Abstract
Çoğaltılmış parçacık uzunluğu polimorfizmi AFLP markörleri, EcoRI, PstI ve MseI restriksiyon enzimleriyle birlikte 15 primer kombinasyonu kullanılarak, 34 adet yonca M. sativa klonu arasındaki genotipik varyasyonu araştırmak için kullanılmıştır. 34 adet klona özgü AFLP bandı gözlenmiştir. 15 primer çifti 460‟ı polimorfik olan toplam 1002 bant üretmiştir. Her primer kombinasyonu için tespit edilen polimorfik bantların sayısı 7 ile 67 arasında değişmiştir. Bunlara ilaveten 13 adet klonda da 22 adet klona özgü markör tespit edilmiştir. Veri analizi NTSYSpc version 2.1yazılımı ilegerçekleştirilmiştir. Genetik uzaklık değerleri 5.9374 ile 1.1453 arasında sıralanmıştır. En yüksek genetik çeşitlilik gösteren onbeş klon, yoncanın sentetik varyetelerini üretmek amacıyla seçilmiştir
References
- Bingham, E.T. 1980. Maximizing heterozygosity in autopolyploid. In: Polyploidy: Biological Relevance. (Eds. Lewis, W.H.) Plenum, New York, pp. 471–491.
- Doyle, J.J. and J.L. Doyle.1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemcal Bulletin 19: 11-15.
- Ferreira, D.F., A.C. Oliveira, M.X. Dos Santos, and M.A.P. Ramalho. 1995. Métodos de avaliação da divergência genética em milho e suas relações com os cruzamentos dialélicos. Pesquisa Agropecuária Brasileira 30: 1189-1194.
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- Kidwell, K.K., E.T. Bingham, D.R. Woodfield and T.C. Osborn. 1994a. Relationships among genetic distance, forage yield and heterozygosity in isogenic diploid and tetraploid alfalfa populations. Theoretical and Applied Genetics 89: 323-328.
- Kidwell, K.K., D.R. Woodfield, E.T. Bingham and T.C. Osborn. 1994b. Molecular marker diversity and yield of isogenic 2x and 4x single-crosses of alfalfa. Crop Science 34: 784-788.
- Labombarda, P., F. Pupilli and S. Arcioni. 2000. Optimal population size for RFLP-assisted cultivar identification in alfalfa (Medicago sativa L.). Agronomie 20: 233–240.
- Maureira, I.J., F. Ortega, H. Campos, and T.C. Osborn. 2004. Population structure and combining ability of diverse Medicago sativa germplasms. Theoretical and Applied Genetics 109: 775–782.
- Osborn, T.C., D.J. Brouwer and K.K. Kidwell. 1998. Exploiting genome differences for higher alfalfa forage yield. Proceedings of the North American Alfalfa Improvement Conference, Bozeman, MT. 95.
- Rohlf, J. 2000. Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Software, Applied Biostatistics Inc, New York, USA.
- Rumbaugh, M.D., J.L. Caddel and D.E. Rowe. 1988. Breeding and Quantitative genetics. In: Alfalfa and alfalfa improvement (Eds. Hanson, A.A., Barnes, D.K. and Hill, R.R.). Agronomy monograph no. 29. ASA, CSSA, and SSSA, Madison, Wis. 777–807.
- Segovia-Lerma, A., R.G. Cantrell, J.M. Conway and I.M. Ray. 2003. AFLP-based assessment of genetic diversity among nine alfalfa germplasms using bulk DNA templates. Genome 46: 51-58.
- Sumberg, J.E., R.P. Murphy and C.C. Lowe. 1983. Selection for fiber and protein concentration in a diverse alfalfa population. Crop Science 23: 11–14.
- Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de Lee, M. Hornes, A. Fritjers, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP - a new technique for DNAfingerprinting. Nucleic Acids Research 23: 4407–4.
