Taze Çay Yaprağı ve Yeşil Çayın Ekstrakt ve Fraksiyonlarının Antioksidan ve Antibakteriyel Etkileri

Abstract

Taze çay yaprağı FTL ve yeşil çayın GT metanol, etanol ve su ham ekstraktları ile bu ekstraktların etil asetat ve su fraksiyonlarının polifenol içeriği, polifenol verimi, antioksidan ve antimikrobiyel aktiviteleri belirlenmiştir. FTL ve GT örneklerinde etanol ekstraktlarının etil asetat fraksiyonunun en yüksek düzeyde polifenol içerdiği sırasıyla 680.2 ve 560.8 mg gallik asit eşdeğeri g-1 belirlenmiştir. Her üç ekstraksiyon solventinde de etil asetat fraksiyonu en yüksek polifenol içeriğine sahiptir. En yüksek antioksidatif aktiviteyi FTL ve GT’nın su ekstraktlarının etil asetat fraksiyonu göstermiştir sırasıyla 80.8 ve 78.6 g askorbik asit eşdeğeri 100 g-1 kuru ekstrakt . FTL ve GT örneklerinin antioksidan aktivitesi ile toplam fenolik madde miktarı arasında oldukça yüksek sırasıyla R2 = 0.9376 ve 0.9783 bir korelasyon mevcuttur. Her iki çay örneğinin etil asetat fraksiyonu S. aureus ve B. cereus üzerinde antibakteriyel etki gösterirken FTL’nin ham ekstraktı S. aureus üzerinde etki göstermiştir

Keywords

• Çay
• ekstraksiyon
• fraksiyon
• polifenoller
• antioksidan
• antibakteriyel

Antioxidant and Antibacterial Activities of Various Extracts and Fractions of Fresh Tea Leaves and Green Tea

Abstract

Polyphenol content, polyphenol yield, antioxidant and antibacterial activities of methanol, ethanol and water extracts and their crude, ethyl acetate and water fractions derived from fresh tea leaves FTL and green tea GT were evaluated. Ethyl acetate fractions of ethanolic extracts contained the highest polyphenol content in FTL and GT samples 680.2 and 560.8 mg gallic acid equivalent g-1 dry extract, respectively . For each of 3 extraction solvents, ethyl acetate fractions always had highest polyphenol content, for both teas. Ethyl acetate fractions of water extracts of FTL and GT had highest antioxidant activity 80.8 and 78.6 g ascorbic acid 100g-1 dry extract . A rather high correlation coefficient R2 = 0.9376 for FTL and 0.9783 for GT was obtained between antioxidant activity to total polyphenol content for both tea. Ethyl acetate fractions of extracts showed antibacterial activity on S. aureus and B. cereus. Crude extract of FTL were also effective on S. aureus

Keywords

• Tea
• extraction
• fraction
• polyphenols
• antioxidant
• antibacterial

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