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The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells

Yıl 2021, Cilt 10, Sayı 2, 212 - 218, 31.08.2021

Öz

INTRODUCTION: This study aims to exhibit the growth inhibitory effect of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of hyperthermia.
METHODS: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V and reactive oxygen species (ROS) measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia.
RESULTS: Quercetin has good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin.
DISCUSSION and CONCLUSION: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

Kaynakça

  • 1. Siegel RL, Miller KD, Jemal A. Cancer statistics, CA Cancer J Clin 2018; 68: 7-30.
  • 2. Yap TA, Carden CP, Kaye SB. Beyond chemotherapy: targeted therapies in ovarian cancer. Nat Rev Cancer 2009; 9: 167-81.
  • 3. Bakrin N, Classe JM, Pomel C, Gouy S, Chene G, Glehen O. Hyperthermic intraperitoneal chemotherapy (HIPEC) in ovarian cancer. J Visc Surg 2014; 151: 347-53.
  • 4. Ozols RF, Bundy BN, Greer BE, Fowler JM, Clarke Pearson D, Burger RA, Mannel RS, DeGeest K, Hartenbach EM, Baergen R. Gynecologic Oncology G: Phase III trial of carboplatin and paclitaxel compared with cisplatin and paclitaxel in patients with optimally resected stage III ovarian cancer: a Gynecologic Oncology Group study. J Clin Oncol 2003; 21: 3194-200.
  • 5. Surh YJ. Cancer chemoprevention with dietary phytochemicals.Nat Rev Cancer 2003; 3: 768-80.
  • 6. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer.Biochem Pharmacol 2006; 71: 1397-421.
  • 7. Batra, P., Sharma, A.K. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech 2013; 3: 439–59.
  • 8. Tanigawa, Shunsuke & Fujii, Makoto & Hou, De-Xing. Stabilization of p53 Is Involved in Quercetin-Induced Cell Cycle Arrest and Apoptosis in HepG2 Cells. Bioscience, biotechnology, and biochemistry 2008; 72: 797-804.
  • 9. Sukovas A, Cesna V, Jasukaitiene A, Barauskas G, Nadisauskiene RJ, Dambrauskas Z, Paskauskas S, Gulbinas A. Response of OVCAR-3 Cells to Cisplatin and Hyperthermia: Does Hyperthermia Really Matter? Anticancer Res 2017; 37: 5011-18.
  • 10. van de Vaart PJ, van der Vange N, Zoetmulder FA, van Goethem AR, van Tellingen O, ten Bokkel Huinink WW, Beijnen JH, Bartelink H, Begg AC: Intraperitoneal cisplatin with regional hyperthermia in advanced ovarian cancer: pharmacokinetics and cisplatin-DNA adduct formation in patients and ovarian cancer cell lines. Eur J Cancer 1998; 34: 148-54.
  • 11.Bakrin N, Cotte E, Golfier F, Gilly FN, Freyer G, Helm W, Glehen O, Bereder JM: Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (HIPEC) for persistent and recurrent advanced ovarian carcinoma: a multicenter, prospective study of 246 patients. Ann Surg Oncol 2012; 19: 4052-58.
  • 12. Hazafa, Abu & Rehman, Khalil-Ur & Jahan, Nazish & Jabeen, Zara. The Role of Polyphenol (Flavonoids) Compounds in the Treatment of Cancer Cells. Nutrition and Cancer 2019; 72: 1-12.
  • 13. Amidi, F., Rashidi, Z., Khosravizadeh, Z., Khodamoradi, K., Talebi, A.,Navid, S., & Abbasi, M. Antioxidant effects of quercetin in freeze-thawing process of mouse spermatogonial stem cells. Asian Pacific Journal of Reproduction 2019; 8: 7.
  • 14. Anand David AV, Arulmoli R, Parasuraman S. Overviews of Biological Importance of Quercetin: A Bioactive Flavonoid. Pharmacogn Rev 2016; 10: 84-9.
  • 15. Vafadar A, Shabaninejad Z, Movahedpour A. Quercetin and cancer: new insights into its therapeutic effects on ovarian cancer cells. Cell Biosci 2020; 10: 32.
  • 16. Myhrstad MC, Carlsen H, Nordström O, Blomhoff R, Moskaug JØ. Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter. Free Radic Biol Med 2002; 32: 386-93.
  • 17. Bądziul, D., Jakubowicz-Gil, J., Paduch, R. Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells. Mol Cell Biochem 2014; 392: 213–27.
  • 18. Kujawski R, Baraniak J, Ożarowski M, Kujawska M, Borowska M, Kania M. Basic Studies on Chemopreventive Properties of Quercetin and Curcumin and Other Plant-origin Compounds in Ovarian Cancer Cells – A Mini-review. Alternative & Integrative Medicine 2017; 6: 2.
  • 19. Maciejczyk, Adam & Surowiak, Pawel. Quercetin inhibits proliferation and increases sensitivity of ovarian cancer cells to cisplatin and paclitaxel. Ginekologia polska 2013;.84: 590-5.
  • 20. Parvaresh, A., Razavi, R., Rafie, N., Ghiasvand, R., Pourmasoumi, M., &Miraghajani, M. Quercetin and ovarian cancer: An evaluation based on a systematic review. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences 2016; 9: 34–40.
  • 21. Gao, X., Wang, B., Wei, X., Men, K., Zheng, F., Zhou, Y, Guo, G. Anticancer effect and mechanism of polymer micelle-encapsulated quercetin on ovarian cancer. Nanoscale 2012; 4: 7021–30.
  • 22. Wang, Y., Han, A., Chen, E., Singh, R. K., Chichester, C. O., Moore, R. G., Vorsa, N. The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells. International Journal of Oncology 2015; 46: 1924–34.
  • 23. Najafi, Masoud & Tavakol, Shima & Zarrabi, Ali & Ashrafizadeh, Milad. Dual role of quercetin in enhancing the efficacy of cisplatin in chemotherapy and protection against its side effects: a review. Archives of Physiology and Biochemistry 2020; 10: 1-15.
  • 24. Catanzaro, Daniela & Ragazzi, Eugenio & Vianello, Caterina & Caparrotta, Laura & Montopoli, Monica. Effect of Quercetin on Cell Cycle and Cyclin Expression in Ovarian Carcinoma and Osteosarcoma Cell Lines. Natural product communications 2015; 10: 1365-8.
  • 25. Petrillo M, Zucchetti M, Cianci S, Morosi L, Ronsini C, Colombo A, D'Incalci M, Scambia G, Fagotti A. Pharmacokinetics of cisplatin during open and minimally-invasive secondary cytoreductive surgery plus HIPEC in women with platinum-sensitive recurrent ovarian cancer: A prospective study. J. Gynecol. Oncol 2019; 30: e59.

