Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Abstract

In this study, a disease case caused with 15% mortality on gilthead sea bream (Sparus aurata) which was cultured in the Antalya Bay in the Mediterranean coast of Turkey was investigated in July 2015. Lethargy, loss of equilibrium, petechae on the operculums and the dorsal part of the body, pillar of the gills were recorded in the diseased fish. Internally, ascites, splenomegaly and hemorrhages on the intestinal tissue and muscle were observed. Occurrence of parasite was not detected. According to the results of phenotypical test and 16S rDNA sequencing analysis, the isolated bacterial species was identified as Staphylococcus hominis. Histopathologically, hyperplasia of primary lamellae, lamellar telangiectasia, edema and hemorrhages on the gill arch, numerous melanomacrophage centers (MMCs) in the spleen tissue, vacuoler degeneration, necrose and hemorrhages in the liver, multifocal necrosis, and numerous MMC in the kidney were determined and hemorrhages in the tunica propria region of the intestine were observed. It was determined that he isolates showed different susceptibility against antimicrobial agents. 

Keywords

• Gilthead sea bream
• Sparus aurata
• Cage Culture
• Staphylococcus hominis

References

1. Ahmet A, Rab A, Khan F & Qasim M (2011). Identification and antibiotic susceptibility of bacterial pathogens in rainbow trout at Juglote, Gilgit, Baltistan. Punjab University Journal of Zoology 26:133-137.
2. Alarcón J A, Magoulas A, Geogakopoulos T, Zouros E & Alvarez M C (2004). Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata). Aquaculture 230:65-80.
3. Altuğ G, Çardak M, Çiftçi P S & Gürün S (2013). First records and microgeographical variations of cultarable heterotrophic bacteria in an inner sea (the Sea of Marmara) between the Mediterranean and the Black Sea, Turkey. Turkish Journal of Biology 37:184-190.
4. Andrew J E, Bégout-Anras M L, Kadri S, Holm J & Huntingford F A (2003). Feeding responses of hatchery-reared gilthead sea bream (Sparus aurata L.) to a commercial diet and natural prey items. Marine and Freshwater Behaviour and Physiology 36: 77-86.
5. Austin B & Austin D (2012). Bacterial Fish Pathogens. Diseases of Farmed and Wild Fish, Springer Science-Business Media, England
6. Beraldo P & Canavase B (2011). Recovery of opercular anomalies in gilthead sea bream, Sparus aurata L: morphological and morphometric analysis. Journal of Fish Diseases 34:21-30.
7. Chu W-H & Lu C P (2005). Multiplex pcr assay fort he detection of pathogenic Aeromonas hydrophila. Journal of Fish Diseases 28:437-441. CLSI (M42-A) (2006). Clinical and Laboratory Standards Institute, Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals; approved guideline. Pennsylvania.
8. Collins R (1993). Principles of diagnosis.In: L Brown (Ed), Aquaculture for Veterinarians: Fish Husbandry and Medicine, Pergamon Press, Oxford, pp. 69-89.

