,05 ). Conclusions: changes in gene expression differed by sex in exercise. Our results indicate that different workout programs for females and males cause genes to work and they have a positive effect on their expressions and thus increase the efficiency of the exercise.

Anahtar Kelimeler

exercise gene expression immune system Oxidative stress

Destekleyen Kurum

Erciyes University Scientific Research Projects Units

Proje Numarası

TSD-12-3929

Kaynakça

  • 1. Ntanasis, S, J., Tzanninis, J.G., Philippou, A.A., et al. (2013). Epigenetic regulation on gene expression induced by physical exercise. JMusculoskelet Neuronal Interact, 13, 133-146.
  • 2. Meier P, Renga M, Hoppeler H and Baum O . (2013).The impact of antioxidant supplements and endurance exercise on genes of the carbohydrate and lipid metabolism in skeletal muscle of mice. Cell Biochem Funct, 31, 51–59.
  • 3. Bangi, G., Delfabbro, M,. Maurş, C., Corsi, M., Melegati, G. (2000). Haematological parameters in elite rugby players during a competitive season. Clin.Lab.Haem, 28,183-188.
  • 4. Eynon, N., Meckel, Y., Alves, A.J., Yamin, C., Sagiv, M., et al.(2009). Is there an interaction between PPARD T294C and PPARGC1A gly482 Ser polymorphisms and human endurance performance? Exp physiol, 11,1147-1152.
  • 5. De Nadal, E., Ammerer, G., Posas, F. (2011). Controlling gene expression in response to stress. Nat Rev Genet, 12, 833–845.
  • 6. Flynn, MG., McFarlin BK., Phillips, MD., Stewart,LK., Timmerman, KL.(2003). Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise-trained elderly women, J Appl Physiol, 95(5), 1833–1842.
  • 7. Kumar, H., Kawai, T., Akira,S. (2009) Toll-like receptors and innate immunity. Biochem Biophys Res Commun, 388(4),621-5.
  • 8. Lancaster, GI., Khan, Q., Drysdale, P., et al.(2005). The physiological regulation of toll-like receptor expression and function in humans. J Physiol, 563, 945-955,
  • 9.Kocakulak,NA.,Hamurcu,Z.,Donmez-Altuntas, H., Sungur, G., Koca,F., Çoksevim,B.(2020). Effects of Exercise Performed at High Altitude on the Chromosomal DNA Damage in Human Peripheral Lymphocytes. Hacettepe J. Biol. & Chem, 48 (1), 13-20
  • 10. Kocakulak,NA.(2019). The Effect of Interval Training Program on Nuclear Factor Erythroid-Derived 2-like 2 (NFE2L2/Nrf2) Gene Expression in Women. Hacettepe J. Biol. & Chem, 47 (4), 410-414
  • 11. Gleeson, M. (2007). Immune function in sport and exercise. J Appl Physiol, 103,693-699.
  • 12. Borrs, C., Sastre, J., Garcfa-Sala, D., et al.(2003). Mitochond riafrom females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radic Biol Med, 34,546-552.
  • 13.Nilsson, C., Aboud, S., Karlen, K., et al.(2008). Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immunospot testing of healthy Swedish and Tanzanian subjects. Clin Vaccine Immunol,15, 585–9.
  • 14. Bayram, F., Diri, H., Sener, EF., Dundar,M., Simsek,Y.(2016). Genetic expressions of thrombophilic factors in patients with Sheehan’s syndrome. Gynecol Endocrinol,32(11), 908–911
  • 15. Jemiolo, B., Trappe,S. (2004). Single muscle fiber gene expression in human skeletal muscle: validation of internal contro lwith exercise,Biochem. Biophys. Res. Commun, 320(3),1043–1050.
  • 16. Catoire, M., Mensink, M., Boekschoten, MV., et al.(2012) Pronounced effects of acute endurance exercise on gene expression in resting and exercising human skeletal muscle, PloS One,7 (11) , e51066.
  • 17. Ahmetova, II., Popova, DV., Missinaa, SS., et al.(2010). Association of Mitochondrial Transcription Factor (TFAM) Gene Polymorphism with Physical Performance in Athletes. Human Physiology, 36,229–233.
  • 18. Parise, G., Brose, AN., Tarnopolsky, MA. (2005). Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Exp Gerontol,40, 173-80.
  • 19. Moldoveanu, AI., Shephard, RJ., Shek, PN.(2000). Exercise elevates plasma levels but not gene expression of IL-1b, IL-6, and TNF-a in blood mononuclear cells. J Appl Physiolog, 89,1499-1504.
  • 20. Flynn, MG., McFarlin, BK., Phillips, MD., et al.(2003). Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise-trained elderly women. J Appl Physiol,95,1833-1842.
  • 21. Olıveıra-Chıld, M., Leggate, M., Gleeson, M.(2013). Effects of Two Weeks of High-intensity Interval Training (HIIT) on Monocyte TLR2 and TLR4 Expression in High BMI Sedentary Men. International Journal of Exercise Science, 6 , 81-90.
  • 22. Radom-Aizik, S., Zaldivar, F., Stacy Oliver, S., Galassetti, P., et al.(2010. Evidence for microRNA involvement in exercise-associated neutrophil gene expression changes. J Appl Physiol ,109, 252–261

