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Ege Bölgesi Yerel Asma Çeşitlerinin (Vitis vinifera L.) Basit Tekrar Dizileri (SSRs) ile Karakterizasyonu

Yıl 2015, Cilt: 21 Sayı: 4, 538 - 545, 15.12.2015
https://doi.org/10.1501/Tarimbil_0000001355

Öz

Türkiye᾽nin Ege bölgesinde yetiştirilen otuz altı yerel asma çeşidi ile standart çeşitlerden Cabernet Sauvignon ve Merlot
arasındaki genetik ilişkiler SSR markörleri kullanılarak belirlenmiştir. Kullanılan 11 SSR primeri başarılı amplifikasyonlar
vermiş ve toplam 37 polimorfik bant üretmiştir. Bant sayısı primer başına 2 ile 6 arasında ve polimorfik bant sayısı 2
ile 3.6 arasında değişmiştir. Genotipler arasındaki Dice genetik benzerlik katsayıları 0.296 ile 0.882 arasında değişim
göstermiştir. UPGMA dendrogramında iki ana grup ortaya çıkmıştır. Birinci grup Sultan Dimriti, Veyis ve Güvercin
Gözü çeşitlerinden oluşmuştur. Yerel çeşitlerin büyük kısmının yer aldığı ikinci grup çok sayıda alt gruptan oluşmuştur.
Yerel asma çeşitleri ve referans çeşitler arasındaki genetik farklılıklar açıkça gözlenmiştir. En yüksek (0.96) genetik
benzerliğin Siyah Razakı ve Parmak çeşitleri arasında olduğu belirlenmiştir. İnek Memesi ve Ufak Dimrit çeşitlerinin
birbirinin sinonimi olduğu tespit edilmiştir. Ayrıca homonim çeşitlerin varlığı da ortaya çıkarılmıştır. Sonuçlar, SSR
markörlerinin asma çeşitlerinin parmak izi ve genetik çeşitlilik çalışmalarında etkin bir araç olduğunu göstermektedir.

Kaynakça

  • Alleweldt G & Possingham J V (1988). Progress in grapevine breeding. Theoretical and Applied Genetics 75: 669−673
  • Bowers J E, Dangl G S, Vignani R & Meredith C P (1996). Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39: 628-633
  • Bowers J E, Dangl G S & Meredith C P (1999). Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture 50(30): 243−246
  • Brown S M & Kresovich S (1996). Molecular characterization for plant genetic resource conservation. In: Paterson AH, ed. Genome mapping in plants. Austin, Texas: R.G. Landes Company, 85–9
  • Costantini L, Monaco A, Vouillamoz J F, Forlani M & Grando M S (2005). Genetic relationships among homonymous Vitis vinifera cultivars from Campania (Italy). Vitis 44: 25–34
  • De Mattia F, Lovicu G, Tardaguila J, Grassi F, Imazio S, Scienza A & Labra M (2009). Genetic relationships between Sardinian and Spanish viticulture: the case of ‘Cannonau’ and ‘Garnacha’. The Journal of Horticultural Science & Biotechnology 84: 65-71
  • Dilli Y (2008). Characterization of some important grape varieties, types and clones in the Aegean region by using icrosatellite (SSR) markers. PhD Thesis, Ege University, İzmir, 112 p (In Turkish)
  • Ergül A, Kazan K, Aras S, Çevik V, Çelik H & Söylemezoğlu G (2006). AFLP analysis of genetic variation within the two economically important Anatolian grapevine (Vitis vinifera L.) varietal groups. Genome 49: 467−475
  • GENRES (2014). Information System Genetic Resources. http://www.genres.de/vitis/ (2013) (Erişim tarihi: 1.05.2014)
  • Hizarci Y, Ercişli S, Yüksel C & Ergül A (2012). Genetic characterization and relatedness among autochthonous grapevine cultivars from Northeast Turkey by Simple Sequence Repeats (SSR). Journal of Applied Botany and Food Quality 85: 224−228
  • Karataş H, Değirmenci D, Velasco R, Vezzulli S, Bodur Ç & Ağaoğlu Y S (2007). Microsatellite fingerprinting of homonymous grapevine (Vitis vinifera L) varieties in neighboring regions of South-East Turkey. Scientia Horticulturae 114: 164–169
  • Leao P C S, Riaz S, Graziani R, Dangl G S & Motoike S Y (2009). Characterization of a Brazilian grape germplasm collection using microsatellite markers. American Journal of Enology and Viticulture 60: 517−524
  • Lodhi M A, Ye G N, Weeden N F & Reisch B I (1994). A simple and efficient method for DNA extraction from grapevine cultrivars and Vitis species. The Journal of Plant Molecular Biology Reporter 12: 6-13
  • OIV (2014). Descriptor list for grape varieties and Vitis species. 2nd Edition of the OIV. http://www.oıv.int: (Erişim tarihi: 01.05.2014)
  • Özcan S, Gürel E & Babaoğlu M (2001). Plant Biotechnology- Genetic Engineering and Its Applications. S.Ü. Foundation Publications pp. 456 (Turkish)
  • Regner F & Wiedeck E (2006). Identification of white Riesling clones by genetic markers. Vitis 39: 103−107
  • Rohlf F J (1998). NTSYS-pc numerical taxonomy and multivariate analysis system. Version 2.02. Exeter Publications Setauket, New York
  • Sefc K M, Regner F, Turetschek E, Gloessl J & Steinkellenr H (1999). Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome 42: 367-373
  • Scott K D, Eggler P, Seaton G, Rosetto M, Ablett E M, Lee L S & Henry R J (2000). Analysis of SSRs derived from grape ESTs. Theoretical and Applied Genetics 100: 723-726
  • Sneath P H A & Sokal R R (1973). Numerical Taxonomy. Freeman, San Francisco.
  • Şelli F, Bakır M, İnan G, Aygün H, Boz Y, Yaşasın A S, Özer C, Akman B, Söylemezoğlu G, Kazan K & Ergül A (2007). Simple sequence repeat-based assessment of genetic diversity in ‘Dimrit’ and ‘Gemre’ grapevine accessions from Turkey. Vitis 46(4): 182-187
  • Tangolar S G, Soydam S, Bakır M, Karaağaç E, Tangolar S & Ergül A (2009). Genetic analysis of grapevine cultivars from the eastern Mediterranean region of Turkey based on SSR markers. Tarım Bilimleri Dergisi – Journal of Agricultural Sciences 15: 1−8
  • This P, Jung A, Boccacci P, Borrego J, Botta R, Costantini L, Crespan M, Dangl G S, Eisenheld C, Fererira- Monterio F, Grando S, Ibanez J, Lacombe T, Laucou V, Magalhaes R, Meredith C P, Milani N, Peterlunger E, Regner F, Zulini L & Maul E (2004). Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics 109: 1448-1458
  • Thomas M R & Scott N S (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as sequence-tagged sites (STSs). Theoretical and Applied Genetics 86: 985−990
  • Vezzulli S, Michela T, Giuseppina C, Angelica J, Dustin C & Andrey Z (2008) A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses based on 283 SSR and 501 SNP-based markers. Theoretical and Applied Genetics 117(4): 499-511
  • VIVC (2007). Vitis International Variety Catalogue (http://www.vivc.de) (Erişim tarihi: 01.05.2014)
  • Yüksel C (2008). SSRs (Simple Sequence Repeats) based genetic characterization of Manisa, İzmir, Muğla and Kütahya grapevine germplasms. MSc. Thesis, Ankara University Biotechnology Institute, pp. 50 (In Turkish)

