Bazı Ayva Cydonia oblonga Mill. Genotiplerinde Yaprak Disklerinden Sürgün Organogenesisi

Year 2007, Volume: 13 Issue: 01, 54 - 62, 01.01.2007

Ahmet Aygün Hatice Dumanoğlu

https://doi.org/10.1501/Tarimbil_0000000455

Abstract

Bu çalışmanın amacı, bazı ayva Cydonia oblonga Mill. genotiplerinde yaprak disklerinden in vitro sürgün organogenesisini uyarmaktır. Çalışmada bitkisel materyal olarak, Quince A bodur , S.Ö. Sabahattin Özbek 39-200 bodur , S.Ö. 18-82 orta kuvvetli ve S.Ö. 58-315 kuvvetli klon anaçları ile Eşme, Limon ve Çukurgöbek çeşitleri kullanılmıştır. Organogenesis için iki denemede TDZ + NAA kombinasyonları ya da BA + NAA, AgNO3 ve putrasin uygulamalarının etkileri araştırılmıştır. Sürgün organogenesisi sadece 0.33 mg/l TDZ + 0.5 mg/l NAA + 2.0 mg/l AgNO3 içeren MS Murashige ve Skoog temel besin ortamında önemli düzeyde uyarılmıştır. Bu uygulama ile sürgün oluşturma oranı Quince A’da %80.0, S.Ö. 39-200’de %56.7, S.Ö. 18-82’de %17.5, S.Ö. 58-315’de %37.5, Eşme’de %36.7, Limon’da %40.0 ve Çukurgöbek’de %43.3 olarak belirlenmiştir. Ortalama sürgün sayısı ise genotiplere göre 0.4-2.7 adet/disk arasında değişmiştir

Keywords

Ayva, Cydonia oblonga Mill., in vitro, organogenesis, yaprak diski, sürgün

Shoot Organogenesis From Leaf Discs in Some Quince Cydonia oblonga Mill. Genotypes

Abstract

The objective of this study was to stimulate organogenesis of in vitro shoots from leaf discs in some quince Cydonia oblonga Mill. genotypes. Quince A dwarf , S.Ö. Sabahattin Özbek 39-200 dwarf , S.Ö. 18-82 semi dwarf and S.Ö. 58-315 vigorous clonal rootstocks, and Eşme, Limon, and Çukurgöbek cultivars were used as plant materials. Effects of TDZ + NAA combinations or BA + NAA, AgNO and putrescine treatments were investigated in two experiments for organogenesis. Shoot organogenesis was significantly induced on MS Murashige and Skoog basal medium containing 0.33 mg/l TDZ + 0.5mg/l NAA + 2.0 mg/l AgNO3. The rate of shoot formation was 80.0% in Quince A, 56.7% in S.Ö. 39-200, 17.5% in S.Ö. 18-82, 37.5% in S.Ö. 58-315, 36.7% in Eşme, 40.0% in Limon, and 43.3% in Çukurgöbek. The average shoot number per leaf disc was changed between 0.4 and 2.7 among genotypes

