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The Protective Effect Mechanisms of Polyenylphosphatidylcholine PPC Pretreatment Against Stress Induced Ulcer in Rats

Yıl 2018, Cilt 7, Sayı 1, 1 - 9, 01.01.2018
https://doi.org/10.5505/abantmedj.2018.82150

Öz

INTRODUCTION: This study aims to investigate the mechanisms that are involved in protective actions exerted by pretreatment with polyenylphosphatidylcholine PPC against experimentally induced stress ulcer in rats.METHODS: Forty Swiss albino rats were divided into three groups. Group 1 n=10 was control, group 2 n=15 was stress ulcer and group 3 n=15 was PPC treated rats with stress ulcer. PPC treatment was started ten days before stress at a dose of 100 mg/day by oral route. Rats were terminated, stomachs were excised. Macroscopic ulcer index UI , production of reactive oxygen species, lipid peroxidation, plasma PGE2 and LTC4 levels, fatty-acid compositions were studied. The specimens were examined histopathologically.RESULTS: UI was significantly lower in the treatment group compared with non-treatment group. PGE2 decreased p=0.00 during cold restraint stress. PPC pretreatment was observed to inhibit the decrease in PGE2 in the induced stress ulcer. In the PPC treatment group LTC4 levels were significantly lower than non-treatment group p=0.00 . Additionally, PPC pretreatment inhibited degranülation of the submucosal mast cells. The inhibitory effect of PPC was more potent on red- stained mast cells than on blue-stained cells.DISCUSSION AND CONCLUSION: The present study shows that protective effects of PPC could be ascribed to inhibition of mast cell degranulation, a reduction in gastric oxidative injury, increase in biosynthesis of protective PGE2, and a decrease in biosynthesis of LTC4.

Kaynakça

  • Haglund U. Stress ulcers. Scand J Gastroenterol Suppl.1990; 175: 27-33.
  • Fennerty MB. Pathophysiology of the upper gastrointestinal tract in the critically ill patient: rationale for the therapeutic benefits of acid suppression. Crit Care Med. Jun; 2002; 30: 351-5.
  • Stein TA, Keegan LM, Auguste LJ, et al: Stress-induced gastric lesions and the synthesis of prostoglandis, and leukotriens. J Surg Res 1991; 51: 368-371.
  • Kwiecien S, Brzozowski T, Konturek SJ: Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury. J Physiol Pharmacol. 2002; 53: 39- 50
  • Kwiecień S, Brzozowski T, Konturek PCh, Konturek SJ: The role of reactive oxygen species in action of nitric oxide- donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol . 2002;53:761-73.
  • Demirbilek et al. Protective Effect of Polyunsatured Phosphatidylcholine Pretreatment on Stres Formation in Rats. Journal of Pedatric Surgery, 2004; 39: 57-62.
  • Das D, Banerjee RK: Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem 1993;125:1115-1125.
  • Tuncel N, Tuncel M,. Aboul-Enein HY: Effects of the vasoactive intestinal peptide on stress-induced mucosal ulcers and modulation of methylation of histamine in gastric tissue of the rats. Il Farmaco 2003; 58: 449-454.
  • Cho CH, Ogle CW: Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1- and H2- receptor activation in stress ulceration in rats. Eur J Pharmacol 1979; 55 : 23-33.
  • Uchiyoma M, Mihara M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 36: 271-278.
  • Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1975; 59: 245–249.
  • Green LC, Wagner DA, Glogowski J, et al: Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 126: 131-138,1982
  • Aebi H: Catalase. In: Methods of Enzymatic Analysis. Academic Press: New York: 1974; 673–67.
  • Beutler, E: Glutathione in red blood cell metabolism. A Manuel of Biochemical Methods, 1975.
  • Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin. Chem. 1988; 34: 497–500.
  • Goswami S, Mai J, Bruckner G, Kinsella J.E. Extraction and purification of prostaglandins and thromboxane from biological samples for gas chromatographic analysis. Prostaglandins 1981; 225:693-702.
  • Hara A, Radin NS. Lipid extraction of tissues with a low- toxicity solvent. Anal Biochem. 1978; 90: 420-6.
  • Tanaka R, Fujisawa S, Kawamura K, Harada M. Paraquat- induced enhancement of vascular permeability. J Toxicol Sci.1983; 8:147-59.
  • Crivellato E, Finato N, Isola M, Ribatti D, Beltrami CA: Low mast cell density in the human duodenal mucosa from chronic inflammatory duodenal bowel disorders is associated with defective villous architecture. Eur J Clin Invest.2003; 33:601-10.
  • Stassen M, Hultner L, Schmitt E: Classical and alternative pathways of mast cell activation. Crit Rev Immunol. 2002; 22:115-40.
  • Khadzhiev OC, Lupal'tsov VI, Simonenkov AP, Klimenko NA, Tatarko SV: Microcirculatory disturbances in gastric mucosa during ulcer disease and effects of serotonin on their dynamics. Bull Exp Biol Med.2000 ;130: 843-5.
  • Nishida K, Ohta Y, Kobayashi T, Ishiguro I: Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress. Digestion. 1997;58: 340-51.
  • Aleynik SI, Leo MA, Takeshige U, Aleynik MK, Lieber CS. Dilinoleoylphosphatidylcholine is the active antioxidant of polyenylphosphatidylcholine. Investig Med. 1999; 47: 507- 12.
  • Takeuchi K, Suzuki K, Araki H, Mizoguchi H, Sugamoto S, Umdeda M. Roles of endogenous prostaglandins and nitric oxide in gastroduodenal ulcerogenic responses induced in rats by hypothermic stress. J Physiol Paris. 1999; 93: 423- 31.
  • Brzozowski T, Konturek PC, Konturek SJ, Drozdowicz D, Pajdo R, Pawlik M, Brzozowska I, Hahn EG. Expression of cyclooxygenase (COX)-1 and COX-2 in adaptive cytoprotection induced by mild stress. J Physiol Paris. 2000; 94: 83-91.

Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları

Yıl 2018, Cilt 7, Sayı 1, 1 - 9, 01.01.2018
https://doi.org/10.5505/abantmedj.2018.82150

Öz

GİRİŞ ve AMAÇ: PPC ile prolifilaksinin, stres ülser gelişimini engellemede etkin olduğu daha önce yapılan deneysel bir çalışmada gösterilmiştir. Yine rat stres ülser modelinde gerçekleştirilen bu deneysel çalışmada, bu etkinliğin altında yatan mekanizmaların araştırılması amaçlanmıştır.YÖNTEM ve GEREÇLER: Ratlar, Grup1 n=10 kontrol, Grup2 n=15 stres ülser ve Grup3 n=15 PFK tedavisi + stres ülser olmak üzere üç gruba ayrıldı. Stres ülser modeli için Grup 2 ve grup 3 ratlar, 72 saatlik açlık periyodundan sonra, immobilize halde, +4 C?de, 4 saat soğuğa maruz bırakıldı. Grup 3 ratlara, PFK 100 mg/gün dozunda, oral yoldan, deney öncesi 10 gün süreyle verildi. Deney periyodu sonunda total çıkartılan ve büyük kurvatur boyunca açılan midelerde okülometrik yöntemle ülser indeksi UI hesaplandı Gastrik doku örneklerinde malondialdehit MDA ve superoksit dismutaz SOD , glutatyon GSH , ksantin oksidaz XO , eritrositlerde katalaz CAT , plazma örneklerinde ise total nitrit+nitrat, prostaglandin E2 PGE2 ve lökotrien C4 LTC4 düzeyleri ölçüldü. Histopatolojik incelemede gastrik mukozal hasar H&E boyama ve mast hücre degranülasyonu dominici boyama değerlendirildi.BULGULAR: Ülser indeksi UI ' nin PFK ile profilaksi grubunda, grup 2'den anlamlı düzeyde düşük olduğu görüldü p