9. Evans J J, Klesius P H, Gilbert P M, Shoemaker C A, Al Sarawi M A, Lansberg J, Duremdez R, Al Marzouk A & Al Zenki S (2002). Characterization of ß-haemolytic Streptococcus agalactiae in cultured sea bream, Sparus aurata L., and wild mullet, Liza klunzingeri (Day), in Kuwait. Journal of Fish Diseases 25:505-513.
10. García-Rosada E, Cano I, Martin-Antonio B, Labella A, Manchado M, Alonso M C & Castro D (2007). Co-occurence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish. International Microbiology 10:193-199
11. González-Párraga P, Cuesta A, Meseguer J & Ángeles-Esteban M (2011). Marine microorganisms: the world also changes. Science against microbial pathogens: communicating current research and technological advances. Farmatex : 1281-1292.
12. Gunn B A & Colwell R R (1983). Numerical taxonomy of Staphylococci isolated from the marine environment. International Journal of Systematic Bacteriology 33:751-759.
13. Hall T A (1999). BioEdit: a user-friendly biological alignment editor and analysis program for Windows 95/98/NT.
14. Kubilay A & Uluköy G (2004). First isolation of Staphylococcus epidermidis from cultured gilthead sea bream (Sparus aurata) in Turkey. Bulletin of the European Association of Fish Pathologists 24:137-143.
15. Liu P Y, Lin C F, Tung K C, Shyu C L, Wu M J, Liu J W, Chang C S, Chan K W, Huang J A & Shi Z Y (2013). Clinical and microbiological features of Shewanella bacterimia in patients with hepatobiliary disease. Internal Medicine 52:431-438.
16. Liu P Y, Shi Z Y, Shyu C L, Wu Z K, Lai K L, Chang C Y, Chen Y J, Huang J A, Mao Y C & Tung K C (2014). Cobra bite wound infection caused by Shewanella algae. International Journal of Infectious Diseases: e11-e12.
17. Mladineo I (2006). Check list of the parasitofauna in Adriatic sea cage-reared fish. Acta Veterinaria Belgrade 56: 285-292.
18. Moustafa M, Eissa A E, Laila A M, Gaafar A Y, Abumourad I M K & Elgendy M Y (2015). Investigations into potential causes of mass kills in mari-cultured gilthead sea bream (Sparus aurata) at Northern Egypt. Research Journal of Pharmaceutical, Biological and Chemical Sciences 6(1):466-477.
19. Noyo M & Lamas J (1996). Morphology and histochemistry of a PAS-positive granular cell in the gills of the gilthead sea bream, Sparus aurata L. Journal of Anatomy 189: 439-443.
20. Padilla D, Acosta F, Rodriguéz F, Gómez V, Déniz S & Real F (2001). Mortality caused by Staphylococcus xylosus in red porgy (Pagrus pagrus) fish. Journal of Applied Animal Research 19:203-208.
21. Scarano C, Spanu C, Ziino G, Pedonese F, Dalmasso A, Spanu V, Virdis S & De Santis E P L (2014). Antibiotic resistance of Vibrio species isolated from Sparus aurata reared in Italian mariculture. New Microbiologica 37: 327-329.
22. Schleifer K H & Bell J A (2009). Family VIII. Staphylococcaceae fam. nov. In: G Garrity, D Jones, N R Krieg, W Ludwig, F A Rainey, K H Schleifer & W B Whitman (Eds), Bergey’s Manual of Systematic Bacteriology, Springer-Verlag, New York, pp. 392.
23. Seeley W, Vandemark P J & Lee J L (1991). Microbes in Action, A Laboratory Manual Microbiology, W H Freeman and Company, New York
24. Sitti-Zahrah A, Shahidan H, Wan Norazian G, Noor Amal A M, Nur-Nazifah M & Misri S (2008). Isolation of Staphylococcus spp. in cage cultured tilapia of different water bodies. NaFIS Symposium Perikanan Kebangsaan: 14-16 Julai, Kuala, (unpublished).
25. Tanokhy M (2013). Some study on bacterial infection in some cultured marine fish. Journal of the Arabian Aquaculture Society 8:163-178.
26. Toranzo A E, Magariños B & Romalde J L (2005). A review of the main bacterial fish diseases in mariculture systems. Aquaculture 246: 37-61.
27. Uddin G M N, Larsen M H, Guardabassi L & Dalsgaard A (2013). Bacterial flora and antimicrobial resistance in raw frozen cultured seafood imported to Denmark. Journal of Food Protection 76: 490-499.
28. Yiagnisis M & Athanassopoulou F (2011). Bacteria isolated from diseased wild and farmed marine fish in Greece.F Aral (Ed), Recent Advances in Fish Farms, 153-168.
29. Zhang L, Thomas J C, Miragaia M, Bouchami O, Chaves F, D´Azevedo P A, Aanensen D M, De Lencastre H, Garry B M & Robinson D A (2013). Multilocus sequence typing and further genetic characterization of the enigmatic characterization of the enigmatic pathogen, Staphylococcus hominis, PLoSOne 8:e66496.
30. Zlotkin A, Hershko H & Eldar A (1998). Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Applied and Enviromental Microbiology 64:4065-4067.