Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

Year 2016, Volume: 22 Issue: 4, 548 - 554, 01.09.2016

Burcu Tuncer Arzu Çığ Ruhsar Yanmaz Fikret Yaşar

https://doi.org/10.1501/Tarimbil_0000001413

Cited By: 5

Abstract

Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment 32 °C and 35 °C for 2 days on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C , three genotypes of Turkish kale Balkaya, Yanmaz, Karadere 077 and five commercial F1 ornamental kale hybrids Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome . Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C

Keywords

Embryo, Genotype, High temperature, Microspore culture, White cabbage, Kale

Sıcaklık şoku uygulamaları Brassica türlerinde mikrospor embriyogenesisi uyarmak amacıyla yaygın olarak kullanılmaktadır

Abstract

. Bu çalışmada, 6 Türk beyaz baş lahana Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C , 3 Türk yaprak lahana Karadere 077, Balkaya, Yanmaz çeşit ve genotipi ile 5 ticari F1 hibrid süs lahanası çeşidinde Red Piegon, Victoria Piegon, Red Chidori, White Kamome, and Pink Kamome yüksek sıcaklık uygulamalarının 32 °C ve 35 °C, 2 gün mikrospor embriyogenesisi üzerine etkisi araştırılmıştır. Mikrospor kökenli embriyo oluşumu genotip ve yüksek sıcaklığa göre farklılık göstermiştir. En yüksek embriyo oluşumu 32 °C’de 9.92 embriyo petri-1 değeri ile Yalova-1 çeşidinden, 11.13 embriyo petri-1 değeri ile Pink Kamome F1 çeşidinden ve 35 °C’de ise 5.63 embriyo petri-1 Karadere 077 çeşidinden elde edilmiştir

Keywords

Embriyo, Genotip, Yüksek sıcaklık, Mikrospor kültürü, Beyaz baş lahana, Yaprak lahana

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