Kaynakça

  • Haglund U. Stress ulcers. Scand J Gastroenterol Suppl.1990; 175: 27-33.
  • Fennerty MB. Pathophysiology of the upper gastrointestinal tract in the critically ill patient: rationale for the therapeutic benefits of acid suppression. Crit Care Med. Jun; 2002; 30: 351-5.
  • Stein TA, Keegan LM, Auguste LJ, et al: Stress-induced gastric lesions and the synthesis of prostoglandis, and leukotriens. J Surg Res 1991; 51: 368-371.
  • Kwiecien S, Brzozowski T, Konturek SJ: Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury. J Physiol Pharmacol. 2002; 53: 39- 50
  • Kwiecień S, Brzozowski T, Konturek PCh, Konturek SJ: The role of reactive oxygen species in action of nitric oxide- donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol . 2002;53:761-73.
  • Demirbilek et al. Protective Effect of Polyunsatured Phosphatidylcholine Pretreatment on Stres Formation in Rats. Journal of Pedatric Surgery, 2004; 39: 57-62.
  • Das D, Banerjee RK: Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem 1993;125:1115-1125.
  • Tuncel N, Tuncel M,. Aboul-Enein HY: Effects of the vasoactive intestinal peptide on stress-induced mucosal ulcers and modulation of methylation of histamine in gastric tissue of the rats. Il Farmaco 2003; 58: 449-454.
  • Cho CH, Ogle CW: Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1- and H2- receptor activation in stress ulceration in rats. Eur J Pharmacol 1979; 55 : 23-33.
  • Uchiyoma M, Mihara M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 36: 271-278.
  • Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1975; 59: 245–249.
  • Green LC, Wagner DA, Glogowski J, et al: Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 126: 131-138,1982
  • Aebi H: Catalase. In: Methods of Enzymatic Analysis. Academic Press: New York: 1974; 673–67.
  • Beutler, E: Glutathione in red blood cell metabolism. A Manuel of Biochemical Methods, 1975.
  • Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin. Chem. 1988; 34: 497–500.
  • Goswami S, Mai J, Bruckner G, Kinsella J.E. Extraction and purification of prostaglandins and thromboxane from biological samples for gas chromatographic analysis. Prostaglandins 1981; 225:693-702.
  • Hara A, Radin NS. Lipid extraction of tissues with a low- toxicity solvent. Anal Biochem. 1978; 90: 420-6.
  • Tanaka R, Fujisawa S, Kawamura K, Harada M. Paraquat- induced enhancement of vascular permeability. J Toxicol Sci.1983; 8:147-59.
  • Crivellato E, Finato N, Isola M, Ribatti D, Beltrami CA: Low mast cell density in the human duodenal mucosa from chronic inflammatory duodenal bowel disorders is associated with defective villous architecture. Eur J Clin Invest.2003; 33:601-10.
  • Stassen M, Hultner L, Schmitt E: Classical and alternative pathways of mast cell activation. Crit Rev Immunol. 2002; 22:115-40.
  • Khadzhiev OC, Lupal'tsov VI, Simonenkov AP, Klimenko NA, Tatarko SV: Microcirculatory disturbances in gastric mucosa during ulcer disease and effects of serotonin on their dynamics. Bull Exp Biol Med.2000 ;130: 843-5.
  • Nishida K, Ohta Y, Kobayashi T, Ishiguro I: Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress. Digestion. 1997;58: 340-51.
  • Aleynik SI, Leo MA, Takeshige U, Aleynik MK, Lieber CS. Dilinoleoylphosphatidylcholine is the active antioxidant of polyenylphosphatidylcholine. Investig Med. 1999; 47: 507- 12.
  • Takeuchi K, Suzuki K, Araki H, Mizoguchi H, Sugamoto S, Umdeda M. Roles of endogenous prostaglandins and nitric oxide in gastroduodenal ulcerogenic responses induced in rats by hypothermic stress. J Physiol Paris. 1999; 93: 423- 31.
  • Brzozowski T, Konturek PC, Konturek SJ, Drozdowicz D, Pajdo R, Pawlik M, Brzozowska I, Hahn EG. Expression of cyclooxygenase (COX)-1 and COX-2 in adaptive cytoprotection induced by mild stress. J Physiol Paris. 2000; 94: 83-91.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Research Article
Yazarlar

Necla GÜRBÜZ SARIKAŞ Bu kişi benim
Sağlık Bilimleri Üniveritesi, Derince Eğitim ve Araştırma Hastanesi, Çocuk Cerrahisi Kliniği, Kocaeli, Türkiye


Melih AKIN Bu kişi benim
Sağlık Bilimleri Üniveritesi, İstanbul Şişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi, Çocuk Cerrahisi Kliniği, İstanbul, Türkiye


Kubilay GÜRÜNLÜOĞLU Bu kişi benim
İnönü Üniveritesi Tıp Fakültesi, Çocuk Cerrahisi Bölümü, Malatya, Türkiye


Erkan TAŞ Bu kişi benim
Özel Batman Dünya Hastanesi, Çocuk Cerrahisi Bölümü, Batman, Türkiye


Aysun Bay KARABULUT Bu kişi benim
Ankara Yıldırım Beyazıt Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, Ankara, Türkiye


