Palynological Study on Some Grape (Vitis vinifera L.) Cultivars Using Scanning Electron Microscopy

Abstract

Pollen is an important morphological parameter for cultivar identification. This is of great importance in detailed investigations by scanning electron microscope (SEM). In this study, the pollen morphology of selected grape cultivars was examined by SEM. The pollen length, width, P/E ratio of pollen and features of surface were observed. The pollen differed in some microstructural characteristic. Pollen width exhibited significant according to the varieties (10.12-22.44 μm). Similarly, the statistical difference occurred among the thirty Vitis cultivars in terms of mean pollen length (16.26-29.65 μm). Areolat pollen was determined in some cultivars. Depending on the cultivars there was significant differences in terms of pores diameter. According to PCA performed in 30 grape cultivars, 3 principal components were revealed and they defined 94.98% of the variance. Cultivars were divided into groups according to pollen features on the cluster. Consequently, the cultivars were categorized under two main groups. The present research is a contribution to a more detailed analysis of grapevine cultivars.

Keywords

• pollen
• morphology
• scanning electron microscope (SEM)
• palynology
• classification
• description

References

1. Referans1 Ahmedullah M (1983). Morphology of pollen from selected Vitis cultivars. J. Am. Soc. Hortic. Sci. 108: 201-206.
2. Referans2 Ben Slimane M, Askri F (1990). La taille des grains de pollen comme moyen d’identification de souches de vignes tunnisiennes. Bull Organ Int Vigne Vin 63: 899–909.
3. Referans3 Cabello F, S Santa Maria, P de Luis Villota, ME Tortosa Tortola (1994). Palynological study of the pollen grain of Vitis vinifera L. cultivars. Some aspects of sculpturing and pollination. Vitis, 33(2): 57-61
4. Referans4 Eichhorn KW, Lorenz H (1977). Phaenologische entwicklungstadien der rebe. Nachrichtenblatt des deutschen pflanzenschutzdienstes (Braunschweig). 29, 119-120. Faegri K. Iversen J. 1989. Textbook of Pollen Analysis. 328 pp. John Wiley & Sons Ltd., Chichester.
5. Referans5 Erdtman GE (1952). Pollen Morphology and Plant Taxonomy. Angiosperms. (An Introduction to Palynology I.). 539 pp. Almquist and Wiksell (Stockholm), USA.
6. Referans6 Faegri K, Iversen J (1989). Textbook of Pollen Analysis. 328 pp. John Wiley & Sons Ltd., Chichester.
7. Referans7 Gallardo A, R Ocete R, Ángeles López M. Lara M, Rivera D (2009). Assessment of pollen dimorphism in populations of Vitis vinifera L. subsp. sylvestris (Gmelin) Hegi in Spain. Vitis, 48(2): 59–62.
8. Referans8 Gökbayrak Z, Engin H (2016). Micromorphology of pollen grains of some grape cultivars. VII International Scientific Agriculture Symposium “Agrosym 2016”. Book of Proceedings. 176-180.

9. Referans9 Hyde HA, Adams KF (1958). An Atlas of Airborne Pollen Grains. 116 pp. Macmillan & Co., Ltd., London.
10. Referans10 Inceoglu O, Pınar N, Oybak-Dönmez E (2000). Pollen morphology of wild Vitis sylvestris Gmelin (Vitaceae). Turk. J. Bot. 24: 147-150.
11. Referans11 Jovanovic-Cvetkovic T, Micic N, Djuric G, Miljan Cvetkovic M (2016). Pollen Morphology and Germination of Indigenous Grapevine Cultivars Žilavka and Blatina (Vitis vinifera L.). AgroLife Scientific Journal, 5(1): 105-109.
12. Referans12 Lombardi JA (2007). Systematic of Vitaceae in South America. Canadian Journal of Botany, 85: 712- 721.
13. Referans13 Mabberley DI (1987). The Plant Book. Camb. Univ. Press, Cambridge, New York.
14. Referans14 Marasalı B (2005). Palynological Study on the Pollen Grains of Selected Turkish Grape (Vitis vinifera L.) Cultivars. Turk J Agric For., 29: 75-81.
15. Referans15 Martens MHR, Reisch BI, Mauaro MC (1989). Pollen size variability within genotypes of Vitis. HortScience, 24: 659-662.
16. Referans16 Najmaddin C, Hussin K, Maideen H (2013). Comparative leaf anatomical of selected species in Vitaceae and Leeaceae. American Journal of Applied Sciences, 10(4): 414-417.
17. Referans17 Reille M (1966). Contribution a I'etude palynologique de la famille de Vitaceae. Memoire Diplome Etudes Supereures. 88 pp. Montpellier
18. Referans18 Roytchev V,Terziisky D, Dimova D, Karageorgiey S (1994). Scanning electron microscopy study of pollen morphology in seedless grape (Vitis vinifera L.) cultivars. Vitis, 33: 105-108.
19. Referans19 Roytchev V (1995). Palynobiometric studies in vines (Vitis vinifera L.). Vitis, 34: 197-200.
20. Referans20 Roytchev V (1997). Palynological Classification of Seedless Grape (Vitis vinifera L.) Cultivars. Am. J. Enol. Vitic., 48(2): 207-213.
21. Referans21 Storey WB (1975). Figs. In: Editors Janick J and Moore JN. Advances in fruit breeding. 568-589.
22. Referans22 Kharitonashyili LA, Ramishyili RM, Kipliani MG (1989). Study of the pollen in various types on Vitis vinifera L. flowers using a scanning electron microscope. Soobshch. Akad. Nauk Gruz. SSR 136: 653-656.
23. Referans23 Tanaka N, Uehara K, Murata J (2004). Correlation between pollen morphology and pollination mechanisms in the Hydro charitaceae. J Plant Res. 117, 265–276.
24. Referans24 Uzun HI, Ilter E (1987). Studies on Vitis pollen using scanning electron microscopy. Doga, Türk Tarim Ormancilik Derg., 11, 457-460.
25. Referans25 Van Der Pluym A, Hideux M (1977). Application d’une methodologic quantitative á la palynologic d’ Eryngiummaritimum (Umbelliferae). Plant. Syst. Evol., 127: 55-85.
26. Referans26 Wang G, Chen J, Li ZB, Zhang FP, Yang DR (2014). Has pollination mode shaped the evolution of Ficus pollen? PLoS ONE 9(1): e86231. doi:10.1371/ journal.pone.0086231.
27. Referans27 Wodehouse RP, 1935. Pollen Grains, Their Structure, Identification and Significance in Science and Medicine. 574 pp. McGraw-Hill Book Company Inc., New York and London.