Kuersetin ve Hiperterminin OVCAR-3 Hücrelerine Etkileri

Yıl 2021, Cilt 10, Sayı 2, 212 - 218, 31.08.2021

Öz

Aim: the aim of this study is to exhibit the growth inhibitory effect of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of hyperthermia. Materials and Methods: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V analysis and ROS measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia. Results: Quercetin have good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin. Conclusion: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

Kaynakça

  • 1. Siegel RL, Miller KD, Jemal A. Cancer statistics, CA Cancer J Clin 2018; 68: 7-30.
  • 2. Yap TA, Carden CP, Kaye SB. Beyond chemotherapy: targeted therapies in ovarian cancer. Nat Rev Cancer 2009; 9: 167-81.
  • 3. Bakrin N, Classe JM, Pomel C, Gouy S, Chene G, Glehen O. Hyperthermic intraperitoneal chemotherapy (HIPEC) in ovarian cancer. J Visc Surg 2014; 151: 347-53.
  • 4. Ozols RF, Bundy BN, Greer BE, Fowler JM, Clarke Pearson D, Burger RA, Mannel RS, DeGeest K, Hartenbach EM, Baergen R. Gynecologic Oncology G: Phase III trial of carboplatin and paclitaxel compared with cisplatin and paclitaxel in patients with optimally resected stage III ovarian cancer: a Gynecologic Oncology Group study. J Clin Oncol 2003; 21: 3194-200.
  • 5. Surh YJ. Cancer chemoprevention with dietary phytochemicals.Nat Rev Cancer 2003; 3: 768-80.
  • 6. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer.Biochem Pharmacol 2006; 71: 1397-421.
  • 7. Batra, P., Sharma, A.K. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech 2013; 3: 439–59.
  • 8. Tanigawa, Shunsuke & Fujii, Makoto & Hou, De-Xing. Stabilization of p53 Is Involved in Quercetin-Induced Cell Cycle Arrest and Apoptosis in HepG2 Cells. Bioscience, biotechnology, and biochemistry 2008; 72: 797-804.
  • 9. Sukovas A, Cesna V, Jasukaitiene A, Barauskas G, Nadisauskiene RJ, Dambrauskas Z, Paskauskas S, Gulbinas A. Response of OVCAR-3 Cells to Cisplatin and Hyperthermia: Does Hyperthermia Really Matter? Anticancer Res 2017; 37: 5011-18.
  • 10. van de Vaart PJ, van der Vange N, Zoetmulder FA, van Goethem AR, van Tellingen O, ten Bokkel Huinink WW, Beijnen JH, Bartelink H, Begg AC: Intraperitoneal cisplatin with regional hyperthermia in advanced ovarian cancer: pharmacokinetics and cisplatin-DNA adduct formation in patients and ovarian cancer cell lines. Eur J Cancer 1998; 34: 148-54.
  • 11.Bakrin N, Cotte E, Golfier F, Gilly FN, Freyer G, Helm W, Glehen O, Bereder JM: Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (HIPEC) for persistent and recurrent advanced ovarian carcinoma: a multicenter, prospective study of 246 patients. Ann Surg Oncol 2012; 19: 4052-58.
  • 12. Hazafa, Abu & Rehman, Khalil-Ur & Jahan, Nazish & Jabeen, Zara. The Role of Polyphenol (Flavonoids) Compounds in the Treatment of Cancer Cells. Nutrition and Cancer 2019; 72: 1-12.
