Yüksek fruktoz tüketiminin dişi Sprague Dawley ratlarda metabolik parametreler ve karaciğer histolojisi üzerine etkileri

Abstract

Giriş/Amaç: Bu çalışmada, uzun süreli yüksek fruktoz tüketiminin dişi Sprague Dawley ratlarda metabolik parametreler ve karaciğer histolojisi üzerindeki etkileri araştırılmıştır. Materyal ve Metot: Deney grubuna 90 gün boyunca %20 fruktoz takviyeli su verilirken, kontrol grubu standart diyetle beslenmiştir. Bulgular/Sonuçlar: Sonuçlara göre, fruktoz grubunda yem tüketimi (356.47 ± 83.40 g vs. 1078.67 ± 79.97 g) ve protein alımı (81.28 ± 19.02 g vs. 245.94 ± 18.23 g) anlamlı derecede azalmış (p<0.001), su tüketimi ise belirgin şekilde artmıştır (2612.56 ± 474.13 mL vs. 1662.03 ± 121.70 mL; p<0.001). Enerji alımı (2898.43 ± 526.65 kcal vs. 3052.63 ± 226.32 kcal) ve vücut ağırlığı (1541.97 ± 287.67 g vs. 1575.97 ± 63.24 g) açısından gruplar arasında istatistiksel fark bulunmamıştır (p>0.05). Histopatolojik incelemelerde, deney grubunda hepatositlerin %33'ünden azını etkileyen 1. derece makroveziküler steatoz tespit edilirken, kontrol grubunda patolojik bulguya rastlanmamıştır. Sonuç: Bulgular, fruktozun de novo lipogenez yoluyla karaciğerde yağ birikimini tetiklediğini, ancak enerji dengesini koruyarak vücut ağırlığını etkilemediğini göstermektedir. Çalışma, yüksek fruktoz alımının hayvan modellerinde hepatik steatoz ve metabolik adaptasyonlara yol açtığını ortaya koymuştur. Anahtar Kelimeler: fruktoz, karaciğer yağlanması, sprague dawley rat, metabolik parametreler

Keywords

• fruktoz
• karaciğer yağlanması
• sprague dawley rat
• metabolik parametreler

Effects of high fructose consumption on metabolic parameters and liver histology in female sprague dawley rats

Abstract

Aims: This study investigated the effects of long-term high fructose consumption on metabolic parameters and liver histology in female Sprague Dawley rats. Methods: The experimental group received 20% fructose-supplemented water for 90 days, while the control group was fed a standard diet. Results: Results showed that the fructose group had significantly lower feed intake (356.47±83.40 g vs. 1078.67±79.97 g) and protein intake (81.28±19.02 g vs. 245.94±18.23 g; p<0.001), but higher water consumption (2612.56±474.13 ml vs. 1662.03±121.70 ml; p<0.001). No statistical differences were observed in energy intake (2898.43±526.65 kcal vs. 3052.63±226.32 kcal) or body weight (1541.97±287.67 g vs. 1575.97±63.24 g; p>0.05). Histopathological analysis revealed grade 1 macrovesicular steatosis (affecting <33% of hepatocytes) in the fructose group, while the control group showed no pathological changes. Conclusion: The findings suggest that fructose triggers hepatic fat accumulation via de novo lipogenesis without disrupting energy balance or body weight. This study demonstrates that high fructose intake induces hepatic steatosis and metabolic adaptations in animal models, highlighting its potential role in diet-related metabolic disorders.

Keywords

• Fructose
• hepatic steatosis
• sprague dawley rat
• metabolic parameters

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