The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells

Yıl 2021, Cilt: 10 Sayı: 2, 212 - 218, 31.08.2021

Özlem Özgür Gürsoy Canan Vejselova Sezer Ceren Yıldız Eren Hulusi Göktuğ Gürer

https://doi.org/10.47493/abantmedj.881050

Öz

INTRODUCTION: This study aims to exhibit the growth inhibitory effect of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of hyperthermia.
METHODS: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V and reactive oxygen species (ROS) measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia.
RESULTS: Quercetin has good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin.
DISCUSSION and CONCLUSION: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

Anahtar Kelimeler

Quercetin, OVCAR-3 cells, hyperthermia

Kuersetin ve Hiperterminin OVCAR-3 Hücrelerine Etkileri

Öz

Aim: the aim of this study is to exhibit the growth inhibitory effect of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of hyperthermia.
Materials and Methods: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V analysis and ROS measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia.
Results: Quercetin have good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin.
Conclusion: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

Anahtar Kelimeler

kuersetin, OVCAR-3 hücreleri, hipertermi

Kaynakça

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