  • 13. Amidi, F., Rashidi, Z., Khosravizadeh, Z., Khodamoradi, K., Talebi, A.,Navid, S., & Abbasi, M. Antioxidant effects of quercetin in freeze-thawing process of mouse spermatogonial stem cells. Asian Pacific Journal of Reproduction 2019; 8: 7.
  • 14. Anand David AV, Arulmoli R, Parasuraman S. Overviews of Biological Importance of Quercetin: A Bioactive Flavonoid. Pharmacogn Rev 2016; 10: 84-9.
  • 15. Vafadar A, Shabaninejad Z, Movahedpour A. Quercetin and cancer: new insights into its therapeutic effects on ovarian cancer cells. Cell Biosci 2020; 10: 32.
  • 16. Myhrstad MC, Carlsen H, Nordström O, Blomhoff R, Moskaug JØ. Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter. Free Radic Biol Med 2002; 32: 386-93.
  • 17. Bądziul, D., Jakubowicz-Gil, J., Paduch, R. Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells. Mol Cell Biochem 2014; 392: 213–27.
  • 18. Kujawski R, Baraniak J, Ożarowski M, Kujawska M, Borowska M, Kania M. Basic Studies on Chemopreventive Properties of Quercetin and Curcumin and Other Plant-origin Compounds in Ovarian Cancer Cells – A Mini-review. Alternative & Integrative Medicine 2017; 6: 2.
  • 19. Maciejczyk, Adam & Surowiak, Pawel. Quercetin inhibits proliferation and increases sensitivity of ovarian cancer cells to cisplatin and paclitaxel. Ginekologia polska 2013;.84: 590-5.
  • 20. Parvaresh, A., Razavi, R., Rafie, N., Ghiasvand, R., Pourmasoumi, M., &Miraghajani, M. Quercetin and ovarian cancer: An evaluation based on a systematic review. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences 2016; 9: 34–40.
  • 21. Gao, X., Wang, B., Wei, X., Men, K., Zheng, F., Zhou, Y, Guo, G. Anticancer effect and mechanism of polymer micelle-encapsulated quercetin on ovarian cancer. Nanoscale 2012; 4: 7021–30.
  • 22. Wang, Y., Han, A., Chen, E., Singh, R. K., Chichester, C. O., Moore, R. G., Vorsa, N. The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells. International Journal of Oncology 2015; 46: 1924–34.
  • 23. Najafi, Masoud & Tavakol, Shima & Zarrabi, Ali & Ashrafizadeh, Milad. Dual role of quercetin in enhancing the efficacy of cisplatin in chemotherapy and protection against its side effects: a review. Archives of Physiology and Biochemistry 2020; 10: 1-15.
  • 24. Catanzaro, Daniela & Ragazzi, Eugenio & Vianello, Caterina & Caparrotta, Laura & Montopoli, Monica. Effect of Quercetin on Cell Cycle and Cyclin Expression in Ovarian Carcinoma and Osteosarcoma Cell Lines. Natural product communications 2015; 10: 1365-8.
  • 25. Petrillo M, Zucchetti M, Cianci S, Morosi L, Ronsini C, Colombo A, D'Incalci M, Scambia G, Fagotti A. Pharmacokinetics of cisplatin during open and minimally-invasive secondary cytoreductive surgery plus HIPEC in women with platinum-sensitive recurrent ovarian cancer: A prospective study. J. Gynecol. Oncol 2019; 30: e59.

