Morphological and Anatomical Investigations on Old Home x Farmingdale 333 / Pyrus elaeagrifolia in Vitro Micrografts

Year 2014, Volume: 20 Issue: 3, 269 - 279, 14.08.2014

Hatice Dumanoğlu , Aysun Çelik Nurhan Büyükkartal , Saeed Dousti

https://doi.org/10.15832/tbd.65549

Cited By: 3

Abstract

In this study, the possibility of creation of a specific pear rootstock combination by micrografting was investigated. Two genotypes; ‘Old Home x Farmingdale 333’ (Pyrus communis L.) which is a clonal semi dwarf pear rootstock resistant to fireblight and pear decline, and Pyrus elaeagrifolia known as tolerant to Fe-chlorosis, salinity and drought stresses were used to obtain a clonal, semi dwarf and tolerant to the stresses pear rootstock combination. In vitro P. elaeagrifolia seedlings were used as rootstock, and in vitro shoots of ‘OH x F 333’ were used as semi dwarf interstock. Cleft grafting was applied in micrografts. Grafted seedlings were cultured on Murashige and Skoog basal medium with ½ strength of macronutrients for 8 weeks. Cultures were incubated under white fluorescent light, 16 h per day. However, complete darkness was applied for 1 or 2 weeks at the beginning of incubation for the darkness treatments. Graft take success in the control treatment was significantly higher (97.9%) than darkness treatments (90.5% and 82.5%, respectively). Ultrastructural observations with transmission electron microscope revealed that dividing cambial initials reached to 2-3 layers and new xylem and phloem elements distinctly differentiated in transversal sections of the graft union after 8 weeks of micrografts in the control and dark treatments. The results indicated that a successful graft union formation occurred. To the best of our knowledge, this is the first report of in vitro micrografting on Pyrus elaeagrifolia Pallas.

Keywords

Micrografting; Graft union; Interstock; Dark treatment

Morphological and Anatomical Investigations on in Vitro Micrografts of OHxF 333 / Pyrus elaeagrifolia Interstock / Rootstock Combination in Pears

Abstract

In this study, possibility of creating a specific interstock / rootstock combination to obtain a clonal, semi dwarf, composite pear rootstock tolerant to various stresses by micrografting was investigated. In vitro shoots of ‘Old Home x Farmingdale 333’ (Pyrus communis L.) which is a clonal semi dwarf pear rootstock resistant to fireblight and pear decline was used as interstock, and in vitro Pyrus elaeagrifolia Pallas seedlings known as tolerant to Fe-chlorosis, salinity and drought stresses was used as rootstock. Cleft grafting was applied in micrografts. Grafted seedlings were cultured on Murashige and Skoog basal medium with ½ strength of macronutrients for 8 weeks under white fluorescent light for 16 h day. Cultures, except the control, received complete darkness either 1 or 2 weeks at the beginning of incubation. Graft take success in the control treatment was significantly higher (97.9%) than darkness treatments of 1 or 2 weeks (90.5% and 82.5%, respectively). Ultrastructural observations with transmission electron microscope revealed that dividing cambial initials reached to 2-3 layers, and new xylem and phloem elements distinctly differentiated in transverse sections of the graft union 8 weeks after micrografting in the control and darkness treatments. The results indicated a successful graft union formation.

Keywords

Micrografting; Graft union; Interstock; Darkness treatment

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