Polymorphisms of TLR1, TLR4 and SLC11A1 Genes in Some Cattle Breeds Reared in Turkey

Abstract

The objective of this study was to examine the allelic and genotypic profiles of four SNPs of bovine Toll-Like receptor 1 (TLR1), Toll-Like Receptor 4 (TLR4) and Solute Linkete Carier 11A1 (SLC11A1) genes in Holstein, two Turkish native breeds and their crossbreds in Turkey. For this purpose, a total of 1023 cattle from Holstein (HL, n= 410), Anatolian Black (AB, n= 106), East Anatolian Red (EAR, n= 84), Anatolian Black Crossbreed (ABC, n= 124) and East Anatolian Red crossbreed (EARC, n= 299) breeds were examined for four SNPs. Samples were genotyped using by the PCR-RFLP method. According to the TLR1 (+1380) SNP, EAR was in Hardy-Weinberg equilibrium (HWE) while the AB, HL and native crossbreeds were deviated from HWE. All cattle breeds were in HWE for the TLR1 (+1596) SNP. In terms of TLR4 (+10) SNP, the CC genotype had the highest frequency in HL and native crossbreeds whereas the AB and EAR breeds were monomorphic and only CC genotype was found these breeds. According to the SLC11A1 (+1066) SNP, the CC genotype had the highest while the GG genotype frequency the lowest in all breeds. In addition EAR breed was in HWE while the AB, HL and native crossbreeds deviated from HWE.

Keywords

• Polymorphism
• Turkish native cattle
• Toll-like receptor
• SLC11A1
• Marker-assisted selection

References

1. Brightbill H D, Libraty D H, Krutzik S R, Yang R B, Belisle J T , Bleharski J R, Maitland M, Norgard M V, Plevy S E, Smale S T, Patrick J B, Barry R B, Paul J G & Robert L M (1999). Host Defense Mechanisms Triggered By Microbial Lipoproteins Through Toll-Like Receptors. Science 285: 732–736
2. Buwitt-Beckmann U, Heine H, Wiesmuller K H, Jung G, Brock R, Akira S & Ulmer A J (2006). Tlr1- And Tlr6-Independent Recognition Of Bacterial Lipopeptides. The Journal Of Biological Chemistry 281: 9049–9057
3. Chang J S, Huggett J F, Dheda K, Kim L U, Zumla A & Rook G A (2006). Myobacterium Tuberculosis Induces Selective Up-Regulation Of Tlrs In The Mononuclear Leukocytes Of Patients With Active Pulmonary Tuberculosis. The Journal Of Immunology 176: 3010–3018
4. Egger-Danner C, Cole J B, Pryce J E, Gengler N, Heringstad B, Bradley A & Stock K F (2015). Invited Review: Overview Of New Traits And Phenotyping Strategies In Dairy Cattle With A Focus On Functional Traits. Animal 9(2): 191–207
5. Hawn T R, Misch E A, Dunstan S J, Thwaites G E, Lan N T, Quy H T, Chau T T, Rodrigues S, Nachman A, Janer M, Hien T T, Farrar J J & Aderem A (2007). A Common Human Tlr1 Polymorphism Regulates The Innate Immune Response To Lipopeptides. European Journal Of Immunology 37: 2280–2289
6. Kumar N, Ganguly I, Singh R, Deb S M, Kumar S, Sharma A & Mitra A (2011). Dna Polymorphism In Slc11a1 Gene And Its Association With Brucellosis Resistance In Indian Zebu (Bos Indicus) And Crossbred (Bos Indicus×Bos Taurus) Cattle. Asian-Australasian Journal Of Animal Sciences 24 (7): 898 - 904
7. Le Roex N, Koets A P, Van Helden P D & Hoa E G (2013). Gene Polymorphisms In African Buffalo Associated With Susceptibility To Bovine Tuberculosis Infection. Plos One 8(5): E64494
8. Novák K (2014). Functional Polymorphisms İn Toll-Like Receptor Genes For İnnate İmmunity İn Farm Animals. Veterinary Immunology And Immunopathology 157(1): 1-11

9. Mucha R, Bhide M R, Chakurkar E B, Novak M & Mikula S R I (2009). Toll-Like Receptors Tlr1, Tlr2 And Tlr4 Gene Mutations And Natural Resistance To Mycobacterium Avium Subsp. Paratuberculosis Infection In Cattle. Veterinary Immunology And Immunopathology 128: 381–388
10. Ogorevc J, Kunej T, Razpet A & Dovc P (2009). Database Of Cattle Candidate Genes And Genetic Markers For Milk Production And Mastitis. Animal Genetics 40: 832-851
11. Prakash O, Kumar A, Sonwane A, Rathore R, Singh R V, Chauhan A, Kumar P, Renjith R, Yadav R, Bhaladhare A, Baqir M & Sharma D (2014). Polymorphism Of Cytokine And Innate Immunity Genes Associated With Bovine Brucellosis In Cattle. Molecular Biology Reports 41: 2815–2825
12. Russell C D, Widdison S, Leigh J A & Coffey T J (2012). Identification Of Single Nucleotide Polymorphisms In The Bovine Toll-Like Receptor 1 Gene And Association With Health Traits In Cattle. Veterinary Research 43(1): 17
13. Sambrook J, Fritsch E F & Maniatis T (1989) - Molecular Cloning: A Laboratory Manual (2nd Ed.), Cold/Spring Harbor, New York Schwartz D A (2002). The Genetics Of Innate Immunity. Chest 121: 62–68
14. Strapáková E, Candrák J & Strapák P (2016). Genetic Relationship Of Lactation Persistency With Milk Yield, Somatic Cell Score, Reproductive Traits, And Longevity In Slovak Holstein Cattle. Archives Animal Breeding 59: 329–335
15. Sun L, Song Y, Riaz H, Yang H, Hua G, Guo A & Yang L (2012). Polymorphisms In Toll-Like Receptor 1 And 9 Genes And Their Association With Tuberculosis Susceptibility In Chinese Holstein Cattle. Veterinary Immunology And Immunopathology 147: 195– 201
16. Takeda K & Akira S (2001). Roles Of Toll-Like Receptors In Innate Immune Responses. Genes Cells 6: 733-742
17. Uciechowski P, Imhoff H, Lange C, Meyer C G, Browne E N, Kirsten D K, Schröder A K, Schaaf B, Al-Lanham A, Reinert R R, Reiling N, Haase H, Hatzmann A, Fleischer D, Heussen N, Kleines M & Rink L (2011). Susceptibility To Tuberculosis Is Associated With Tlr1 Polymorphisms Resulting In A Lack Of Tlr1 Cell Surface Expression. Journal Of Leukocyte Biology 90: 377-388
18. Underhill D M, Ozinsky A, Smith K D & Aderem A (1999). Toll-Like Receptor-2 Mediates Mycobacteria-Induced Proinflammatory Signaling In Macrophages. Proceedings Of The National Academy Of Sciences 96: 14459–14463