Evaluation of Human Papilloma Virus (HPV) Existance with Molecular Methods in Sexually Active Women

Abstract

The aim of this study was to determine HPV DNA by PCR in smear and biopsy materials in patients with abnormal smear and to compare the results with biopsy. The study included 24 patients aged 26 to 74 years. Patients with H-SIL, L-SIL or ASCUS who were diagnosed with abnormal smear during the pathological evaluation were evaluated and the presence of HPV was investigated with molecular tests. Demographic information was obtained from patients before colposcopy and the presence of factors that may cause HPV infection were examined. In pathological specimens taken from patients with abnormal smear results, biopsy was diagnosed as cervical CA in 33%, H-SIL in 4%, L-SIL in 13%, and normal in 50% of patients. HPV DNA was determined, and it was observed that HPV 16 was the highest prevalence of HPV subtypes (HPV 16, HPV 18, HPV 31 and HPV 58). Of the 5 patients with HPV 16, 4 were found to have cervical CA, while the remaining 1 was H-SIL. In addition, the HPV positivity rate was higher in people with more than 1 partner, whereas the number of HPV positive cases was three or more in gravity. In this study; the contribution of pathological diagnostic methods and microbiological results to the diagnosis, and the importance of the scanning programs and early diagnosis was revealed.

Keywords

• Cervical cancer,cervical intraepithelial neoplasia,HPV,smear

Human Papilloma Virüs (HPV) Varlığının Cinsel Aktif Kadınlarda Moleküler Metodlarla Değerlendirilmesi

Abstract

Bu çalışmada, anormal smear tanısı konan hastalarda, smear ve biyopsi materyallerinde PCR yöntemi ile HPV DNA analizi yapılarak sonuçların biyopsi tanısıyla karşılaştırılması amaçlanmıştır. Çalışma, yaşları 26 ile 74 arasında değişen 24 hasta üzerinde gerçekleştirilmiştir. Ele alınan vakalardan patolojik değerlendirme sonucunda H-SIL, L-SIL veya ASCUS ile anormal smear tanısı konmuş hastalara kolposkopik biyopsi yapılarak örnekler elde edilmiş ve moleküler testler ile HPV varlığı araştırılmıştır. Kolposkopi öncesinde hastalara anket uygulanarak demografik bilgiler elde edilmiş ve HPV enfeksiyonuna neden olabilecek faktörlerin varlığı incelenmiştir. Anormal smear sonucuna sahip hastalardan alınan patolojik örneklerde, biyopsi tanısı %33’ünde serviks CA, %4’ünde H-SIL, %13’ünde L-SIL ve %50’si normal olarak bulunmuştur. Ayrıca, normal smear sonucuna sahip tüm hastaların %33’ünde HPV DNA belirlenmiş ve belirlenen HPV alt tipleri (HPV 16, HPV 18, HPV 31 ve HPV 58) arasında en yüksek oranda izlenen alt tipin HPV 16 olduğu tespit edilmiştir. HPV 16 bulunan 5 hastadan 4’ünün biyopsi tanısının serviks CA olduğu tespit edilmiş, kalan 1 hastanın ise H-SIL olduğu görülmüştür. Ayrıca, partner sayısı 1’den fazla olan kişilerde HPV pozitiflik oranının yüksek olduğu görülürken, HPV pozitif vakaların tamamının gravide sayısının üç veya üzeri olduğu gözlenmiştir. Bu çalışmada patolojik tanı yöntemleri ve mikrobiyolojik inceleme sonuçlarının tanıya katkısı ortaya konulmuş, tarama programlarının ve erken teşhisin önemi bir kez daha ortaya çıkarılmıştır.

Keywords

• HPV,servikal intraepitelyal neoplazi,serviks kanseri,smear

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