A Meta-analysis of Bentonite Efficacy on Performance, Carcass Yield, Giblet, and Blood Constituents of Broiler Fed Contaminated Aflatoxin

Year 2023, Volume: 29 Issue: 2, 395 - 403, 31.03.2023

Arif Darmawan , Anuraga Jayanegara Ergin Öztürk

https://doi.org/10.15832/ankutbd.970525

Abstract

Aflatoxins can easily grow and develop in many feed ingredients and influence the risk of several animal chronic diseases. The present study aimed to evaluate the effect of bentonite inclusion on performances, carcass yield, giblet, and blood constituents of broiler-fed contaminated aflatoxin through a meta-analytical approach. A database was developed based on scientific publications that were searched using several search tools such as Science Direct, PubMed, Scopus, and Google Scholar with “bentonite”, “clay”, “montmorillonite”, “aflatoxin” and “broiler” as keywords. A total of 31 studies were retrieved and included in the analysis. Data analysis was based on the mixed model in which dietary bentonite inclusion was treated as the fixed effect and different studies were considered as random effects. Bentonite used were sodium bentonite, calcium bentonite, and montmorillonite form with levels ranging from 0 to 5%. Meanwhile, aflatoxin B1 levels in the diet ranged from 0 to 5 mg/kg. In this study, body weight gain, feed intake, and feed efficiency showed a linear increase (p<0.001) in all phases. The mortality rate linearly decreased (p<0.05) in the cumulative. Bentonite inclusion linearly increased (p<0.001) the carcass, breast, leg, bursa of Fabricius, and gizzard weight proportion. The percentage of kidney, liver, heart, pancreas, uric acid, and gamma-glutamyltransferase linearly decreased (p<0.001) by increasing bentonite levels. Dietary bentonite linearly increased (p<0.001) total protein, albumin, aspartate aminotransferase, calcium, glycogen, globulin, and hemoglobin (p<0.05). It was concluded that bentonites have the ability to eliminate the detrimental effects of aflatoxin, which may increase the broiler performance, carcass yield and prevent the change in abnormal giblet weight. For effectiveness and animal safety, the EFSA recommends using a maximum of 2% bentonite in the feed.

Keywords

Aflatoxin, Bentonite, Body weight gain, Feed efficiency, Mixed model, Montmorillonite

Thanks

The authors would like to express our gratefulness to YTB Scholarship from the Turkey Government for providing a Ph.D. scholarship to the first author.