Yıl 2020, Cilt: 3 Sayı: 1, 40 - 46, 26.06.2020

Neşe Akpınar Kocakulak Serpi Taheri Elif Funda Şener Kenan Aycan Yusuf Özkul

https://doi.org/10.38061/idunas.726846

Öz

Proje Numarası

TSD-12-3929

Kaynakça

  • 1. Ntanasis, S, J., Tzanninis, J.G., Philippou, A.A., et al. (2013). Epigenetic regulation on gene expression induced by physical exercise. JMusculoskelet Neuronal Interact, 13, 133-146.
  • 2. Meier P, Renga M, Hoppeler H and Baum O . (2013).The impact of antioxidant supplements and endurance exercise on genes of the carbohydrate and lipid metabolism in skeletal muscle of mice. Cell Biochem Funct, 31, 51–59.
  • 3. Bangi, G., Delfabbro, M,. Maurş, C., Corsi, M., Melegati, G. (2000). Haematological parameters in elite rugby players during a competitive season. Clin.Lab.Haem, 28,183-188.
  • 4. Eynon, N., Meckel, Y., Alves, A.J., Yamin, C., Sagiv, M., et al.(2009). Is there an interaction between PPARD T294C and PPARGC1A gly482 Ser polymorphisms and human endurance performance? Exp physiol, 11,1147-1152.
  • 5. De Nadal, E., Ammerer, G., Posas, F. (2011). Controlling gene expression in response to stress. Nat Rev Genet, 12, 833–845.
  • 6. Flynn, MG., McFarlin BK., Phillips, MD., Stewart,LK., Timmerman, KL.(2003). Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise-trained elderly women, J Appl Physiol, 95(5), 1833–1842.
  • 7. Kumar, H., Kawai, T., Akira,S. (2009) Toll-like receptors and innate immunity. Biochem Biophys Res Commun, 388(4),621-5.
  • 8. Lancaster, GI., Khan, Q., Drysdale, P., et al.(2005). The physiological regulation of toll-like receptor expression and function in humans. J Physiol, 563, 945-955,
  • 9.Kocakulak,NA.,Hamurcu,Z.,Donmez-Altuntas, H., Sungur, G., Koca,F., Çoksevim,B.(2020). Effects of Exercise Performed at High Altitude on the Chromosomal DNA Damage in Human Peripheral Lymphocytes. Hacettepe J. Biol. & Chem, 48 (1), 13-20
  • 10. Kocakulak,NA.(2019). The Effect of Interval Training Program on Nuclear Factor Erythroid-Derived 2-like 2 (NFE2L2/Nrf2) Gene Expression in Women. Hacettepe J. Biol. & Chem, 47 (4), 410-414
  • 11. Gleeson, M. (2007). Immune function in sport and exercise. J Appl Physiol, 103,693-699.
  • 12. Borrs, C., Sastre, J., Garcfa-Sala, D., et al.(2003). Mitochond riafrom females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radic Biol Med, 34,546-552.
  • 13.Nilsson, C., Aboud, S., Karlen, K., et al.(2008). Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immunospot testing of healthy Swedish and Tanzanian subjects. Clin Vaccine Immunol,15, 585–9.
  • 14. Bayram, F., Diri, H., Sener, EF., Dundar,M., Simsek,Y.(2016). Genetic expressions of thrombophilic factors in patients with Sheehan’s syndrome. Gynecol Endocrinol,32(11), 908–911
  • 15. Jemiolo, B., Trappe,S. (2004). Single muscle fiber gene expression in human skeletal muscle: validation of internal contro lwith exercise,Biochem. Biophys. Res. Commun, 320(3),1043–1050.
  • 16. Catoire, M., Mensink, M., Boekschoten, MV., et al.(2012) Pronounced effects of acute endurance exercise on gene expression in resting and exercising human skeletal muscle, PloS One,7 (11) , e51066.
  • 17. Ahmetova, II., Popova, DV., Missinaa, SS., et al.(2010). Association of Mitochondrial Transcription Factor (TFAM) Gene Polymorphism with Physical Performance in Athletes. Human Physiology, 36,229–233.
  • 18. Parise, G., Brose, AN., Tarnopolsky, MA. (2005). Resistance exercise training decreases oxidative damage to DNA and increases cytochrome oxidase activity in older adults. Exp Gerontol,40, 173-80.
  • 19. Moldoveanu, AI., Shephard, RJ., Shek, PN.(2000). Exercise elevates plasma levels but not gene expression of IL-1b, IL-6, and TNF-a in blood mononuclear cells. J Appl Physiolog, 89,1499-1504.
  • 20. Flynn, MG., McFarlin, BK., Phillips, MD., et al.(2003). Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise-trained elderly women. J Appl Physiol,95,1833-1842.
  • 21. Olıveıra-Chıld, M., Leggate, M., Gleeson, M.(2013). Effects of Two Weeks of High-intensity Interval Training (HIIT) on Monocyte TLR2 and TLR4 Expression in High BMI Sedentary Men. International Journal of Exercise Science, 6 , 81-90.
  • 22. Radom-Aizik, S., Zaldivar, F., Stacy Oliver, S., Galassetti, P., et al.(2010. Evidence for microRNA involvement in exercise-associated neutrophil gene expression changes. J Appl Physiol ,109, 252–261
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Makaleler
Yazarlar

Neşe Akpınar Kocakulak 0000-0001-5798-263X

Serpi Taheri 0000-0001-5798-263X

Elif Funda Şener Bu kişi benim 0000-0001-5798-263X

Kenan Aycan 0000-0001-5798-263X

Yusuf Özkul 0000-0001-5798-263X

Proje Numarası TSD-12-3929
Yayımlanma Tarihi 26 Haziran 2020
Kabul Tarihi 25 Haziran 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 3 Sayı: 1

Kaynak Göster

APA Akpınar Kocakulak, N., Taheri, S., Şener, E. F., Aycan, K., vd. (2020). The Effect Of Different Workout Programs On The Expression Of The Genes Related To Oxidative Stress And Immune System. Natural and Applied Sciences Journal, 3(1), 40-46. https://doi.org/10.38061/idunas.726846
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