Keywords

Quince, Cydonia oblonga Mill., in vitro, organogenesis, leaf disc, shoot

References

• Bais, H. P., G. S. Sudha and G. A. Ravishankar. 2000. Putrescine and silver nitrate influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow Local. J. Plant Growth Regul. 19: 238-248.
• Baker, B. S. and S. K. Bhatia. 1993. Factors effecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga). Plant Cell, Tissue and Organ Culture 35: 273-277.
• Chevreau, E. and C. Leblay. 1993. The effect of mother plant pretreatment and explant choice on regeneration from in vitro pear leaves. Acta Horticulturae 336 : 263-268.
• Chevreau, E. and R. Bell. 2005. Pyrus spp. Pear and Cydonia spp. Quince. P. 543-565. In: R. E. Litz (ed.). Biotechnology of Fruit and Nut Crops. CABI Publishing, Trowbridge.
• Çelik, M. 1988. Ankara Koşullarında Williams, Ankara, Akça ve Şeker Armut Çeşitleri İçin En Uygun S.Ö. Ayva Anaçlarının Seçimi Üzerinde Bir Araştırma. Ankara Üniversitesi Ziraat Fakültesi Yayınları: 1075, Ankara.
• Dodds, J. H. and L. W. Roberts. 1993. Experiments in Plant Tissue Culture. Cambridge University Press, New York.
• Dolcet-Sanjuan, R., D. W. S. Mok and M. C. Mok. 1991. Plantlet regeneration from cultured leaves of Cydonia oblonga L. (quince). Plant Cell Reports 10: 240-242.
• Dumanoğlu H. ve A. Aygün. 1999. Seçilmiş bazı S.Ö. ayva anaçlarında mikroçeliklerin in vitro köklenmesi üzerine IBA, NAA ve karanlık uygulamalarının etkileri. Türkiye III. Ulusal Bahçe Bitkileri Kongresi. Bildiriler: 233-237. 14-17 Eylül 1999, Ankara.
• Evans, P. T. and R. L. Malmberg. 1989. Do polyamines have a role in plant development? Ann. Rev. Plant Physiol. Mol. Biol. 40: 235-269.
• Fasolo, F., R. H. Zimmerman and I. Fordham. 1989. Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell, Tissue and Organ Culture 16: 75-87.
• Gamage, N. and T. Nakanishi. 2000. İn vitro shoot regeneration from leaf tissue of apple (cultivar “Orine”): High shoot proliferation using carry over effect of TDZ. Acta Hort. 520: 291-298.
• Gülşen, Y. ve H. Dumanoğlu. 1992. Quince-A’nın in vitro çoğaltımında bazı oksinlerin ve BAP’nin sürgün verimi ve kalitesine etkileri. Türkiye I. Ulusal Bahçe Bitkileri Kongresi. Bildiriler (I): 291-294. 13-16 Ekim 1992, İzmir.
• Hatipoğlu, R. 1999. Bitki Biyotkenolojisi. Çukurova Üniv. Ziraat Fakültesi Yayınları: 190, Ders Kitabı: A-58, Adana.
• Heslop-Harrison, J. 2005. Introduction. P. XIX-XXIV. In: R. E. Litz (ed.). Biotechnology of Fruit and Nut Crops. CABI Publishing, Trowbridge.
• Hiratsuka, S. and M. Katagiri. 1988. Shoot and callus formation in explants from immature seeds of Japanese pear. Scientia Horticulturae 34 (3-4): 193-199.
• Jain, S. M. 2001. Tissue culture-derived variation in crop improvement. Euphytica 118: 153-166.
• James, D. J., A. J. Passey and E. Rugini. 1988. Factors affecting high frequency plant regeneration from apple leaf tissues cultured in vitro. J. Plant Physiol. 132: 148- 154.
• Korban, S. S. and R. M. Skirvin. 1985. İn vitro shoot regeneration from an intact and a sectioned embryo- axis of apple seeds. Plant Science 39 (1): 61-66.
• Litz, R. E. and D. J. Gray. 1992. Organogenesis and Somatic Embriyogenesis. P. 3-34. In: F. A. Hammerschlag and R. E. Litz (eds). Biotechnology of Perennial Fruit Crops. CABI, University Press, Cambridge.
• Mante, S., R. Scorza and J. M. Cordts. 1989. Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus. Plant Cell, Tissue and Organ Culture 19: 1-11.
• Murashige, T. and F. Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
• Pieterse, R. E. 1989. Regeneration of plants from callus and embryos of ‘Royal’ apricot. Plant Cell, Tissue and Organ Culture 19: 175-179.
• Predieri, S. and F. F. Malavasi. 1989. High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumila Mill.). Plant Cell, Tissue and Organ Culture 17: 133-142.
• Sarwar, M. and R. M. Skirvin. 1997. Effect of thidiazuron and 6-benzylaminopurine on adventitious shoot regeneration from leaves of three strains of ‘McIntosh’ apple (Malus x domestica Borkh.) in vitro. Scientia Hort. 68: 95-100.
• Schuerman, P. L. and A. M. Dandekar. 1993. Transformation of temperate woody crops: Progress and potentials. Scientia Hort. 55: 101-124.
• Sun, Q. R., Q. Z. Liu and R. H. Zhao. 2003. Somatic embryogenesis from in vitro leaves of pear. Acta Hort. Sinica 30 (1): 85-86.
• Swartz, H. J., R. Bors, F. Mohamed and K. Naess. 1990. The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves. Plant Cell, Tissue and Organ Culture 21: 179-184.
• Tornero, O. P., J. Egea, A. Vanoostende and L. Burgos. 2000. Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Science 158: 61-70.
• Welander, M. 1988. Plant regeneration from leaf and stem segments of shoot raised in vitro from mature apple trees. J. Plant Physiol. 132: 738-744.
• Welander, M. and G. Maheswaran. 1992. Shoot regeneration from leaf explants of dwarfing apple rootstocks. J. Plant Physiol. 140: 223-228.
• Yancheva, S.D. 1994. Preliminary studies on regeneration and transformation of plum (Prunus domestica L.). Acta Horticulturae 359: 159-163.