References

• Abadi M G M H, Moravej H, Shivazad M, Torshizi M A K & Kim W K (2019). Effects of feed form and particle size, and pellet binder on performance, digestive tract parameters, intestinal morphology, and cecal microflora populations in broilers. Poultry Science 98(3): 1432-1440. doi.org/10.3382/ps/pey488 
• Abeena B, George A J, Mohammed Shejir R, Nair N D & Manomohan C B (2015). Haematobiochemical changes of layer chicken in experimental aflatoxicosis. Indian Veterinary Journal 92(10): 84-86
• Aghashahi A R, Hosseinijangjo S H, Sadeghipanah H & Hosseini S A (2015). Effects of various types of bentonite (montmorillonite) on ascites-related physiologic and metabolic factors in broilers. Iranian Journal of Applied Animal Science 5(2): 423-430
• Alam S S & Deng Y (2017). Protein interference on aflatoxin B1 adsorption by smectites in corn fermentation solution. Applied Clay Science 144: 36-44. doi.org/10.1016/j.clay.2017.04.024
• Ani A O, Ogbu C C & Iloh E A (2014). Response of broiler chicks to diets containing graded levels of clay. Journal of Animal and Plant Science 24 (1): 30-34
• Attar A, Kermanshahi H & Golian A (2017). Effects of conditioning time and sodium bentonite on pellet quality, growth performance, intestinal morphology and nutrients retention in finisher broilers. British Poultry Science 59(2): 190-197. doi.org/10.1080/00071668.2017.1409422
• Attar A, Kermanshahi H, Golian A, Abbasipour A & Daneshmand A (2019). Conditioning time and sodium bentonite affect pellet quality, growth performance, nutrient retention and intestinal morphology of growing broiler chickens. British Poultry Science 60(6): 777-783. doi.org/10.1080/00071668.2019.1663493 
• Azizpour A & Moghadam N (2015). Effects of yeast glucomannan and sodium bentonite on the toxicity of aflatoxin in broilers. Brazilian Journal of Poultry Science 17: 7-13. doi.org/10.1590/1516-635xspecialissuenutrition-poultryfeedingadditives007-014 
• Bailey C A, Latimer G W, Barr A C, Wigle W L, Haq A U, Balthrop J E & Kubena L F (2006). Efficacy of montmorillonite clay (NovaSil PLUS) for protecting full-term broilers from aflatoxicosis. Journal of Applied Poultry Research 15(2): 198-206. doi.org/10.1093/japr/15.2.198
• Besseboua O, Ayad A, Hornıck J L & Benbarek H (2018). Dietary effects of Algerian sodium bentonite on growth performance and biochemıcal parameters in broiler chickens. Cercetări Agronomice în Moldova 4(176): 108-121
• Bryden W L (2012). Mycotoxin contamination of the feed supply chain: implications for animal productivity and feed security. Animal Feed Science and Technology 173(1-2): 134-158. doi.org/10.1016/j.anifeedsci.2011.12.014 
• Cruz B S, Patlan D H, Petrone V M, Pontin K P, Latorre J D, Beyssac E, Velasco X H, Guzman R M, Owens C, Hargis B M, Arellano R L & Isaias G T (2019). Evaluation of cellulosic polymers and curcumin to reduce aflatoxin B1 toxic effects on performance, biochemical, and ımmunological parameters of broiler chickens. Toxins 11(2): 121. doi.org/10.3390/toxins11020121 
• Damiri H, Chaji M, Bojarpour M & Mamuei, M (2012). Effect of different sodium bentonite levels on performance, carcass traits and passage rate of broilers. Pakistan Veterinary Journal 32(2): 197-200
• Damiri H, Chaji M, Bojarpour M, Eslami M & Mamoei M (2010). The effect of sodium betonites on economic value of broiler chickens diet. Journal of Animal Veterinary Advance 9(20): 2668-26670. doi.org/10.3923/javaa.2010.2668.2670
• Darmawan A & Ozturk E (2022). The impact of bentonite feed additives on laying hens performance and egg quality: a meta analysis. Iranian Journal of Applied Animal Science 12(4): 647-653
• Deng Y, Velázquez A L B, Billes F & Dixon J B (2010). Bonding mechanisms between Aflatoxin B1 and smectite. Applied Clay Science 50(1): 92-98. doi.org/10.1016/j.clay.2010.07.008 
• Dos Anjos F R, Ledoux D R, Rottinghaus G E & Chimonyo M (2015). Efficacy of Mozambican bentonite and diatomaceous earth in reducing the toxic effects of aflatoxins in chicks. World Mycotoxin Journal 9(1): 63-72. doi.org/10.3920/wmj2014.1842 
• Eckhardt J C, Santurio J M, Zanette R A, Rosa A P, Scher A, Dal Pozzo M, Alves S H & Ferreiro L (2014). Efficacy of a Brazilian calcium montmorillonite against toxic effects of dietary aflatoxins on broilers reared to market weight. Brtish Poultry Science 55(2): 215-220. doi.org/10.1080/00071668.2014.883065 
• EFSA (2012). Scientific opinion on the safety and efficacy of bentonite as a technological feed additive for all species. EFSA Journal 10(7): 2787
• Fouad A M, Ruan D, El-Senousey H K, Chen W, Jiang S & Zheng C (2019). Harmful effects and control strategies of Aflatoxin B1 Produced by Aspergillus flavus and Aspergillus parasiticus strains on poultry: review. Toxins (Basel) 11(3): 176. doi.org/10.3390/toxins11030176 
