Investigation of Genotoxic, Antimicrobial and Antioxidant Activities of Leaf and Flower Extracts of Cynara syriaca Boiss

Abstract

The mutagenicity and antimutagenicity of leaves and flower extract of Cynara syriaca Boiss were studied with Ames assay in Salmonella typhimurium TA98 and TA100 strains. While leaves extract did not show any mutagenic effects against all the tester strains with or without metabolic activation, the flower extract showed mutagenic effect against TA98 strain without metabolic activation. On the other hand, it has been observed that the extracts have antimutagenic activity against mutations induced by sodium azide and daunomycin. The antimicrobial activity of extracts was determined by disc diffusion and MIC value. Both of the extracts possess weak antimicrobial activity. Cupric reducing antioxidant capacity (CUPRAC), DPPH free radical scavenging activity, and ABTS radical cation decolorization methods were carried out to determine the antioxidant activity. Among the tested antioxidant methods, the highest antioxidant capacity was determined in ABTS radical cation decolorization assay in which both of the extracts exhibited the best effect. Flower extract exhibited higher activity also in DPPH free radical scavenging.

Keywords

• Mutagenicity,Antimutagenicity,Antioxidant activity,Antimicrobial Activity,Cynara syriaca

References

1. Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26): 7970-7981.
2. Báidez, A. G., Gómez, P., Del Río, J. A., & Ortuño, A. (2007). Dysfunctionality of the xylem in Olea europaea L. plants associated with the infection process by Verticillium dahliae Kleb. Role of phenolic compounds in plant defense mechanism. Journal of agricultural and food chemistry, 55(9): 3373-3377.
3. Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-1200.
4. Brown, J. E., & Rice-Evans, C. A. (1998). Luteolin-rich artichoke extract protects low density lipoprotein from oxidation in vitro. Free radical research, 29(3): 247-255.
5. Caderni, G., De Filippo, C., Luceri, C., Salvadori, M., Giannini, A., Biggeri, A., & Dolara, P. (2000). Effects of black tea, green tea and wine extracts on intestinal carcinogenesis induced by azoxymethane in F344 rats. Carcinogenesis, 21(11): 1965-1969.
6. Chulasiri, M. (1998). Mutagenicity and antimutagenicity of flavonoids extracted from Millingtonia hortensis L. The Journal of toxicological sciences, 23: 224-228.
7. Han, X., Shen, T., & Lou, H. (2007). Dietary polyphenols and their biological significance. International Journal of Molecular Sciences, 8(9): 950-988.
8. Heidari, R., Siami, A., & Monazami, C. (2000). Lipid content and fatty acid composition in some species of Compositae family. Iran Agricultural Research, 19(1): 41-48.

9. Huang, W.-Y., Cai, Y.-Z., & Zhang, Y. (2009). Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and cancer, 62(1): 1-20.
10. Ikken, Y., Morales, P., Martín ez, A., Marín, M. L., Haza, A. I., & Cambero, M. I. (1999). Antimutagenic effect of fruit and vegetable ethanolic extracts against N-nitrosamines evaluated by the Ames test. Journal of agricultural and food chemistry, 47(8): 3257-3264.
11. Kukić, J., Popović, V., Petrović, S., Mucaji, P., Ćirić, A., Stojković, D., & Soković, M. (2008). Antioxidant and antimicrobial activity of Cynara cardunculus extracts. Food chemistry, 107(2): 861-868.
12. Kuo, M.-L., Lee, K.-C., & Lin, J.-K. (1992). Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, ellagic acid and indole-3-carbinol in the Salmonella and CHO systems. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 270(2): 87-95.
13. Kupicha, F., & Davis, P. (1975). Cynara L. Flora of Turkey and the East Aegean Islands, 5: 327-329.
14. Lin, H. S., Yue, B. D., & Ho, P. C. (2009). Determination of pterostilbene in rat plasma by a simple HPLC‐UV method and its application in pre‐clinical pharmacokinetic study. Biomedical Chromatography, 23(12): 1308-1315.
15. Maron, D. M., & Ames, B. N. (1983). Revised methods for the Salmonella mutagenicity test. Mutation Research/Environmental Mutagenesis and Related Subjects, 113(3-4): 173-215.
16. Meriçli, A., & Seyhan, G. (2006). Constituents of Cynara syriaca. Leaves. Pharmaceutical biology, 44(9): 643-645.
17. Moreno, M. a. I. N., Isla, M. a. I., Sampietro, A. R., & Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71(1): 109-114.
18. NCCLS (National Committee for Clinical Laboratory Standards). (2009). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard 8th ed. M08-A8.Wayne Pa.
19. Nielsen, S., Young, J. F., Daneshvar, B., Lauridsen, S., Knuthsen, P., Sandström, B., & Dragsted, L. O. (1999). Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects. British Journal of Nutrition, 81(6): 447-455.
20. Orhan, F., Barış, Ö., Yanmış, D., Bal, T., Güvenalp, Z., & Güllüce, M. (2012). Isolation of some luteolin derivatives from Mentha longifolia (L.) Hudson subsp. longifolia and determination of their genotoxic potencies. Food chemistry, 135(2): 764-769.
21. Owen, R., Giacosa, A., Hull, W., Haubner, R., Spiegelhalder, B., & Bartsch, H. (2000). The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. European Journal of Cancer, 36(10): 1235-1247.
22. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9): 1231-1237.
23. Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine, 20(7): 933-956.
24. Slanina, J., Paulová, H., Humpa, O., Bochořáková, H., & Táborská, E. (1999). 1, 5-dicaffeoylquinic acid, an antioxidant component of Cynara cardunculus leaves. Scripta Medica (BRNO), Brno: Lékařská fakulta Masarykovy univerzity, 72: 1211-3395.
25. Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1): 49-55.
26. Sun, A. Y., Wang, Q., Simonyi, A., & Sun, G. Y. (2011). Herbal Medicine and Clinical Aspects,. In: Iris, F.F. Benzie & Sissi Wachtel-Galor (Eds), Botanical Phenolics and Neurodegeneration. CRC Press, 315-325.
27. Veeriah, S., Kautenburger, T., Habermann, N., Sauer, J., Dietrich, H., Will, F., & Pool‐Zobel, B. L. (2006). Apple flavonoids inhibit growth of HT29 human colon cancer cells and modulate expression of genes involved in the biotransformation of xenobiotics. Molecular carcinogenesis, 45(3): 164-174.
28. Yamada, J., & Tomita, Y. (1996). Antimutagenic activity of caffeic acid and related compounds. Bioscience, biotechnology, and biochemistry, 60(2): 328-329.