Influence of Enterococcus faecium, a probiotic component, on ion channels in colon cancer

Abstract

Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium (E. faecium) on the gene expression of ion channels in colon cancer cells. Methods: The cytotoxic effect on SW480 colon cancer cell line was analyzed by MTT cell proliferation test using E. faecium bacterial cell culture free supernatants. The effect of E. faecium on ion channel genes in SW480 cells was determined by qRT PCR analysis. STRING analysis was used to reveal protein-protein interactions of ion channel proteins. Bioinformatic analysis of healthy and colon cancer patient data on ion channels was revealed with GEPIA platform. Results: The SW480 cell line's viability was enhanced by E. fecieum bacterial cell culture free supernatants dosages of 0.5 and 1.09 mg/ml, but it dramatically declined between 2.187 and 17.5 mg/ml doses, according the MTT study. Analysis of gene expression revealed that the TRPV2 and TRPM8 genes had significantly increased. The examined ion channel proteins were discovered to be substantially linked to one another based on STRING analysis. The TRPM2 gene we looked at revealed a notable rise in colon cancer patients based on data from both healthy and colon cancer patients on the GEPIA platform. Conclusion: E. faecium has been shown to have many beneficial effects on health. Our study has shown that it also has an effect on ion channels in cancer cells and that ion channels are of great importance for cell survival and death.

Keywords

• Colon cancer
• ion channels
• microbiota
• E. faecium

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