Characterization of Autochthonous Grapevine Cultivars (Vitis vinifera L.) from the Aegean Region of Turkey Using Simple Sequence Repeats (SSRs)

Yıl 2015, Cilt: 21 Sayı: 4, 538 - 545, 15.12.2015
https://doi.org/10.1501/Tarimbil_0000001355

Öz

Thirty-six autochthonous grapevine cultivars from the Aegean region of Turkey in addition to standard cultivars Cabernet
Sauvignon and Merlot (Vitis vinifera L.) were fingerprinted using SSR markers to assess their genetic relationships.
Eleven SSR primers produced successful amplifications and yielded 37 polymorphic bands. The number of bands per
primer changed between 2 and 6 while the number of polymorphic bands was between 2 and 3.6. Dice genetic similarity
coefficients ranged between 0.296 and 0.882 among the genotypes. The UPGMA dendrogram showed two main groups.
The first group was composed of Sultan Dimriti, Veyis and Güvercin Gözü cultivars. A large number of sub-groups were
placed in the second group which included the majority of autochthonous cultivars. The genetic differences among the
autochthonous cultivars and reference cultivars were clearly observed. The results showed that cultivars Siyah Razakı
and Parmak (0.96) were the most similar ones. Synonyms were identified for İnek Memesi and Ufak Dimrit cultivars.
In addition, homonymous cultivars were detected. SSR markers have proved to be an efficient tool for fingerprinting
grapevine cultivars and conducting genetic diversity studies.