Abstract
Amplified fragment length polymorphism AFLP markers were used to investigate genotypic variability among 34 alfalfa clones M. sativa using 15 primer combinations with restriction enzymes EcoRI, PstI and MseI. 34 unique AFLP fragments were observed. The 15 primer pairs produced a total of 1002 fragments of which 460 were polymorphic. The number of polymorphic fragments detected per primer combination ranged 7 to 67. Furthermore, 22 clone-specific markers were also detected in the 13 clones. Data analysis was performed with NTSYSpc version 2.1 software. Genetic distance values ranged 5.9374 to 1.1453. Fifteen clones which showed the highest genetic variation were selected for producing synthetic variety of Alfalfa
References
- Bingham, E.T. 1980. Maximizing heterozygosity in autopolyploid. In: Polyploidy: Biological Relevance. (Eds. Lewis, W.H.) Plenum, New York, pp. 471–491.
- Doyle, J.J. and J.L. Doyle.1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemcal Bulletin 19: 11-15.
- Ferreira, D.F., A.C. Oliveira, M.X. Dos Santos, and M.A.P. Ramalho. 1995. Métodos de avaliação da divergência genética em milho e suas relações com os cruzamentos dialélicos. Pesquisa Agropecuária Brasileira 30: 1189-1194.
- Moreno-Gonzales, J. and J.I. Cubero. 1994. Selection strategies and choise of breeding methods. In: Plant Breeding. (Eds. Hayward, M.D., Bosemark, N.O. and Omagosa, I.), Chapman and Hall, London, UK.
- Kidwell, K.K., E.T. Bingham, D.R. Woodfield and T.C. Osborn. 1994a. Relationships among genetic distance, forage yield and heterozygosity in isogenic diploid and tetraploid alfalfa populations. Theoretical and Applied Genetics 89: 323-328.
- Kidwell, K.K., D.R. Woodfield, E.T. Bingham and T.C. Osborn. 1994b. Molecular marker diversity and yield of isogenic 2x and 4x single-crosses of alfalfa. Crop Science 34: 784-788.
- Labombarda, P., F. Pupilli and S. Arcioni. 2000. Optimal population size for RFLP-assisted cultivar identification in alfalfa (Medicago sativa L.). Agronomie 20: 233–240.
- Maureira, I.J., F. Ortega, H. Campos, and T.C. Osborn. 2004. Population structure and combining ability of diverse Medicago sativa germplasms. Theoretical and Applied Genetics 109: 775–782.
- Osborn, T.C., D.J. Brouwer and K.K. Kidwell. 1998. Exploiting genome differences for higher alfalfa forage yield. Proceedings of the North American Alfalfa Improvement Conference, Bozeman, MT. 95.
- Rohlf, J. 2000. Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Software, Applied Biostatistics Inc, New York, USA.
- Rumbaugh, M.D., J.L. Caddel and D.E. Rowe. 1988. Breeding and Quantitative genetics. In: Alfalfa and alfalfa improvement (Eds. Hanson, A.A., Barnes, D.K. and Hill, R.R.). Agronomy monograph no. 29. ASA, CSSA, and SSSA, Madison, Wis. 777–807.
- Segovia-Lerma, A., R.G. Cantrell, J.M. Conway and I.M. Ray. 2003. AFLP-based assessment of genetic diversity among nine alfalfa germplasms using bulk DNA templates. Genome 46: 51-58.
- Sumberg, J.E., R.P. Murphy and C.C. Lowe. 1983. Selection for fiber and protein concentration in a diverse alfalfa population. Crop Science 23: 11–14.
- Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de Lee, M. Hornes, A. Fritjers, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP - a new technique for DNAfingerprinting. Nucleic Acids Research 23: 4407–4.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | May 1, 2009 |
| Submission Date | January 1, 2009 |
| Published in Issue | Year 2009 Volume: 15 Issue: 02 |
Journal of Agricultural Sciences is published open access journal. All articles are published under the terms of the Creative Commons Attribution License (CC BY).