Yüksek fruktoz tüketiminin dişi Sprague Dawley ratlarda metabolik parametreler ve karaciğer histolojisi üzerine etkileri

Year 2026, Volume: 8 Issue: 1, 97 - 102, 06.01.2026

Can Ayhan Kaya , İbrahim Hanifi Özercan

https://doi.org/10.38053/acmj.1801083

Abstract

Giriş/Amaç: Bu çalışmada, uzun süreli yüksek fruktoz tüketiminin dişi Sprague Dawley ratlarda metabolik parametreler ve karaciğer histolojisi üzerindeki etkileri araştırılmıştır. Materyal ve Metot: Deney grubuna 90 gün boyunca %20 fruktoz takviyeli su verilirken, kontrol grubu standart diyetle beslenmiştir. Bulgular/Sonuçlar: Sonuçlara göre, fruktoz grubunda yem tüketimi (356.47 ± 83.40 g vs. 1078.67 ± 79.97 g) ve protein alımı (81.28 ± 19.02 g vs. 245.94 ± 18.23 g) anlamlı derecede azalmış (p<0.001), su tüketimi ise belirgin şekilde artmıştır (2612.56 ± 474.13 mL vs. 1662.03 ± 121.70 mL; p<0.001). Enerji alımı (2898.43 ± 526.65 kcal vs. 3052.63 ± 226.32 kcal) ve vücut ağırlığı (1541.97 ± 287.67 g vs. 1575.97 ± 63.24 g) açısından gruplar arasında istatistiksel fark bulunmamıştır (p>0.05). Histopatolojik incelemelerde, deney grubunda hepatositlerin %33'ünden azını etkileyen 1. derece makroveziküler steatoz tespit edilirken, kontrol grubunda patolojik bulguya rastlanmamıştır. Sonuç: Bulgular, fruktozun de novo lipogenez yoluyla karaciğerde yağ birikimini tetiklediğini, ancak enerji dengesini koruyarak vücut ağırlığını etkilemediğini göstermektedir. Çalışma, yüksek fruktoz alımının hayvan modellerinde hepatik steatoz ve metabolik adaptasyonlara yol açtığını ortaya koymuştur.
Anahtar Kelimeler: fruktoz, karaciğer yağlanması, sprague dawley rat, metabolik parametreler

Keywords

fruktoz , karaciğer yağlanması , sprague dawley rat , metabolik parametreler

Ethical Statement

Protokol No: 12.08.2025-37784

Effects of high fructose consumption on metabolic parameters and liver histology in female sprague dawley rats

Abstract

Aims: This study investigated the effects of long-term high fructose consumption on metabolic parameters and liver histology in female Sprague Dawley rats.
Methods: The experimental group received 20% fructose-supplemented water for 90 days, while the control group was fed a standard diet.
Results: Results showed that the fructose group had significantly lower feed intake (356.47±83.40 g vs. 1078.67±79.97 g) and protein intake (81.28±19.02 g vs. 245.94±18.23 g; p<0.001), but higher water consumption (2612.56±474.13 ml vs. 1662.03±121.70 ml; p<0.001). No statistical differences were observed in energy intake (2898.43±526.65 kcal vs. 3052.63±226.32 kcal) or body weight (1541.97±287.67 g vs. 1575.97±63.24 g; p>0.05). Histopathological analysis revealed grade 1 macrovesicular steatosis (affecting <33% of hepatocytes) in the fructose group, while the control group showed no pathological changes.
Conclusion: The findings suggest that fructose triggers hepatic fat accumulation via de novo lipogenesis without disrupting energy balance or body weight. This study demonstrates that high fructose intake induces hepatic steatosis and metabolic adaptations in animal models, highlighting its potential role in diet-related metabolic disorders.