Kaynakça

  • Alleweldt G & Possingham J V (1988). Progress in grapevine breeding. Theoretical and Applied Genetics 75: 669−673
  • Bowers J E, Dangl G S, Vignani R & Meredith C P (1996). Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39: 628-633
  • Bowers J E, Dangl G S & Meredith C P (1999). Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture 50(30): 243−246
  • Brown S M & Kresovich S (1996). Molecular characterization for plant genetic resource conservation. In: Paterson AH, ed. Genome mapping in plants. Austin, Texas: R.G. Landes Company, 85–9
  • Costantini L, Monaco A, Vouillamoz J F, Forlani M & Grando M S (2005). Genetic relationships among homonymous Vitis vinifera cultivars from Campania (Italy). Vitis 44: 25–34
  • De Mattia F, Lovicu G, Tardaguila J, Grassi F, Imazio S, Scienza A & Labra M (2009). Genetic relationships between Sardinian and Spanish viticulture: the case of ‘Cannonau’ and ‘Garnacha’. The Journal of Horticultural Science & Biotechnology 84: 65-71
  • Dilli Y (2008). Characterization of some important grape varieties, types and clones in the Aegean region by using icrosatellite (SSR) markers. PhD Thesis, Ege University, İzmir, 112 p (In Turkish)
  • Ergül A, Kazan K, Aras S, Çevik V, Çelik H & Söylemezoğlu G (2006). AFLP analysis of genetic variation within the two economically important Anatolian grapevine (Vitis vinifera L.) varietal groups. Genome 49: 467−475
  • GENRES (2014). Information System Genetic Resources. http://www.genres.de/vitis/ (2013) (Erişim tarihi: 1.05.2014)
  • Hizarci Y, Ercişli S, Yüksel C & Ergül A (2012). Genetic characterization and relatedness among autochthonous grapevine cultivars from Northeast Turkey by Simple Sequence Repeats (SSR). Journal of Applied Botany and Food Quality 85: 224−228
  • Karataş H, Değirmenci D, Velasco R, Vezzulli S, Bodur Ç & Ağaoğlu Y S (2007). Microsatellite fingerprinting of homonymous grapevine (Vitis vinifera L) varieties in neighboring regions of South-East Turkey. Scientia Horticulturae 114: 164–169
  • Leao P C S, Riaz S, Graziani R, Dangl G S & Motoike S Y (2009). Characterization of a Brazilian grape germplasm collection using microsatellite markers. American Journal of Enology and Viticulture 60: 517−524
  • Lodhi M A, Ye G N, Weeden N F & Reisch B I (1994). A simple and efficient method for DNA extraction from grapevine cultrivars and Vitis species. The Journal of Plant Molecular Biology Reporter 12: 6-13
  • OIV (2014). Descriptor list for grape varieties and Vitis species. 2nd Edition of the OIV. http://www.oıv.int: (Erişim tarihi: 01.05.2014)
  • Özcan S, Gürel E & Babaoğlu M (2001). Plant Biotechnology- Genetic Engineering and Its Applications. S.Ü. Foundation Publications pp. 456 (Turkish)
  • Regner F & Wiedeck E (2006). Identification of white Riesling clones by genetic markers. Vitis 39: 103−107
  • Rohlf F J (1998). NTSYS-pc numerical taxonomy and multivariate analysis system. Version 2.02. Exeter Publications Setauket, New York
  • Sefc K M, Regner F, Turetschek E, Gloessl J & Steinkellenr H (1999). Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome 42: 367-373
  • Scott K D, Eggler P, Seaton G, Rosetto M, Ablett E M, Lee L S & Henry R J (2000). Analysis of SSRs derived from grape ESTs. Theoretical and Applied Genetics 100: 723-726
  • Sneath P H A & Sokal R R (1973). Numerical Taxonomy. Freeman, San Francisco.
  • Şelli F, Bakır M, İnan G, Aygün H, Boz Y, Yaşasın A S, Özer C, Akman B, Söylemezoğlu G, Kazan K & Ergül A (2007). Simple sequence repeat-based assessment of genetic diversity in ‘Dimrit’ and ‘Gemre’ grapevine accessions from Turkey. Vitis 46(4): 182-187
  • Tangolar S G, Soydam S, Bakır M, Karaağaç E, Tangolar S & Ergül A (2009). Genetic analysis of grapevine cultivars from the eastern Mediterranean region of Turkey based on SSR markers. Tarım Bilimleri Dergisi – Journal of Agricultural Sciences 15: 1−8
  • This P, Jung A, Boccacci P, Borrego J, Botta R, Costantini L, Crespan M, Dangl G S, Eisenheld C, Fererira- Monterio F, Grando S, Ibanez J, Lacombe T, Laucou V, Magalhaes R, Meredith C P, Milani N, Peterlunger E, Regner F, Zulini L & Maul E (2004). Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics 109: 1448-1458
  • Thomas M R & Scott N S (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as sequence-tagged sites (STSs). Theoretical and Applied Genetics 86: 985−990
  • Vezzulli S, Michela T, Giuseppina C, Angelica J, Dustin C & Andrey Z (2008) A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses based on 283 SSR and 501 SNP-based markers. Theoretical and Applied Genetics 117(4): 499-511
  • VIVC (2007). Vitis International Variety Catalogue (http://www.vivc.de) (Erişim tarihi: 01.05.2014)
  • Yüksel C (2008). SSRs (Simple Sequence Repeats) based genetic characterization of Manisa, İzmir, Muğla and Kütahya grapevine germplasms. MSc. Thesis, Ankara University Biotechnology Institute, pp. 50 (In Turkish)
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Burçak İşçi

Yıldız Dilli Bu kişi benim

Yayımlanma Tarihi 15 Aralık 2015
Gönderilme Tarihi 21 Mayıs 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 21 Sayı: 4

Kaynak Göster

APA İşçi, B., & Dilli, Y. (2015). Characterization of Autochthonous Grapevine Cultivars (Vitis vinifera L.) from the Aegean Region of Turkey Using Simple Sequence Repeats (SSRs). Journal of Agricultural Sciences, 21(4), 538-545. https://doi.org/10.1501/Tarimbil_0000001355

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