• Ghahri H, Habibian R & Fam M A (2010). Evaluation of the efficacy of esterified glucomannan, sodium bentonite, and humic acid to ameliorate the toxic effects of aflatoxin in broilers. Turkish Journal of Veterinary and Animal Science 34(4): 385-391. doi.org/10.3906/vet-0903-19 
• Gholami-Ahangaran M, Rangsaz N & Azizi S (2016). Evaluation of turmeric (Curcuma longa) effect on biochemical and pathological parameters of liver and kidney in chicken aflatoxicosis. Pharmaceutical Biology 54(5): 780-787. doi.org/10.3109/13880209.2015.1080731 
• Indresh H C, Devegowda G, Ruban S W & Shivakumar M C (2013). Effects of high grade bentonite on performance, organ weights and serum biochemistry during aflatoxicosis in broilers. Veterinary World 6(6): 313-317. doi.org/10.5455/vetworld.2013.313-317 
• Jaynes W F & Zartman R E (2011). Aflatoxin toxicity reduction in feed by enhanced binding to surface-modified clay additives. Toxins (Basel) 3(6): 551-565. doi.org/10.3390/toxins3060551 
• Katouli M S, Boldaji F, Daster B & Hassani S (2010). Effect of different level of koaline, bentonite and zeolite on broilers performance. Journal of Biological Sciences 10(1): 58-62. doi.org/10.3923/jbs.2010.58.62
• Kermanshahi H, Hazegh A R & Afzali N (2009). Effect of sodium bentonite in broiler chickens fed diets contaminated with Aflatoxin B1. Journal of Animal and Veterinary Advance 8(8): 1631-1636
• Khalifeh M J, Mohammadabadi T, Chaji M, Salari S & Khalil M (2012). The effect of different levels of sodium bentonite on in vitro fermentation and digestibility of soybean meal. In: Proceedings of the 15th AAAP Animal Science Congress; Thammasat University, Thailand. pp. 23-27
• Khanedar F, Vakili R & Zakizadeh S (2013). Effects of two kinds of bentonite on performance, blood biochemical parameters, carcass characteristics and tibia ash of broiler chickens. Iranian Journal of Applied Animal Science 3 (3): 577-581. https://doi.org/10.1186/2055-0391-56-20
• Ma Z, Long L H, Liu J & Cao Y X (2009). Montmorillonite adsorbs uric acid and increases the excretion of uric acid from the intestinal tract in mice. Journal of Pharmacy and Pharmacology 61(11): 1499-1504. doi.org/10.1211/jpp.61.11.0009 
• Malekinejad P, Afzali N, Mohammadi A & Sarir H (2015). Effects of combination of different levels sodium bentonite and Silybum marianum seeds on performance and carcass traits of broiler chicks fed diet contaminated with Aflatoxin B1 in starter and grower period. Journal of Applied Environmental and Biological Sciences 5(12): 269-275
• Manafi M (2012). Counteracting effect of high-grade sodium bentonite during aflatoxicosis in broilers. Journal of Agricultural Science and Technology 14(3): 539-547
• Mgbeahuruike A C, Nwoko I E, Nwaoga C T & Okorie-Kanu O (2018). Assessing the ameliorating effects of adsorbents on the health and performance of ducklings fed mold (Fungal) contaminated diets. International Journal of Plant, Animal and Environmental Sciences 10(2): 54-66
• Miazzo R, Peralta M F, Magnoli C, Salvano M, Ferrero S, Chiacchiera S M, Carvalho E C Q, Rosa C A R & Dalcero A (2005). Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumonisin. Poultry Science 84(1): 1-8. doi.org/10.1093/ps/84.1.1 
• Mohamadi H & Alizadeh M (2010). A Study of the occurrence of Aflatoxin M1 in dairy products marketed in Urimia, Iran. Journal of Agricultural Science and Technology 12(5): 579-583
• Nasaruddin N, Jinap S, Samsudin N I, Kamarulzaman N H & Sanny M (2021). Prevalence of mycotoxigenic fungi and assessment of aflatoxin contamination: a multiple case study along the integrated corn-based poultry feed supply chain in Malaysia. Journal of the Science of Food and Agriculture 101(5): 1812-1821 doi.org/10.1002/jsfa.10795 
• Pappas A C, Tsiplakou E, Georgiadou M, Anagnostopoulos C, Markoglou A N, Liapis K & Zervas G (2014). Bentonite binders in the presence of mycotoxins: Results of in vitro preliminary tests and an in vivo broiler trial. Applied Clay Science 99: 48-53. doi.org/10.1016/j.clay.2014.06.009 
• Park J H, Shin H J, Kim M H, Kim J S, Kang N, Lee J Y, Kim K T, Lee J I & Kim D D (2016). Application of montmorillonite in bentonite as a pharmaceutical excipient in drug delivery systems. Journal of Pharmaceutical Investigation 46(4): 363-375. doi.org/10.1007/s40005-016-0258-8 
• Pasha T N, Farooq M U, Khattak F M, Jabbar M A & Khan A D (2008). Effectiveness of sodium bentonite and two commercial products as aflatoxin absorbents in diets for broiler chickens. Animal Feed Science and Technology 132(1-2): 103-110. doi.org/10.1016/j.anifeedsci.2006.03.014