Keywords

Fructose , hepatic steatosis , sprague dawley rat , metabolic parameters

Ethical Statement

Approved Protocol No: 12.08.2025-37784

References

• Tappy L, Le KA. Metabolic effects of fructose and the worldwide increase in obesity. Physiological Reviews. 2010;90(1):23-46. doi:10.1152/physrev.00019.2009
• Stanhope KL, Havel PJ. Fructose consumption: recent results and their potential implications. Ann N Y Acad Sci. 2010;1190:15-24. doi:10.1111/j. 1749-6632.2009.05266.x
• Vos MB, Lavine JE. Dietary fructose in nonalcoholic fatty liver disease. Hepatology. 2013;57(6):2525–2531. doi:10.1002/hep.26299
• Abdelmalek MF, Suzuki A, Guy C, et al. Increased fructose consumption is associated with fibrosis severity in patients with NAFLD. Hepatology. 2010;51(6):1961-1971. doi:10.1002/hep.23535
• Spruss A, Bergheim I. Dietary fructose and intestinal barrier: potential risk factor in the pathogenesis of nonalcoholic fatty liver disease. J Nutr Biochem. 2009;20:657-662. doi:10.1016/j.jnutbio.2009.05.006
• Ramos V, Batista LO, Albuquerque KT. Effects of fructose consumption on food intake and biochemical and body parameters in Wistar rats. Rev Port Cardiol. 2017. doi:10.1016/J.REPC.2017.04.003
• Chan AML, Ng AMH, Mohd Yunus MH, et al. Recent developments in rodent models of high-fructose diet-ınduced metabolic syndrome: a systematic review. Nutrients. 2021;13(8):2497. doi:10.3390/nu13082497
• Herdt TH. Ruminant adaptation to negative energy balance: influences on the etiology of ketosis and fatty liver. Vet Clin North Am Food Anim Pract. 2000;16(2):215-230. doi:10.1016/S0749-0720(15)30102-X
• Goff JP, Horst RL. Physiological changes at parturition and their relationship to metabolic disorders. J Dairy Sci. 1997;80(7):1260–1268. doi:10.3168/jds.S0022-0302(97)76055-7
• King S, Baptiston Tanaka C, Ross D, et al. A diet high in fat and fructose adversely affects osseointegration of titanium implants in rats. Clin Exp Dent Res. 2020;6(1):107-116. doi:10.1002/cre2.255
• Wong SK, Chin KY, Suhaimi FH, Ahmad F, Ima-Nirwana S. the effects of a modified high-carbohydrate high-fat diet on metabolic syndrome parameters in male rats. Exp Clin Endocrinol Diabetes. 2018;126(4):205-212. doi:10.1055/s-0043-119352
• Brunt EM, Janney CG, Di Bisceglie AM, Neuschwander-tetri ba, bacon br. nonalcoholic steatohepatitis: a proposal for grading and staging the histological lesions. Am J Gastroenterol. 1999;94(9):2467-2474. doi:10. 1111/j.1572-0241.1999.01377.x
• Babacanoglu C, Yildirim N, Sadi G, Pektas MB, Akar F. Resveratrol prevents high-fructose corn syrup-induced vascular insulin resistance and dysfunction in rats. Food Chem Toxicol. 2013;60:160-167. doi:10.10 16/j.fct.2013.07.026
• Sadi G, Ergin V, Yilmaz G, et al. High-fructose corn syrup-induced hepatic dysfunction in rats: improving effect of resveratrol. Eur J Nutr. 2015;54(6):895-904. doi:10.1007/s00394-014-0765-1
• Ekici O, Aslan E, Guzel H, et al. Kefir alters craniomandibular bone development in rats fed excess dose of high fructose corn syrup. J Bone Miner Metab. 2022;40(1):56-65. doi:10.1007/s00774-021-01273-8
• Tsai SH, Tseng YH, Chiou WF, et al. The Effects of whole-body vibration exercise combined with an isocaloric high-fructose diet on osteoporosis and immunomodulation in ovariectomized mice. Front Nutr. 2022;9:915483. doi:10.3389/fnut.2022.915483
• Otani L, Nishi H, Koyama A, et al. Low-arginine and low-protein diets induce hepatic lipid accumulation through different mechanisms in growing rats. Nutr Metab (Lond). 2020;17:60. doi:10.1186/s12986-020-0 0477-5
• Meghelli-Bouchenak M, Belleville J, Boquillon M. Hepatic steatosis and serum very low density lipoproteins during two types of protein malnutrition followed by balanced refeeding. Nutrition. 1989;5(5): 321-329.
• Sousa RML, Ribeiro NLX, Pinto BAS, et al. Long-term high-protein diet intake reverts weight gain and attenuates metabolic dysfunction on high-sucrose-fed adult rats. Nutr Metab (Lond). 2018;15:53. doi:10.1186/s 12986-018-0290-y
• Wong SK, Chin KY, Suhaimi FH, Ahmad F, Ima-Nirwana S. Effects of metabolic syndrome on bone mineral density, histomorphometry and remodelling markers in male rats. PLoS One. 2018;13(2):e0192416. doi: 10.1371/journal.pone.0192416
• Bezerra RM, Ueno M, Silva MS, Tavares DQ, Carvalho CR, Saad MJ. A high fructose diet affects the early steps of insulin action in muscle and liver of rats. J Nutr. 2000;130(6):1531-1535. doi:10.1093/jn/130.6.1531
• Huang BW, Chiang MT, Yao HT, Chiang W. The effect of high-fat and high-fructose diets on glucose tolerance and plasma lipid and leptin levels in rats. Diabetes Obes Metab. 2004;6(2):120-126. doi:10.1111/j.1462- 8902.2004.00323.x
• Tsanzi E, Light HR, Tou JC. The effect of feeding different sugar-sweetened beverages to growing female Sprague-Dawley rats on bone mass and strength. Bone. 2008;42(5):960-968. doi:10.1016/j.bone.20 08.01.020
• Felice JI, Gangoiti MV, Molinuevo MS, et al. Effects of a metabolic syndrome induced by a fructose-rich diet on bone metabolism in rats. Metabolism. 2014; 63(2):296–305. doi:10.1016/j.metabol.2013.11.002
• Dupas J, Feray A, Goanvec C, et al. Metabolic syndrome and hypertension resulting from fructose enriched diet in wistar rats. Biomed Res Int. 2017;2017:2494067. doi:10.1155/2017/2494067
• Gancheva S, Zhelyazkova-Savova M. Are bisphosphonates associated with adverse metabolic and cognitive effects? a study in ıntact rats and rats fed high-fat high-fructose diet. Calcif Tissue Int. 2020;107(1):41-51. doi:10.1007/s00223-020-00684-5
• Pektas MB, Yücel G, Koca HB, et al. Dietary fructose-induced hepatic injury in male and female rats: influence of resveratrol. Drug Res (Stuttg). 2017;67(2):103-110. doi:10.1055/s-0042-118386
• Ackerman Z, Oron-Herman M, Grozovski M, et al. Fructose-induced fatty liver disease: hepatic effects of blood pressure and plasma triglyceride reduction. Hypertension. 2005;45(5):1012-1018. doi:10.1161/ 01.HYP.0000164570.20420.67