İclal GÜRSES Bu kişi benim
Mersin Üniversitesi Tip Fakültesi, Patoloji Anabilim Dalı, Mersin, Türkiye

Yayımlanma Tarihi 1 Ocak 2018
Başvuru Tarihi
Kabul Tarihi
Yayınlandığı Sayı Yıl 2018, Cilt 7, Sayı 1

Kaynak Göster

Bibtex @ { abantmedj766378, journal = {Abant Tıp Dergisi}, issn = {1305-4392}, eissn = {2147-1800}, address = {Abant Tıp Dergisi Editörlüğü Bolu Abant İzzet Baysal Üniversitesi Tıp Fakültesi, 14280 / BOLU}, publisher = {Bolu Abant İzzet Baysal Üniversitesi}, year = {2018}, volume = {7}, number = {1}, pages = {1 - 9}, doi = {10.5505/abantmedj.2018.82150}, title = {Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları}, key = {cite}, author = {Gürbüz Sarıkaş, Necla and Akın, Melih and Gürünlüoğlu, Kubilay and Taş, Erkan and Karabulut, Aysun Bay and Gürses, İclal} }
APA Gürbüz Sarıkaş, N. , Akın, M. , Gürünlüoğlu, K. , Taş, E. , Karabulut, A. B. & Gürses, İ. (2018). Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları . Abant Tıp Dergisi , 7 (1) , 1-9 . DOI: 10.5505/abantmedj.2018.82150
MLA Gürbüz Sarıkaş, N. , Akın, M. , Gürünlüoğlu, K. , Taş, E. , Karabulut, A. B. , Gürses, İ. "Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları" . Abant Tıp Dergisi 7 (2018 ): 1-9 <https://dergipark.org.tr/tr/pub/abantmedj/issue/55915/766378>
Chicago Gürbüz Sarıkaş, N. , Akın, M. , Gürünlüoğlu, K. , Taş, E. , Karabulut, A. B. , Gürses, İ. "Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları". Abant Tıp Dergisi 7 (2018 ): 1-9
RIS TY - JOUR T1 - Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları AU - Necla Gürbüz Sarıkaş , Melih Akın , Kubilay Gürünlüoğlu , Erkan Taş , Aysun Bay Karabulut , İclal Gürses Y1 - 2018 PY - 2018 N1 - doi: 10.5505/abantmedj.2018.82150 DO - 10.5505/abantmedj.2018.82150 T2 - Abant Tıp Dergisi JF - Journal JO - JOR SP - 1 EP - 9 VL - 7 IS - 1 SN - 1305-4392-2147-1800 M3 - doi: 10.5505/abantmedj.2018.82150 UR - https://doi.org/10.5505/abantmedj.2018.82150 Y2 - 2022 ER -
EndNote %0 Abant Tıp Dergisi Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları %A Necla Gürbüz Sarıkaş , Melih Akın , Kubilay Gürünlüoğlu , Erkan Taş , Aysun Bay Karabulut , İclal Gürses %T Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları %D 2018 %J Abant Tıp Dergisi %P 1305-4392-2147-1800 %V 7 %N 1 %R doi: 10.5505/abantmedj.2018.82150 %U 10.5505/abantmedj.2018.82150
ISNAD Gürbüz Sarıkaş, Necla , Akın, Melih , Gürünlüoğlu, Kubilay , Taş, Erkan , Karabulut, Aysun Bay , Gürses, İclal . "Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları". Abant Tıp Dergisi 7 / 1 (Ocak 2018): 1-9 . https://doi.org/10.5505/abantmedj.2018.82150
AMA Gürbüz Sarıkaş N. , Akın M. , Gürünlüoğlu K. , Taş E. , Karabulut A. B. , Gürses İ. Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Med J. 2018; 7(1): 1-9.
Vancouver Gürbüz Sarıkaş N. , Akın M. , Gürünlüoğlu K. , Taş E. , Karabulut A. B. , Gürses İ. Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Tıp Dergisi. 2018; 7(1): 1-9.
IEEE N. Gürbüz Sarıkaş , M. Akın , K. Gürünlüoğlu , E. Taş , A. B. Karabulut ve İ. Gürses , "Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları", Abant Tıp Dergisi, c. 7, sayı. 1, ss. 1-9, Oca. 2018, doi:10.5505/abantmedj.2018.82150