• Pasha T N, Mahmood A, Khattak F M, Jabbar M A & Khan A D (2007). The effect of feed supplemented with different sodium bentonite treatments on broiler performance. Turkish Journal of Veterinary and Animal Science 32(4): 245-248
• Pouraboulghasem H, Ghorbanpour M, Shayegh R & Lotfiman S (2016). Synthesis, characterization and antimicrobial activity of alkaline ion-exchanged ZnO/bentonite nanocomposites. Journal of Central South University 23: 787-792. doi.org/10.1007/s11771-016-3124-y 
• Rafiu T A, Babatunde G M, Ibrahim O O K, Akanbi A O & Ojelade R A (2019). Toxin and toxin-binders affecting the performance, organs, haematology and histological characteristics of broilers fed with infected diets. International Journal of Livestock Production 10(2): 33-42. doi.org/10.5897/ijlp2018.0536 
• Rathod P, Gangadhar K, Gangane G & Bhojane N (2017). Effect of aflatoxın on haematological and biochemical alteration in broilers. International Journal of Science Environment 6(1): 824-831
• Rosa C A, Miazzo R, Magnoli C, Salvano M, Chiacchiera S M, Ferrero S, Saenz M, Carvalho E C Q & Dalcero A (2001). Evaluation of the efficacy of bentonite from the south of Argentina to ameliorate the toxic effects of aflatoxin in broilers. Poultry Science 80(2): 139-144. doi.org/10.1093/ps/80.2.139 
• Safaeikatouli M, Boldaji F, Dastar B & Hassani S (2012). The effect of dietary silicate mineral supplementation on apparent ileal digestibility of energy and protein in broiler chickens. International Journal of Agriculture and Biology 14(2): 299-302
• Saleemi M K, Ashraf K, Gul S T, Naseem M N, Sajid M S, Mohsin M, He C, Zubair M & Khan A (2020). Toxicopathological effects of feeding aflatoxins B1 in broilers and its amelioration with indigenous mycotoxin binder. Ecotoxicology and Environmental Safety187: 109712. doi.org/10.1016/j.ecoenv.2019.109712 
• Shannon T A, Ledoux D R, Rottinghaus G E, Shaw D P, Daković A & Marković M (2017). The efficacy of raw and concentrated bentonite clay in reducing the toxic effects of aflatoxin in broiler chicks. Poultry Science 96(6): 1651-1658. doi.org/10.3382/ps/pew408 
• Shehata S A & El-Shafi S A (2011). Effect of copper bearing Egyptian bentonite on the growth performance and intestinal microflora of rabbits. The Journal of American Science 7(11): 72-78
• Shi Y H, Xu Z R, Feng J L & Wang C Z (2006). Efficacy of modified montmorillonite nanocomposite to reduce the toxicity of aflatoxin in broiler chicks. Animal Feed Science and Technology 129 (1-2): 138-148. doi.org/10.1016/j.anifeedsci.2005.12.006 
• Smith A D, Panickar K S, Urban Jr J F & Dawson H D (2018). Impact of micronutrients on the immune response of animals. Annual Review and Animal Biosciences 6: 227-254. doi.org/10.1146/annurev-animal-022516-022914 
• St-Pierre N (2001). Integrating quantitative findings from multiple studies using mixed model methodology. Journal of Dairy Science 84(4):741-755. doi.org/10.3168/jds.S0022-0302(01)74530-4
• Suzanne C A M, Marlia M H & Jalal A K C (2017). Environmental characteristics of clay and clay-based minerals. Geology Ecology and Landscape 1(3): 155-161. doi.org/10.1080/24749508.2017.1361128
• Tauqir N A, Sultan G I & Nawaz H (2001). Effect of different levels of bentonite with varying energy levels on the performance of broilers. International Journal of Agriculture and Biology 3(1): 85-88.
• Thalij K M (2005). Efficacy of activated sodium bentonite to reduce the toxicity of aflatoxins and T-2 toxin in broiler chicks. Mesopotamia Journal of Agriculture 33(4): 29-32. doi.org/10.33899/magrj.2005.34878 
• Umar S, Arshad A, Ahmad B & Arshad M (2012). Clinico biochemical and hematological changes in broilers induced by concurrent exposure to Aflatoxin B1 and Ochratoxin A. Journal of Public Health and Biological Sciences 1(3) :79-85
• Valchev I, Hristov T S, Lazarov L, Kanakov D, Binev R & Nikolov Y (2013). Investigations on production traits in broiler chickens with experimental aflatoxicosis. Bulgarian Journal of Veterinary Medicine 16 (4): 271-281
• Wang G, Lian C, Xi Y, Sun Z & Zheng S (2018). Evaluation of nonionic surfactant modified montmorillonite as mycotoxins adsorbent for aflatoxin B1 and zearalenone. Journal of Colloid and Interface Science 518: 48-56. doi.org/10.1016/j.jcis.2018.02.020 
• Wu Q J, Wang L C, Zhou Y M, Zhang J F & Wang T (2013). Effects of clinoptilolite and modified clinoptilolite on the growth performance, intestinal microflora, and gut parameters of broilers. Poultry Science 92(3): 684-692. doi.org/10.3382/ps.2012-02308 
• Xia M S, Hu C H & Xu Z R (2004). Effects of copper-bearing montmorillonite on growth performance, digestive enzyme activities, and intestinal microflora and morphology of male broilers. Poultry Science 83(11): 1868-1875. doi.org/10.1093/ps/83.11.1868