Efficacy of Native Entomopathogenic Nematodes on the Larvae of Tomato Leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Abstract

This study was conducted to determine the efficacy of native entomopathogenic nematodes (EPNs); Steinernema affine 46 (Bovien, 1937), S. feltiae 879 (Filipjev, 1934), S. carpocapsae 1133 (Weiser, 1955) and Heterorhabditis bacteriophora 1144 (Poinar, 1976) on the larvae of tomato leafminer Tuta absoluta (Meyrick). Bioassays were conducted in the laboratory at four different temperatures (10, 15, 20 and 25±1 oC) in the plates and 30 infective juveniles (IJs) were inoculated to a single T. absoluta larva for each nematode species. After nematode inoculation, larvae were checked on the 3rd, 5th, 7th days and mortalities were recorded. All nematode species used in the study showed the lowest efficacy on the 3rd control day at 10 oC and the highest efficacy on the 7th day at 25 oC. S. feltiae 879 was found as the most efficient species with the highest mortality (91.67%) among EPNs used in the study. The results proved that T. absoluta larvae are highly susceptible to EPNs and the control of the pest by EPNs on this stage is successful.

Keywords

• Tomato,Tuta absoluta,Larva,Biological control,Entomopathogenic nematodes

References

1. Batalla-Carrera L, Morton A & Garcia-del-pino F (2010). Efficacy of entomopathogenic nematodes against the tomato leafminer Tuta absoluta in laboratory and greenhouse conditions. Biocontrol 55: 523-530
2. Bedding R A & Akhurst R J (1975). A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21: 109-110
3. Ben-Husin T O A (2017). Biological control of tomato leaf miner Tuta absoluta using entomopathogenic nematodes. PhD Thesis, Faculty of Science, Agriculture and Engineering, Newcastle University, United Kingdom, pp. 196.
4. Boemare N (2002). Biology, taxonomy and systematic of Photorhabdus and Xenorhabdus. In: R. Gaugler (Ed.), Entomopathogenic Nematology. Wallingford, UK: CAB International, pp. 35-56
5. Cáceres S (1992). La polilla del tomate en Corrientes. Biología y control, Estación Experimental Agropecuaria Bella Vista INTA, pp. 19. 11: 1-42
6. Desneux N, Wajnberg E, Wyckhuys K A G, Burgio G, Arpaia S, Narvaez-Vasquez C A, Gonzalez-Cabrera J, Ruescas D A, Tabone E, Frandon J, Pizzol J, Poncet C, Cabello T, Urbaneja A 2010. Biological invasion of European tomato crops by Tuta absoluta: Ecology, geo-graphic expansion and prospects for biological control. Journal of Pest Science 83: 197-215
7. Estay P (2000). Polilla del tomate Tuta absoluta (Meyrick), URL http://alerce.inia.cl/docs/Informativos/Informativo09.pdf (Date accessed: September 2014)
8. Garcia-del-Pino F, Alabern X & Morton A (2013). Efficacy of soil treatments of entomopathogenic nematodes against the larvae, pupae and adults of Tuta absoluta and their interaction with the insecticides used against this insect. BioControl 58(6), 723-731

9. Gözel U & Güneş Ç (2013). Effect of entomopathogenic nematode species on the corn stalk borer (Sesamia cretica Led. Lepidoptera: Noctuidae) at different temperatures. Turkish Journal of Entomology 37(1): 65-72
10. Gözel Ç & Kasap İ (2015). Efficacy of entomopathogenic nematodes against the tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato field. Turkish Journal of Entomology 39(3): 229-237
11. Gözel Ç (2016). Domates Güvesi Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nin Mücadelesinde Entomopatojen Nematodların Kullanım Olanakları. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, 83 s
12. Kamali S, Karimi J & Koppenhöfer A M (2018). New Insight into the Management of the Tomato Leaf Miner, Tuta absoluta (Lepidoptera: Gelechiidae) with Entomopathogenic Nematodes. Journal of Economic Entomology 111(1): 112-119
13. Kasap İ, Gözel U & Özpınar A (2011). “Yeni bir zararlı; Domates güvesi Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), 284”. Çanakkale Tarımı Sempozyumu (Dünü, Bugünü, Geleceği) Bildirileri, Çanakkale Onsekiz Mart Üniversitesi Ziraat Fakültesi, Çanakkale pp: 717
14. Kaya H K & Gaugler R (1993). Entomopathogenic nematodes. Annual Review of Entomology, 38: 181-206
15. Kaya H K & Stock S P (1997). “Techniques in Insect Nematology, 281-324” In: L A Lacey (Ed.), Manual of Techniques in Insect Pathology Biological Techniques Series, Academic Press, San Diego, California pp: 409
16. Kılıç T (2010). First record of Tuta absoluta in Turkey. Phytoparasitica 38: 243-244
17. Moore J E (1983). Control of tomato leafminer (Scrobipalpula absoluta) in Bolivia. Tropical Pest Management 29: 231-238
18. Olthof ThHA & Broadbent A B (1990). “Control of a chrysanthemum leafminer, Liriomyza trifolii with the entomophilic nematode, Heterorhabditis heliothidis, 379”. Abstracts of Papers, Posters and Films Presented at the Second International Nematology Congress, 11-17 August, Veldhoven the Netherlands, Nematologica 36(1990): 327-403
19. Roditakis E, Papachristos D & Roditakis N E (2010). Current status of the tomato leafminer Tuta absoluta in Greece. EPPO Bulletin 40: 163-166
20. Shamseldean M, Abd-Elbary N A, Shalaby H & Ibraheem H I H (2014). Entomopathogenic nematodes as biocontrol agents of the tomato leaf miner Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on tomato plants. Egyptian Society for Biological Control of Pests, 24: 503-513
21. Siqueira H A A, Guedes R N C & Picanço M C (2000). Insecticide resistance in populations of Tuta absoluta (Lepidoptera: Gelechiidae). Agricultural and Forest Entomology 2: 147-153
22. Susurluk A & Ehlers R U (2008). Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl, 53: 627-641
23. Türköz S & Kaşkavalcı G. (2016). Determination of the efficacy of some entomopathogenic nematodes against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory conditions. Turkish Journal of Entomology 40(2): 175-183
24. Urbaneja A, Vercher R, Navarro V, Porcuna J L & Maria F G (2007). La polilla del tomate, Tuta absoluta. Phytoma España 194: 16-23
25. Van Damme V M, Beck B K, Berckmoes E, Moerkens R, Wittemans L, Vis R D, Nuyttens D, Casteels H F, Maes M, Tirry L, Clercq P D (2016). Efficacy of entomopathogenic nematodes against larvae of Tuta absoluta in the laboratory. Pest Management Science 72: 1702-1709
26. Vargas H C (1970). Observaciones sobre la biologia y enemigos naturales de la polilla del tomate, Gnorimoschema absoluta (Meyrick) (Lepidoptera: Gelechiidae). Idesia 1: 75-110
27. White G F (1927). A method for obtaining infective nematode larvae from cultures. Science 66: 302-303