Ayrıntılar

Birincil Dil İngilizce
Konular Tıp
Bölüm Araştırma Makaleleri
Yazarlar

Özlem ÖZGÜR GÜRSOY (Sorumlu Yazar)
Eskisehir Acibadem Hospital
0000-0001-2345-6789
Türkiye


Canan VEJSELOVA SEZER
FEN FAKÜLTESİ
0000-0002-3792-5993
Türkiye


Ceren YILDIZ EREN
Eskişehir Acibadem Hospital
0000-0003-1648-3345
Türkiye


Hulusi Göktuğ GÜRER
Eskisehir Acibadem Hospital
0000-0001-8821-9469
Türkiye

Yayımlanma Tarihi 31 Ağustos 2021
Başvuru Tarihi 16 Şubat 2021
Kabul Tarihi 3 Haziran 2021
Yayınlandığı Sayı Yıl 2021, Cilt 10, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { abantmedj881050, journal = {Abant Tıp Dergisi}, issn = {1305-4392}, eissn = {2147-1800}, address = {Abant Tıp Dergisi Editörlüğü Bolu Abant İzzet Baysal Üniversitesi Tıp Fakültesi, 14280 / BOLU}, publisher = {Abant İzzet Baysal Üniversitesi}, year = {2021}, volume = {10}, pages = {212 - 218}, doi = {10.47493/abantmedj.881050}, title = {The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells}, key = {cite}, author = {Özgür Gürsoy, Özlem and Vejselova Sezer, Canan and Yıldız Eren, Ceren and Gürer, Hulusi Göktuğ} }
APA Özgür Gürsoy, Ö. , Vejselova Sezer, C. , Yıldız Eren, C. & Gürer, H. G. (2021). The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells . Abant Tıp Dergisi , 10 (2) , 212-218 . Retrieved from https://dergipark.org.tr/tr/pub/abantmedj/issue/64672/881050
MLA Özgür Gürsoy, Ö. , Vejselova Sezer, C. , Yıldız Eren, C. , Gürer, H. G. "The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells" . Abant Tıp Dergisi 10 (2021 ): 212-218 <https://dergipark.org.tr/tr/pub/abantmedj/issue/64672/881050>
Chicago Özgür Gürsoy, Ö. , Vejselova Sezer, C. , Yıldız Eren, C. , Gürer, H. G. "The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells". Abant Tıp Dergisi 10 (2021 ): 212-218
RIS TY - JOUR T1 - The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells AU - Özlem Özgür Gürsoy , Canan Vejselova Sezer , Ceren Yıldız Eren , Hulusi Göktuğ Gürer Y1 - 2021 PY - 2021 N1 - DO - T2 - Abant Tıp Dergisi JF - Journal JO - JOR SP - 212 EP - 218 VL - 10 IS - 2 SN - 1305-4392-2147-1800 M3 - UR - Y2 - 2021 ER -
EndNote %0 Abant Tıp Dergisi The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells %A Özlem Özgür Gürsoy , Canan Vejselova Sezer , Ceren Yıldız Eren , Hulusi Göktuğ Gürer %T The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells %D 2021 %J Abant Tıp Dergisi %P 1305-4392-2147-1800 %V 10 %N 2 %R %U
ISNAD Özgür Gürsoy, Özlem , Vejselova Sezer, Canan , Yıldız Eren, Ceren , Gürer, Hulusi Göktuğ . "The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells". Abant Tıp Dergisi 10 / 2 (Ağustos 2021): 212-218 .
AMA Özgür Gürsoy Ö. , Vejselova Sezer C. , Yıldız Eren C. , Gürer H. G. The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells. Abant Tıp Derg. 2021; 10(2): 212-218.
Vancouver Özgür Gürsoy Ö. , Vejselova Sezer C. , Yıldız Eren C. , Gürer H. G. The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells. Abant Tıp Dergisi. 2021; 10(2): 212-218.
IEEE Ö. Özgür Gürsoy , C. Vejselova Sezer , C. Yıldız Eren ve H. G. Gürer , "The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells", Abant Tıp Dergisi, c. 10, sayı. 2, ss. 212-218, Ağu. 2021, doi:10.47493/abantmedj.881050